Genistein, a tool for geroscience

Mechanisms of Ageing and Development, Год журнала: 2022, Номер 204, С. 111665 - 111665

Опубликована: Март 17, 2022

Язык: Английский

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New Mediators in the Crosstalk between Different Adipose Tissues

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(9), С. 4659 - 4659

Опубликована: Апрель 25, 2024

Adipose tissue is a multifunctional organ that regulates many physiological processes such as energy homeostasis, nutrition, the regulation of insulin sensitivity, body temperature, and immune response. In this review, we highlight relevance different mediators control adipose activity through systematic review main players present in white brown tissues. Among them, inflammatory secreted by tissue, classical adipokines more recent ones, elements system infiltrated into (certain cell types interleukins), well role intestinal microbiota derived metabolites, have been reviewed. Furthermore, anti-obesity promote activation beige e.g., myokines, thyroid hormones, amino acids, both long micro RNAs, are exhaustively examined. Finally, also analyze therapeutic strategies based on those described to date. conclusion, novel regulators obesity, microRNAs or microbiota, being characterized promising tools treat obesity future.

Язык: Английский

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Association Between Plasma Redox State/Mitochondria Function and a Flu-Like Syndrome/COVID-19 in the Elderly Admitted to a Long-Term Care Unit

Frontiers in Physiology, Год журнала: 2021, Номер 12

Опубликована: Дек. 15, 2021

Background/Aims: It is widely known that the imbalance between reactive oxygen species (ROS)/antioxidants and mitochondrial function could play a pivotal role in aging physiopathology of viral infections. Here, we correlated plasma oxidants/antioxidants levels elderly admitted to long-term care (LTC) unit with clinical data relation flu-like disease/COVID-19. Moreover, vitro examined effects on cell viability, ROS release function. Materials Methods: In 60 patients LTC for at least 1 year moderate or high load, demographic variables were taken. Blood samples collected evaluations oxidants/antioxidants, as thiobarbituric acid substances, 8-hydroxy-2-deoxyguanosine, 8-isoprostanes, superoxide dismutase activity, glutathione, vitamin D. vitro, human umbilical vascular endothelial cells (HUVEC) used examine membrane potential. Results: The results obtained showed redox state was quite balanced; potential HUVEC reduced by about 20%, only. Also, correlation analysis evidenced association patients' outcomes. Interestingly, lower found who had symptoms suggestive COVID-19 confirmed diagnosis COVID-19. Conclusion: this study highlight importance tendency get syndrome like unit. This information have implications management old population.

Язык: Английский

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Screening the effects of phytoestrogens on lipid metabolism in primary cultured adipocytes from rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata)

Fish Physiology and Biochemistry, Год журнала: 2025, Номер 51(2)

Опубликована: Март 25, 2025

Aquafeed formulation has progressively reduced its dependence on fish-derived ingredients over the past decades. Plant-based substitutes have been a major focus, with soybean meal and derivatives leading way. However, many plants contain phytoestrogens, which may affect fish physiology. This study aimed to assess in vitro effects of genistein (GE), daidzein (DZN), glycitein (GLY), coumestrol (COU) lipid metabolism rainbow trout (Oncorhynchus mykiss) gilthead sea bream (Sparus aurata). Primary cultured adipocytes were incubated these along 17β-estradiol, at two doses each (1, 10, or 100 μM). The μM dose GE DZN decreased adipocyte viability, mainly enhanced accumulation both species, suggesting hypertrophic condition. reduction number content indicated limiting effect adipose tissue growth this species. Interestingly, COU significantly increased cell viability bream, potentially hyperplastic growth, more favorable metabolic state. In that proved be phytoestrogens-sensitive, lipoprotein lipase was generally downregulated upon treatments. Moreover, 10 µM mRNA levels fatty acid transport protein 1 synthase, those binding 1, an acceleration differentiation process compared control cells. work provides new insights into how dietary phytoestrogens modulate supports their presence plant feedstuffs can health production performance.

Язык: Английский

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Precision Nutrition to Activate Thermogenesis as a Complementary Approach to Target Obesity and Associated-Metabolic-Disorders

Cancers, Год журнала: 2021, Номер 13(4), С. 866 - 866

Опубликована: Фев. 18, 2021

Obesity is associated to increased incidence and poorer prognosis in multiple cancers, contributing up 20% of cancer related deaths. These associations are mainly driven by metabolic inflammatory changes the adipose tissue during obesity, which disrupt physiologic homeostasis. The association between obesity hypercholesterolemia, hypertension, cardiovascular disease (CVD) type 2 diabetes mellitus (T2DM) well known. Importantly, retrospective analysis more than 1000 epidemiological studies have also shown positive correlation excess fatness with risk cancer. In addition, important weight, it dysfunctional main driver insulin resistance, syndrome all cause mortality deaths, explains why normal weight individuals may behave as “metabolically unhealthy obese” individuals. Adipocytes direct effects on tumor cells through paracrine signaling. Downregulation adiponectin upregulation leptin serum correlate markers chronic inflammation, crown like structures (CLS) disfunction. Nevertheless, a preventable factor Lifestyle interventions might contribute reduce adverse obesity. Thus, Mediterranean diet interventional been circulation factors, sensitivity function, durable responses years obese patients. supplemented extra-virgin olive oil reduced breast compared control diet. Physical activity another lifestyle systemic biomarkers this scenario, precision nutrition provide complementary approaches target inflammation “unhealthy obesity”. Herein, we first describe different types -thermogenic active brown (BAT) versus energy storing white (WAT). We then move based strategies, mean natural extracts derived from plants and/or ingredients, be useful normalize More specifically, focus two axis: (1) activation thermogenesis BAT browning WAT; (2) potential augmenting oxidative capacity muscles dissipate energy. strategies particularly relevant alleviate immunosuppression, angiogenesis chemotherapy resistance Finally, summarize where plant extracts, mainly, polyphenols flavonoids, applied increase expenditure.

