Olive Mill Wastewater Extract: In Vitro Genotoxicity/Antigenotoxicity Assessment on HepaRG Cells
International Journal of Environmental Research and Public Health,
Год журнала:
2024,
Номер
21(8), С. 1050 - 1050
Опубликована: Авг. 9, 2024
Olive
mill
wastewater
(OMWW),
with
its
high
level
of
phenolic
compounds,
simultaneously
represents
a
serious
environmental
challenge
and
great
resource
potential
nutraceutical
activities.
To
increase
the
knowledge
OMWW's
biological
effects,
an
aim
to
developing
food
supplement,
we
performed
chemical
characterisation
extract
using
Liquid
Chromatography-Quadrupole
Time-of-flight
spectrometry
(LC-QTOF)
in
vitro
genotoxicity/antigenotoxicity
assessment
on
HepaRG
™
cells.
Chemical
analysis
revealed
that
most
abundant
compound
was
hydroxytyrosol.
Biological
tests
showed
not
cytotoxic
at
lowest
tested
concentrations
(from
0.25
2.5
mg/mL),
unlike
highest
5
20
mg/mL).
Regarding
genotoxic
activity,
when
non-cytotoxic
concentrations,
did
display
any
effect.
Additionally,
OMWW
antigenotoxic
activity
(J-shaped
dose-response
effect)
against
known
mutagenic
substance,
reducing
extent
DNA
damage
co-exposure
treatment.
The
effect
also
obtained
post-exposure
procedure,
although
only
0.015625
0.03125
mg/mL.
This
behaviour
confirmed
pre-exposure
protocol.
In
conclusion,
present
study
established
maximum
non-toxic
dose
for
cell
model,
smoothing
path
future
research.
Язык: Английский
Control of Listeria monocytogenes on Frankfurters by Surface Treatment with Olive Mill Wastewater Polyphenolic Extract
Foods,
Год журнала:
2025,
Номер
14(5), С. 774 - 774
Опубликована: Фев. 24, 2025
Listeria
monocytogenes
(LM)
is
a
frequent
post-process
contaminant
in
meat
products.
This
study
aimed
to
investigate
the
antilisterial
effectiveness
of
antimicrobial
treatments
employing
olive
mill
wastewater
polyphenolic
extract
(PE)
commercially
manufactured
frankfurters.
Frankfurters
were
inoculated
on
surface
with
three-strain
LM
mixture
(~102
CFU/g),
treated
PE
2-fold
series
concentrations
(PM,
2PM,
4PM)
and
control
group
(CTR)
PE-untreated
samples.
Then,
frankfurters
vacuum-packed
stored
at
4
°C
for
28
days.
Samples
examined
days
0,
7,
14,
21,
count,
silico
growth
modeling,
impacts
pH,
water
activity
(aw),
sensory
characteristics.
From
T(time)
treatment
showed
significant
effect
growth,
registering
maximum
Δ
values
between
CTR
4PM
3.19,
4.86,
4.59,
4.39
T7,
T14,
T21,
T28,
respectively.
Minimum
was
attributable
PM
(CTR
versus
PM)
2.07,
2.52,
1.14,
0.65
No
changes
occurred
pH
(average
6
T0
5.9
T28),
aw
0.978
0.968
nor
profile
(p
>
0.05).
These
findings
suggest
that
an
effective
natural
antimicrobial,
offering
promising
approach
enhancing
food
safety
extending
shelf
life
Язык: Английский
Enhanced Lipid Yield from Olive-Mill Wastewater by Yarrowia lipolytica NRRL YB-423
Journal of Agricultural Production,
Год журнала:
2025,
Номер
6(1), С. 32 - 40
Опубликована: Март 26, 2025
Lipid
production
from
olive-mill
wastewater
(OMW)
by
Yarrowia
lipolytica
NRRL
YB-423
was
optimized
(biomass
concentration
and
lipid
yield
based
on
dry
cell
weight)
using
multi-response
criteria
the
Taguchi
orthogonal
array.
Sixteen
experimental
runs
were
performed
L16
Dilution
rates
of
OMW
(15,
30,
45,
60%),
Tween
80
(0,
0.2,
0.4,
0.6%),
sodium
chloride
(NaCl;
0,
1,
2,
3%),
sterility
selected
as
factors.
The
significance
parameters
determined
analysis
variance
(ANOVA).
effects
all
factors
statistically
significant
(p<0.05).
results
showed
that
had
a
maximum
contribution
48.12%
to
yield.
highest
(40.88
%)
achieved
in
sterile
medium
supplemented
with
15%
diluted
OMW,
0.6%
80,
3%
NaCl.
Язык: Английский
Exploring plant-based resources: A frontier in combating against common bacterial and fungal pathogens
Elsevier eBooks,
Год журнала:
2025,
Номер
unknown, С. 303 - 326
Опубликована: Янв. 1, 2025
Язык: Английский
Evaluation of the Antimicrobial Effects of Olive Mill Wastewater Extract Against Food Spoiling/Poisoning, Fish-Pathogenic and Non-Pathogenic Microorganisms
Microorganisms,
Год журнала:
2024,
Номер
12(11), С. 2216 - 2216
Опубликована: Окт. 31, 2024
Although
antibiotics
are
the
main
therapy
for
bacterial
infections,
reports
showed
that
overuse
(or
misuse)
of
will
results
in
several
problems
such
as
development
antibiotic-resistant
strains,
persistence
drug
residues,
and
numerous
environmental
concerns.
Therefore,
finding
antibiotic
alternatives
is
considered
vital
importance.
Investigation
antimicrobial
properties
plant
substances
extracts
great
value
to
replace
antibiotics.
With
this
objective,
study
aimed
evaluate
activities
an
ethanolic
extract
prepared
from
olive
mill
wastewater
(OMWW),
which
a
by-product
oil
production
with
considerable
burden,
against
38
including
fish-associated
pathogens,
non-pathogenic
isolates,
collection
one
yeast
strain,
Язык: Английский