Food Science & Nutrition,
Год журнала:
2024,
Номер
12(7), С. 5077 - 5086
Опубликована: Апрель 4, 2024
(GC)
is
a
red
algal
species
that
primarily
consumed
in
Asia.
Here,
we
investigated
the
effect
of
GC
on
obesity-related
skeletal
muscle
wasting.
Furthermore,
elucidating
its
impact
activation
sirtuin
1
(SIRT1)/peroxisome
proliferator-activated
receptor
gamma
coactivator
1α
(PGC1α)
constituted
critical
aspect
understanding
underlying
mechanism
action.
In
this
study,
6-week-old
male
C57BL/6
mice
were
fed
high-fat
diet
(HFD)
for
8
weeks
to
induce
obesity,
then
continued
HFD
another
while
orally
administered
GC.
decreased
ectopic
fat
accumulation
and
increased
weight,
size,
function
obese
mice.
reduced
atrophy
hypertrophy
We
hypothesized
SIRT1/PGC1α
by
regulates
hypertrophy.
observed
expression
SIRT1
PGC1α
C2C12
cells,
which
mitochondrial
biogenesis.
addition,
when
cells
treated
with
SIRT1-specific
inhibitor
EX-527,
no
changes
protein
levels
GC-treated
cells.
Therefore,
attenuated
wasting
improving
biogenesis
through
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Ноя. 11, 2024
Human
melanoma
is
linked
with
aging-related
disorders,
prompting
interest
in
the
development
of
functional
foods
derived
from
natural
ingredients
to
mitigate
its
incidence.
Molecules
green
seaweeds
such
as
Caulerpa
racemosa
can
serve
this
purpose
due
their
anti-tumor
and
anti-inflammatory
properties.
A
previous
work
study
compounds
profiling
has
been
carried
out,
research
molecular
docking
studies
targeting
receptors
associated
(GRP78,
IRE1,
BRAF)
aging
(mTOR,
AMPK,
SIRT1)
identified
four
promising
compound
an
extract
C.
racemosa.
The
current
aims
mechanism
those
at
a
cellular
level
using
human
A375
(BRAF-V600E
mutation)
B16-F10
cell
lines.
MTT
assay
was
used
evaluate
potential
GSCRE
against
lines,
comparisons
made
normal
HDFa
Results
indicated
that
C2,
also
known
Caulersin,
demonstrated
significantly
different
∆G
affinity
binding
score
compared
control
drug
Dabrafenib.
crude
extract,
particularly
showed
modulating
mTOR,
SIRT1
pathways
downregulating
GRP78,
BRAF
signaling
(p
<
0.05).
Interestingly,
C2
less
effective
suppressing
lines
(LD
