Current and Near-Future Technologies to Quantify Nanoparticle Therapeutic Loading Efficiency and Surface Coating Efficiency with Targeted Moieties

Bioengineering, Год журнала: 2025, Номер 12(4), С. 362 - 362

Опубликована: Март 31, 2025

Active targeting nanoparticles are a new generation of drug and gene delivery systems with the potential for greatly improved therapeutics compared to conventional nanomedicine approaches. Despite their potential, translation active faces challenges in production scale-up batch consistency. Accurate quality control methods nanoparticle therapeutic payload coating characterization critical attaining desired levels repeatability, drug/gene loading efficiency, molecule effectiveness, safety profiles. Current limitations technologies, such as relying on ensemble-average analysis, pose assessing content surface modification heterogeneity, which can affect outcomes. Single-molecule analysis technologies have emerged promising alternative, offering rich information heterogeneity stochastic variations between batches. This review first evaluates identifies traditional tools that rely indirect, bulk solution quantification methods. Subsequently, newly emerging introduced targeted moiety efficiencies single-nanoparticle resolution, help guide researchers towards advancing into clinical setting.

Язык: Английский

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Targeted therapy using nanocomposite delivery systems in cancer treatment: highlighting miR34a regulation for clinical applications

Cancer Cell International, Год журнала: 2023, Номер 23(1)

Опубликована: Май 6, 2023

Abstract The clinical application of microRNAs in modern therapeutics holds great promise to uncover molecular limitations and conquer the unbeatable castle cancer metastasis. miRNAs play a decisive role that regulating gene expression at post-transcription level while controlling both stability translation capacity mRNAs. Specifically, miR34a is master regulator tumor suppressor gene, progression, stemness, drug resistance cell p53-dependent independent signaling. With changing, trends nanotechnology, particular with revolution field nanomedicine, nano delivery systems have emerged as prominent strategy practices coupled delivery. Recently, it has been observed forced human lines model organisms limits proliferation metastasis by targeting several signaling cascades, various studies endorsing deregulation cells modulates apoptosis thus requires targeted nano-delivery for treatment. In this sense, present review aims provide an overview applications regulation therapy cancer.

Язык: Английский

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Leveraging efferocytosis blockade for improved cancer chemo-immunotherapy through synchronized release of doxorubicin and BMS777607 confined within tailored mesoporous silica nanoparticles

Nano Today, Год журнала: 2023, Номер 53, С. 102039 - 102039

Опубликована: Окт. 21, 2023

Язык: Английский

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Harnessing the synergistic potential of NK1R antagonists and selective COX-2 inhibitors for simultaneous targeting of TNBC cells and cancer stem cells

Journal of drug targeting, Год журнала: 2024, Номер 32(3), С. 258 - 269

Опубликована: Янв. 22, 2024

Triple-negative breast cancer (TNBC) lacks the expression of oestrogen receptor (ER), progesterone (PR), and human epidermal growth factor 2 (HER2), rendering it unresponsive to endocrine therapy HER2 targeted treatments. Though certain chemotherapeutics targeting cell cycle have shown efficacy a extent, presence chemotherapy-resistant stem cells (CSCs) presents significant challenge in tackling TNBC. Multiple lines evidence suggest upregulation neuropeptide Substance P (SP), its NK-1 (NK1R) Cyclooxygenase-2 (COX-2) enzyme TNBC patients. Upregulation SP/NK1R system COX-2 influences major signalling pathways involved proliferation, growth, survival, angiogenesis, inflammation, metastasis activity. The simultaneous activation crosstalk between activated by consequently increase levels key regulators self-renewal CSCs, promoting stemness. combination with NK1R antagonists inhibitors can simultaneously target thereby enhancing treatment reducing risk recurrence relapse. This review discusses rationale for combining better management novel strategy deliver drug cargo precisely tumour site address challenges associated off-target binding.

Язык: Английский

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Peptide nanocarriers co-delivering an antisense oligonucleotide and photosensitizer elicit synergistic cytotoxicity

Journal of Colloid and Interface Science, Год журнала: 2024, Номер 664, С. 338 - 348

Опубликована: Март 5, 2024

Combination therapies demand co-delivery platforms with efficient entrapment of distinct payloads and specific delivery to cells possibly organelles. Herein, we introduce the combination two therapeutic modalities, gene photodynamic therapy, in a purely peptidic platform. The simultaneous formation cargo loading multi-micellar platform is governed by self-assembly at nanoscale. architecture nanocarrier positive charge its constituent micelles offer controlled dual capacity locations for hydrophobic photosensitizer (PS) negatively charged antisense oligonucleotides (ASOs). Moreover, nuclear localization signal (NLS) sequence built-in peptide targets PS + ASO-loaded nanocarriers nucleus. Breast cancer treated demonstrated photo-triggered enhancement radical oxygen species (ROS) associated increased cell death. Besides, ASO resulted up 90 % knockdown Bcl-2, an inhibitor apoptosis that overexpressed more than half all human cancers. Simultaneous elicited synergistic extent could not be reached singly loaded or free form drugs. Both, location compounds prevents them from interfering each other, highly cellular support great potential this versatile therapy.

Язык: Английский

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