Brazilian Journal of Microbiology, Год журнала: 2024, Номер 55(1), С. 255 - 267
Опубликована: Янв. 16, 2024
Язык: Английский
Frontiers in Microbiology, Год журнала: 2024, Номер 14
Опубликована: Янв. 8, 2024
The marine environment has remained a source of novel biological molecules with diversified applications. ecological and diversity, along unique physical environment, have provided the evolutionary advantage to plant, animals microbial species thriving in ecosystem. In light fact that microorganisms frequently interact symbiotically or mutualistically higher including corals, fish, sponges, algae, this paper intends examine potential as niche for bacteria. This review aims analyze summarize modern literature data on biotechnological fungi bacteria producers wide range practically valuable products (surfactants, glyco-and lipopeptides, exopolysaccharides, enzymes, metabolites different activities: antimicrobial, antitumor, cytotoxic). Hence, study bioactive secondary from is need hour. scientific novelty lies first time, new resources obtaining biologically active natural — were generalized. investigates various kinds derived microorganisms, specifically focusing products. It provides summary regarding antibacterial, antimalarial, anticarcinogenic, antibiofilm, anti-inflammatory effects demonstrated by microorganisms. There currently great applied research standpoint human animal health.
Язык: Английский
Процитировано
23
Marine Drugs, Год журнала: 2024, Номер 22(1), С. 51 - 51
Опубликована: Янв. 22, 2024
Natural alkaloids originating from actinomycetes and synthetic derivatives have always been among the important suppliers of small-molecule drugs. Among their biological sources, Streptomyces is highest most extensively researched genus. Marine-derived strains harbor unconventional metabolic pathways demonstrated to be efficient producers biologically active alkaloids; more than 60% these compounds exhibit valuable activity such as antibacterial, antitumor, anti-inflammatory activities. This review comprehensively summarizes novel produced by marine discovered in past decade, focusing on structural features, activity, pharmacological mechanisms. Future perspectives discovery development are also provided.
Язык: Английский
Процитировано
8
Frontiers in Microbiology, Год журнала: 2025, Номер 15
Опубликована: Янв. 6, 2025
Marine-derived actinobacteria isolated from sponge Cliona varians and soft coral Eunicea fusca were screened for antibacterial activity against acne-related bacteria, specifically Staphylococcus epidermidis ATCC 14990, methicillin-resistant aureus BAA44, Cutibacterium acnes 6919. Cytotoxicity assays performed on human dermal fibroblast (HDFa) keratinocyte (HaCaT) cell lines to assess the safety profile of extracts. Chemical characterization was conducted using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Among extracts, six derived Kocuria sp., Rhodococcus Nocardia Micrococcus Streptomyces sp. demonstrated significant activity. Notably, extract Z9.216 exhibited highest efficacy, inhibiting S. by 68%, 93%, C. 98.7% at a concentration 0.003 mg/mL, which comparable standard antibiotics erythromycin vancomycin, while maintaining over 90% viability in both HDFa HaCaT lines. Untargeted metabolomic analysis suggested that might be associated compounds chemical families alkaloids, terpenoids, fatty acids, among others. These findings highlight therapeutic potential marine underexplored environments as promising strategy treating acne vulgaris, chronic inflammatory skin condition.
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Янв. 9, 2025
Abstract Biosynthesized nanoparticles have a variety of applications, and microorganisms are considered one the most ideal sources for synthesis green nanoparticles. Icerya aegyptiaca (Douglas) is pest that has many generations per year can affect 123 plant species from 49 families by absorbing sap bark, forming honeydew, causing sooty mold, attracting invasive ant species, leading to significant agricultural losses. The purpose this work was synthesize titanium dioxide (TiO 2 -NPs) marine actinobacteria evaluate their insecticidal effects on (Hemiptera: Monophlebidae), in addition explaining protein electrophoresis analysis SDS‒PAGE proteins control treated insects after 24, 72 120 h exposure. In all, seven actinobacterial isolates, potent which potential produce hydroxide-based -NP2), DNA sequences 99.9% like those Streptomyces rubrolavendulae (MCN2) according nucleotide alignment phylogenetic tree. produced TiO -NPs were verified UV examination characterized FT-IR, XRD, TEM, EDX, DLS analyses. Toxicological results revealed high mortality rates reaching 55, 62.5, 80 95% at TiO2-NPs dose 120,250,500 1000 ppm respectively. Compared with control, TiO2-NP spraying caused changes pattern I. aegyptiaca, as indicated disappearance normal bands appearance other bands, well quantitative qualitative content application MNC2 offers new alternative strategy modern approach nanotechnology.