Язык: Английский

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The obesogenic side of Genistein

Frontiers in Endocrinology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 30, 2023

Genistein (GN) has been highly recommended for its medicinal properties like anticancer, antidiabetic, antihyperlipidemic, antiviral, and antioxidant activities among others. Recently, scientists realized that is an endocrine disruptor. It obesogen interferes with the system causing obesity through many mechanisms inducing adipocyte differentiation, lipid accumulation, transformation of some stem cells into adipocytes (bone marrow mesenchymal example) in vitro . Animal studies show GN upregulates genes associated adipogenesis CCAAT/enhancer binding protein alpha (Cebpα), beta (Cebpβ), PPARγ. In silico reveal a strong affinity estrogen receptors. All these findings were contingent on concentration tissues. beyond dispute one most frustrating medical conditions under sun. The pathophysiology this disease was first attributed to high-calorie diet lack physical activity. However, proved two factors are not enough account both children adults. This mini review highlights how interaction peroxisome proliferator-activated receptor gamma can cause obesity.

Язык: Английский

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Aflibercept and Ranibizumab Modulate Retinal Pigment Epithelial Cells Function by Acting on Their Cross Talk with Vascular Endothelial Cells

Cellular Physiology and Biochemistry, Год журнала: 2020, Номер 54(2), С. 161 - 179

Опубликована: Фев. 11, 2020

We performed co-culture experiments between human RPE cells (ARPE-19) and umbilical vascular endothelial (HUVEC) in order to evaluate how anti-VEGF drugs could affect NO release, mitochondrial function, the oxidative status, proliferation migration of through modulation their cross talk with cells.The HUVEC/RPE, was exposed Ranibizumab/Aflibercept absence/presence synthase (NOS) inhibitor, phosphatidylinositol 3'-kinase (PI3K), extracellular-signal-regulated kinases 1/2 (ERK1/2) p38 mitogen-activated protein kinase (p38 MAPK) blockers. Specific kits were used for cell viability, membrane potential, NO, ROS GSH production. Western blot apoptosis markers, NOS isoforms, others detection. Cell analyzed by scratch assay, whereas cycle xCELLigence flow cytometry.In co-cultured HUVEC physiological conditions, Aflibercept/Ranibizumab increased release a dose time-dependent way. Opposite results obtained peroxidative conditions. Both agents able prevent fall viability an effect which reduced various inhibitors, migration. counteracted changes expression/activation, PI3K ERK1/2 activation caused peroxidation. These confirmed analysis.This study has shown new mechanisms at basis protective effects elicited cells. stimulated Aflibercept/Ranibizumab, some paracrine factors that can modulate response both physiologic

Язык: Английский

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Dietary supplementation with Aloe vera induces hepatic steatosis and oxidative stress together with a disruption of cellular signaling pathways and lipid metabolism related genes' expression in gilthead sea bream (Sparus aurata)

Aquaculture, Год журнала: 2022, Номер 559, С. 738433 - 738433

Опубликована: Июнь 2, 2022

Язык: Английский

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Exposure to Plasma From Non-alcoholic Fatty Liver Disease Patients Affects Hepatocyte Viability, Generates Mitochondrial Dysfunction, and Modulates Pathways Involved in Fat Accumulation and Inflammation

Frontiers in Medicine, Год журнала: 2021, Номер 8

Опубликована: Июль 2, 2021

Changes of lipidic storage, oxidative stress and mitochondrial dysfunction may be involved in the pathogenesis non-alcoholic fatty liver disease (NAFLD). Although knowledge intracellular pathways has vastly expanded recent years, role mechanisms circulating triggering factor(s) are debated. Thus, we tested hypothesis that factors blood NAFLD patients influence processes underlying disease. Huh7.5 cells/primary human hepatocytes were exposed to plasma from 12 healthy subjects specific assays performed examine viability, H 2 O reactive oxygen species (ROS) release, membrane potential triglycerides content. The involvement NLRP3 inflammasome signaling related peroxisome-proliferator-activating-ligand-receptor-γ (PPARγ), sterol-regulatory-element-binding-protein-1c (SREBP-1c), nuclear-factor-kappa-light-chain-enhancer activated B cells (NF-kB), NADPH oxidase (NOX2) was evaluated by repeating experiments presence blocker, MCC950, through Western blot. results obtained shown able reduce cell viability about 48 24% ( p < 0.05), increase , ROS, content 42, 19, 16% respectively. An increased expression SREBP-1c, PPARγ, NF-kB NOX2 51, 121, 63, 46%, respectively, observed as well. Those effects reduced use MCC950. hepatocytes, exposure induces a NAFLD-like phenotype interference with NLRP3-inflammasome activation NOX2.

Язык: Английский

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Recent developments in natural products for white adipose tissue browning

Chinese Journal of Natural Medicines, Год журнала: 2020, Номер 18(11), С. 803 - 817

Опубликована: Ноя. 1, 2020

Язык: Английский

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