Sialyllactose Enhances the Short-Chain Fatty Acid Production and Barrier Function of Gut Epithelial Cells via Nonbifidogenic Modification of the Fecal Microbiome in Human Adults

Microorganisms, Год журнала: 2024, Номер 12(2), С. 252 - 252

Опубликована: Янв. 25, 2024

Although various benefits of human milk oligosaccharides (HMOs) have been reported, such as promoting Bifidobacterium growth in the infant gut, their effects on adults not fully studied. This study investigated two types sialyllactose, 3′-sialyllactose (3′-SL) and 6′-sialyllactose (6′-SL), adult intestinal microbiome using simulator microbial ecosystem (SHIME®), which can simulate gastrointestinal conditions. HPLC metabolite analysis showed that sialyllactose (SL) supplementation increased short-chain fatty acid content SHIME culture broth. Moreover, 16S rRNA gene sequencing revealed SL promoted Phascolarctobacterium Lachnospiraceae, acid-producing bacteria, but Bifidobacterium. Altogether, both stimulated an increase acids, including propionate butyrate. Additionally, supernatant supplemented with improved barrier function Caco-2 cell monolayers. These results suggest could act a unique prebiotic among other HMOs nonbifidogenic effect, resulting protection.

Язык: Английский

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Research Progress on the Degradation of Human Milk Oligosaccharides (HMOs) by Bifidobacteria

Nutrients, Год журнала: 2025, Номер 17(3), С. 519 - 519

Опубликована: Янв. 30, 2025

The purpose of this study was to investigate the degradation mechanism Bifidobacterium on breast milk oligosaccharides (HMOs) and its application in infant nutrition. composition characteristics HMOs were introduced, by described, including intracellular extracellular digestion species-specific differences. interaction between Bacteroides process degrading effect intestinal microecology analyzed. effects HMO formula powder microbiota infants discussed, simulating composition, regulating flora immune function, infection prevention, brain development. Finally, research results are summarized, future directions proposed provide for field

Язык: Английский

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Multi-level metabolic engineering of 6ʹ-sialyllactose in Escherichia coli

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106078 - 106078

Опубликована: Фев. 1, 2025

Язык: Английский

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Microbial micronutrient sharing, gut redox balance and keystone taxa as a basis for a new perspective to solutions targeting health from the gut

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Март 16, 2025

In health, the gut microbiome functions as a stable ecosystem maintaining overall balance and ensuring its own survival against environmental stressors through complex microbial interaction. This protection from is maintained interactions both within bacterial well with host. As consequence, plays critical role in various physiological processes including structure function of barrier, educating immune system, modulating motor, digestive/absorptive, neuroendocrine system all which are crucial for human health disease pathogenesis. Pre- probiotics, widely available clinically established, offer benefits primarily by beneficially microbiome. However, their clinical outcomes can vary significantly due to differences host physiology, diets, individual compositions, other factors. perspective paper highlights emerging scientific insights into importance micronutrient sharing, redox balance, keystone species, barrier diverse functional ecosystem, relevance health. We propose novel approach that targets ecosystems taxa performance supplying micronutrients form colon-delivered vitamins, precision prebiotics [e.g. milk oligosaccharides (HMOs) or synthetic glycans] components precisely tailored ingredient combinations optimize Such strategy may effectively support stabilize ecosystems, providing more robust consistent across individuals conditions, thus, overcoming limitations current single biotic solutions.

Язык: Английский

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Investigating Plasma Metabolomics and Gut Microbiota Changes Associated With Parkinson Disease

Neurology, Год журнала: 2025, Номер 104(10)