Язык: Английский
Процитировано
0
Brazilian Journal of Microbiology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 29, 2025
Язык: Английский
Процитировано
0
Frontiers in Marine Science, Год журнала: 2025, Номер 12
Опубликована: Фев. 13, 2025
Oceans boast a substantial microbial diversity, which is widely prevalent in seawater, marine sediments, and organisms. In contrast to terrestrial resources explored traditional natural product research, the habitats of microorganisms are distinctly unique. Actinomycetes serve as vital source secondary metabolites, including antibiotics other potent products like streptomycin tetracycline. They have played pivotal role clinical treatments for significant diseases such pathogenic bacterial infections. Nevertheless, extensive use has led sharp increase variety number drug-resistant bacteria, notably multidrug-resistant (MDR) extensively (XDR) settings, posing grave threat human survival. Consequently, there an immediate need discover structurally novel antibacterial develop new antibiotics. This mini review summarizes total 45 derived from actinomycetes, published 2024. These products, polyketides, alkaloids, macrolactams, peptides, highlighted terms their structures biological activities. The objective this article provide valuable insights research development
Язык: Английский
Процитировано
0
Aquaculture Reports, Год журнала: 2025, Номер 41, С. 102690 - 102690
Опубликована: Фев. 15, 2025
Язык: Английский
Процитировано
0
Synthetic and Systems Biotechnology, Год журнала: 2025, Номер 10(2), С. 583 - 592
Опубликована: Фев. 20, 2025
Microorganisms are often likened to complex production workshops. In Saccharopolyspora spinosa (S. spinosa), the biosynthesis of spinosad is a line within its intricate workshop. Optimizing entire environment and reducing unnecessary metabolic flow essential increasing yield. Pyruvate serves as crucial precursor for biosynthesis. Previous studies revealed that pyc gene highly expressed in gluconeogenic pathway, leading pyruvate shunt. By downregulating pyc, we enhanced yield, although improvement was below expectations. We speculated most accumulated following knockdown entered some synthetic pathways unrelated spinosad. Through pathway qRT-PCR analyses, found expression levels gltA1 atoB3 tributary, including TCA cycle ethylmalonyl-CoA were significantly increased strain. The combined these three genes optimized line, yield 633.1 ± 38.6 mg/L, representing 199.4 % increase. This study identifies key optimizing offers insights into screening efficient construction Spinosad-producing strains.
Язык: Английский
Процитировано
0
Microbiology, Год журнала: 2025, Номер 94(1), С. 134 - 138
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Marine Drugs, Год журнала: 2025, Номер 23(3), С. 115 - 115
Опубликована: Март 7, 2025
Marine microplastics (MPs) represent a novel ecological niche, populated by fungi with high potential for pharmaceutical discovery. This study explores the bioactivity of fungal strains isolated from MPs in Mediterranean sediments, focusing on their osteogenic and antiviral activities. Crude extracts prepared via solid-state submerged-state fermentation were tested effects extracellular matrix mineralization vitro bone growth zebrafish larvae, activity against respiratory syncytial virus (RSV) herpes simplex type 2 (HSV-2). Several exhibited significant mineralogenic activities, Aspergillus jensenii MUT6581 Cladosporium halotolerans MUT6558 being most performing ones. Antiviral assays identified A. Bjerkandera adusta MUT6589 as effective RSV HSV-2 at different extents, no cytotoxic effect. Although chemical profiling extract led to isolation decumbenones A B, they did not reproduce observed bioactivities, suggesting involvement other active compounds or synergistic effects. These results highlight plastisphere valuable resource bioactive suggest need further fractionation characterization identify molecules responsible these promising
Язык: Английский
Процитировано
0