Molecules, Год журнала: 2024, Номер 29(19), С. 4590 - 4590

Опубликована: Сен. 27, 2024

Nano- and micro-sized vesicular colloidal structures mediate cell–cell communication. They are important players in the physiology of plants, animals, humans, a subject increasing interest. We investigated effect three surfactants, N-cetylpyridinium chloride (CPC), sodium dodecyl sulfate (SDS), Triton X-100 (TX100), two anionic polyelectrolytes, polystyrene sulfonate (NaPSS) polymethacrylate (NaPMA), on nanoliposomes. In addition, SDS TX100 selected biological membranes (erythrocytes microalgae) was investigated. The liposomes were produced by extrusion evaluated microcalorimetry light scattering, based total intensity scattered (Itot), hydrodynamic radius (Rh), gyration (Rg), shape parameter p (=Rh/Rg,0), polydispersity index. EPs shed from erythrocytes microalgae Dunaliella tertiolecta Phaeodactylum tricornutum visualized scanning electron microscopy (SEM) analyzed flow cytometry (FCM). Rh Itot values POPC liposome suspensions with added CPC, SDS, roughly constant up to respective critical micelle concentrations (CMCs) surfactants. At higher compound concentrations, dropped towards zero, whereas increased than pure (Rh ≈ 60–70 nm), indicating disintegration formation larger particles, i.e., various POPC–S aggregates. Nanoliposomes stable upon addition NaPSS NaPMA, as indicated values. interaction or exothermic, while there no measurable heat effects NaPMA. number density several-fold erythrocyte 30-fold conditioned media at expense cells, which decreased less 5% tertiolecta. did not affect tricornutum, lower control, but concentration-dependent manner. Our results indicate that amphiphilic molecules need be organized nanosized particles match local curvature membrane for facilitated uptake. To pursue this hypothesis, other surfactants should studied future more general conclusions.

Язык: Английский