Опубликована: Апрель 24, 2025

Coffee intake is linked to a reduced risk of Parkinson disease (PD), but whether this effect mediated by gut microbiota and metabolomic changes remains unclear. This study examines PD-associated shifts, caffeine metabolism, their connection microbiome alterations in multicenter study. We conducted an untargeted serum assay using liquid chromatography with high-resolution mass spectrometry on exploratory cohort recruited from National Taiwan University Hospital (NTUH). A targeted focusing its 12 downstream metabolites was validated independent Malaya Medical Centre (UMMC). In the cohort, association each metabolite investigated metagenomic shotgun sequencing. clustering-based approach used correlate plasma level clinical severity. Body index, antiparkinsonism medication use, dietary habits (including coffee tea intake) were recorded. Sixty-three patients PD 54 controls NTUH formed while 36 20 UMMC served as validation replicate findings. total 5,158 detected analysis, 3,131 having high confidence for analysis. Compared controls, abundance 56 significantly higher that 7 lower (adjusted p < 0.05 log2 fold change >1) PD. Caffeine metabolism (p = 0.0013), levels negatively correlated motor severity 0.01). Targeted analysis confirmed metabolites, including theophylline, paraxanthine, 1,7-dimethyluric acid, 5-acetylamino-6-amino-3-methyluracil, PD; these findings replicated 0.05). clustering found species enriched paraxanthine theophylline (both 0.05), notably Clostridium sp000435655, Acetatifactor sp900066565, Oliverpabstia intestinalis, Ruminiclostridium siraeum. identifies PD-related microbial-caffeine compared controls. Our offer insights future functional research caffeine-microbiome interactions

Язык: Английский

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2'-Fucosyllactose restores the intestinal mucosal barrier in ulcerative colitis by inhibiting STAT3 palmitoylation and phosphorylation

Clinical Nutrition, Год журнала: 2023, Номер 43(2), С. 380 - 394

Опубликована: Дек. 20, 2023

Язык: Английский

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2′-Fucosyllactose and 3′-Sialyllactose Reduce Mortality in Neonatal Enteroaggregative Escherichia coli Infection by Improving the Construction of Intestinal Mucosal Immunity

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(47), С. 26165 - 26177

Опубликована: Ноя. 13, 2024

Human milk oligosaccharides could prevent pathogenic bacterial infections in neonates; however, direct vivo anti-infection evidence was still lacking. Here, we systematically evaluated the effects of 2'-fucosyllactose (2'-FL) and 3'-sialyllactose (3'-SL) on structural development functional maturation neonates their defense against enteroaggregative

Язык: Английский

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Human milk oligosaccharides combine with Bifidobacterium longum to form the “golden shield” of the infant intestine: metabolic strategies, health effects, and mechanisms of action

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Ноя. 21, 2024

Human milk oligosaccharides (HMOs) are the third most important nutrient in human and gold standard for infant nutrition. Due to lack of an enzyme system capable utilizing HMOs intestine, cannot be directly utilized. Instead, they function as natural prebiotics, participating establishment intestinal microbiota a "bifidus factor." A crucial colonizer early intestine is

Язык: Английский

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De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis

Metabolic Engineering, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

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The effect of non-genetic determinants of human milk oligosaccharide profiles in milk of Ugandan mothers

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 22, 2024

Abstract Background Human milk oligosaccharides (HMOs) protect against infection and promote growth cognitive development in breastfeeding children. Non-genetic factors which influence HMO composition mothers rural Africa have not been investigated. Objective We undertook a cross-sectional study to determine the association between profiles non-genetic maternal children’s sex Ugandan mother-children pairs. Method was collected from 127 by manual expression. analysis high performance liquid chromatography. The proportion of each per total concentration calculated. Spearman’s correlation Mann-Whitney U test were used assess relationship individual HMOs infant sex. Result Nineteen assayed. prevalence secretor non-secretor status, based on with concentrations 2’FL LNFP 1, 80.3 % 19.7 %, respectively. In mothers, 2’FL, DFLac I constituted > 57 while LNT LNFPII 46.9 measured HMOs. median 3’SL all male children significantly higher than that female multiparous compared primiparous mothers. Higher FDSLNH lower LNH observed overweight Median DSLNT < 18 years old old. Concentrations specific increased, decreased, or remained unchanged increasing lactation duration Conclusions Specific associated age, parity post-partum BMI but different those reported other populations.

Язык: Английский

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