Deciphering Aspergillus section Terrei in Galleria mellonella model: a clade-specific pathogenicity characterization DOI Creative Commons
Roya Vahedi-Shahandashti, Jos Houbraken, Vít Hubka

и другие.

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

ABSTRACT The Aspergillus genus encompasses a diverse array of species, some which are opportunistic pathogens. Traditionally, human aspergillosis has primarily been linked to few predominantly A. fumigatus . Changes in epidemiology and advancements molecular techniques have brought attention less common previously unrecognized pathogenic cryptic species. Despite the taxonomic recognition many species section Terrei , their virulence potential clinical implications, compared terreus sensu stricto remain poorly understood. Hence, current study utilized alternative vivo model Galleria mellonella evaluate 19 accepted classified into three series (major phylogenetic clades): Nivei Ambigui Analyzing median survival rates infected larvae all each revealed that significantly lower Taking closer look at trend within clade, dividing two groups: highly virulent (up 72 h survival) 144 survival). Histological observation, considering fungal distribution filamentation, further supported this assessment, revealing increased hyphal formation Additionally, susceptibility profile conventional antifungals was determined, an azole minimum inhibitory concentration for tested such as niveus iranicus Our results highlight importance identification, they can exhibit different levels show reduced antifungal susceptibility. IMPORTANCE With changing increasingly vulnerable population, emerging Their diversity relevance underexplored, with showing higher virulence, highlighting need better preparedness practice. Using model, we assessed including non-cryptic across significant variation among series, displaying high virulence. analysis confirmed spread more elevated concentrations were also observed certain This presents novel insights pathogenicity emphasizing critical accurately identifying due resistance, may substantial implications.

Язык: Английский

Deciphering Aspergillus section Terrei in Galleria mellonella model: a clade-specific pathogenicity characterization DOI Creative Commons
Roya Vahedi-Shahandashti, Jos Houbraken, Vít Hubka

и другие.

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

ABSTRACT The Aspergillus genus encompasses a diverse array of species, some which are opportunistic pathogens. Traditionally, human aspergillosis has primarily been linked to few predominantly A. fumigatus . Changes in epidemiology and advancements molecular techniques have brought attention less common previously unrecognized pathogenic cryptic species. Despite the taxonomic recognition many species section Terrei , their virulence potential clinical implications, compared terreus sensu stricto remain poorly understood. Hence, current study utilized alternative vivo model Galleria mellonella evaluate 19 accepted classified into three series (major phylogenetic clades): Nivei Ambigui Analyzing median survival rates infected larvae all each revealed that significantly lower Taking closer look at trend within clade, dividing two groups: highly virulent (up 72 h survival) 144 survival). Histological observation, considering fungal distribution filamentation, further supported this assessment, revealing increased hyphal formation Additionally, susceptibility profile conventional antifungals was determined, an azole minimum inhibitory concentration for tested such as niveus iranicus Our results highlight importance identification, they can exhibit different levels show reduced antifungal susceptibility. IMPORTANCE With changing increasingly vulnerable population, emerging Their diversity relevance underexplored, with showing higher virulence, highlighting need better preparedness practice. Using model, we assessed including non-cryptic across significant variation among series, displaying high virulence. analysis confirmed spread more elevated concentrations were also observed certain This presents novel insights pathogenicity emphasizing critical accurately identifying due resistance, may substantial implications.

Язык: Английский

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