RAS70 peptide targets multiforme glioblastoma by binding to the plasma membrane heat shock protein HSP70 DOI Creative Commons
Maxim Shevtsov, Natalia Yudintceva, Danila Bobkov

и другие.

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Март 24, 2025

Multiforme glioblastoma-homing peptides, particularly targeting plasma membrane-bound heat shock protein mHsp70, demonstrate great application potential for tumor theranostics. In the current study, to further increase bioavailability as well penetration capacity through blood-brain barrier (BBB) of mHsp70-targeted peptide TKDNNLLGRFELSG, which is known bind oligomerization sequence mHsp70 chaperone, latter was conjugated with tripeptide RGD (forming chimeric termed RAS70). model BBB system RAS70 efficiently crossed accumulating in glioblastoma cells. Subsequently, orthotopic glioma models, intravenous administration fluorescently labeled agent (RAS70-sCy7.5) resulted retention (further confirmed by histological studies). Thus, shown biodistribution studies employing epifluorescence imaging, accumulation RAS70-sCy7.5 C6 significantly enhanced compared scramble peptide. Local that sprayed over dissected brain tissues helped delineate tumors glioma-bearing animals an intraoperative fluorescent imaging system. Tumor-specific internalization on ex vivo primary GBM samples obtained from adult neurooncological patients. conclusion, demonstrated high glioma-homing properties could be employed visualization developing a carrier drug delivery.

Язык: Английский

RAS70 peptide targets multiforme glioblastoma by binding to the plasma membrane heat shock protein HSP70 DOI Creative Commons
Maxim Shevtsov, Natalia Yudintceva, Danila Bobkov

и другие.

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Март 24, 2025

Multiforme glioblastoma-homing peptides, particularly targeting plasma membrane-bound heat shock protein mHsp70, demonstrate great application potential for tumor theranostics. In the current study, to further increase bioavailability as well penetration capacity through blood-brain barrier (BBB) of mHsp70-targeted peptide TKDNNLLGRFELSG, which is known bind oligomerization sequence mHsp70 chaperone, latter was conjugated with tripeptide RGD (forming chimeric termed RAS70). model BBB system RAS70 efficiently crossed accumulating in glioblastoma cells. Subsequently, orthotopic glioma models, intravenous administration fluorescently labeled agent (RAS70-sCy7.5) resulted retention (further confirmed by histological studies). Thus, shown biodistribution studies employing epifluorescence imaging, accumulation RAS70-sCy7.5 C6 significantly enhanced compared scramble peptide. Local that sprayed over dissected brain tissues helped delineate tumors glioma-bearing animals an intraoperative fluorescent imaging system. Tumor-specific internalization on ex vivo primary GBM samples obtained from adult neurooncological patients. conclusion, demonstrated high glioma-homing properties could be employed visualization developing a carrier drug delivery.

Язык: Английский

Процитировано

0