Hypolipidemic activity of phytochemical combinations: A mechanistic review of preclinical and clinical studies

Food Chemistry, Год журнала: 2024, Номер 459, С. 140264 - 140264

Опубликована: Июль 3, 2024

Язык: Английский

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Lupeol protect against LPS-induced neuroinflammation and amyloid beta in adult mouse hippocampus

Frontiers in Nutrition, Год журнала: 2024, Номер 11

Опубликована: Июль 10, 2024

Neuroinflammation includes the activation of immune glial cells in central nervous system, release pro-inflammatory cytokines, which disrupt normal neural function and contribute to various neurological disorders, including Alzheimer's disease (AD), Parkinson's disease, multiple sclerosis, stroke. AD is characterized by factors amyloidogenesis, synaptic dysfunction, memory impairment neuroinflammation. Lipopolysaccharide (LPS) constitutes a vital element membrane gram-negative bacterial cell, triggering vigorous neuroinflammation facilitating neurodegeneration. Lupeol, naturally occurring pentacyclic triterpene, has demonstrated several pharmacological properties, notably its anti-inflammatory activity. In this study, we evaluated anti-Alzheimer activity lupeol lipopolysaccharide (LPS)-injected mice model. LPS (250ug/kg) was administered intraperitoneally C57BL/6 N male for 1 week induce cognitive impairment. For biochemical analysis, acetylcholinesterase (AChE) assay, western blotting confocal microscopy were performed. AChE, blot immunofluorescence results showed that treatment (50 mg/kg) along with administration significantly inhibited LPS-induced neuroinflammatory mediators cytokines like nuclear factor (NF-κB), tumor necrosis (TNF-α), cyclooxygenase (COX-2) interleukin (IL-1β). Furthermore, found systemic inflammation lead symptoms as enhances level amyloid beta (Aβ), precursor protein (APP), Beta-site APP cleaving enzyme (BACE-1) hyperphosphorylated Tau (p-Tau). Lupeol reversed elevated Aβ, APP, BACE-1 p-Tau hippocampus, showing anti-Alzheimer's properties. It also determined prevented dysfunction via enhanced expression pre-and post-synaptic markers SNAP-23, synaptophysin PSD-95. Overall, our study shows prevents inhibition processes. Hence, suggest might be useful therapeutic agent prevention neuroinflammation-induced disorders AD.

Язык: Английский

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Ecofriendly fabrication of natural jojoba nanoemulsion and chitosan/jojoba nanoemulsion with studying the antimicrobial, anti-biofilm, and anti-diabetic activities in vitro

Biomass Conversion and Biorefinery, Год журнала: 2023, Номер unknown

Опубликована: Дек. 16, 2023

Язык: Английский

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Overview Of a Novel Osmotin Abolishes Abnormal Metabolic-Associated Adiponectin Mechanism in Alzheimer’s disease: Peripheral and CNS Insights

Ageing Research Reviews, Год журнала: 2024, Номер 100, С. 102447 - 102447

Опубликована: Авг. 5, 2024

Язык: Английский

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Lupeol Attenuates Palmitate-Induced Hypertrophy in 3T3-L1 Adipocytes

Biomolecules, Год журнала: 2025, Номер 15(1), С. 129 - 129

Опубликована: Янв. 15, 2025

Obesity is characterized by the enlargement of adipose tissue due to an increased calorie intake exceeding body’s energy expenditure. Changes in size can lead harmful consequences, with excessive fat accumulation resulting adipocyte hypertrophy and promoting metabolic dysfunction. These adiposity-associated pathologies be influenced dietary components their potential health benefits. Lupeol, a pharmacologically active pentacyclic triterpenoid found medicinal plants, vegetables, fruits, has been shown exhibit antioxidant anti-inflammatory properties. This study investigated role lupeol on evaluating key adipogenic regulators vitro. First, 3T3-L1 MBX mouse embryonic cells were differentiated into adipocytes was induced using 500 µM palmitic acid. The treated showed significantly lipid droplet size, confirming hypertrophy. Both hypertrophied then or without 60 lupeol, following dose-dependent study. Lipid assessed validated Oil Red O staining. Western blot analysis performed measure expression inflammatory markers. Differentiated fatty acid-binding protein 4 (FABP4) staining, indicating content. revealed that treatment reduced FABP4, peroxisome proliferator-activated receptor-γ (PPARγ), adipokines. In conclusion, results suggest reverts markers are enhanced Through its effects, offers protective effects against contributes reducing hypertrophic adiposity.

Язык: Английский

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Reduction in presynaptic glutamate release and the prevention of glutamate excitotoxicity by lupeol in rats

Neurochemistry International, Год журнала: 2025, Номер unknown, С. 105951 - 105951

Опубликована: Фев. 1, 2025

Язык: Английский

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Triterpenes as promising antiprostate cancer agents

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 197 - 219

Опубликована: Янв. 1, 2025

Язык: Английский

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Chemical Analysis and Antioxidant Activities of Resin Fractions from Pistacia lentiscus L. var. Chia in Neuroblastoma SH-SY5Y Cells

Molecules, Год журнала: 2025, Номер 30(5), С. 997 - 997

Опубликована: Фев. 21, 2025

Chios mastiha is the natural aromatic resin of Pistacia lentiscus L. var. Chia, Anacardiaceae, which exclusively cultivated in southern part Greek island Chios. (P. lenticonus/Chios mastiha) well-known for its distinctive taste and aroma has been known since ancient times due to healing properties gastrointestinal inflammatory disorders because anti-bacterial anti-fungal activities. In this study, chemical composition, applying LC-QTOF-MS/MS analysis, antioxidant activities three different polarity P. fractions, apolar, medium polar, were characterized human neuroblastoma SH-SY5Y cells. Chemical analysis fractions unveiled new components mastiha, mainly fatty acids compounds, their activity regulatory effects on lipid metabolism. By MTT assay confocal microscopy we showed that especially apolar polar enriched triterpenes acids, caused suppression H2O2-induced reduction cell viability, ROS production, depolarization mitochondrial membrane potential, Moreover, Western blot revealed fraction, induced expression PPARα, crucial role Induction a GR target gene, was also accompanied by an increase protein levels. Enhanced fraction may be correlated with triterpenoids. Thus, our results indicate neuroprotective actions highlighting potential application as agents neurodegenerative diseases.

Язык: Английский

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Chemical composition and biological activity of lemongrass volatile oil and n-Hexane extract: GC/MS analysis, in vitro and molecular modelling studies

PLoS ONE, Год журнала: 2025, Номер 20(2), С. e0319147 - e0319147

Опубликована: Фев. 25, 2025

Lemon grass, formally identified as Cymbopogon citratus , is a plant that belongs to the Poaceae family. The present work aimed examine chemical composition by GC/MS analysis and assess biological potential of C. volatile oil n -hexane extract. extract were evaluated for antioxidant tested their enzyme inhibition against tyrosinase, butyrylcholinesterase (BChE), acetylcholinesterase (AChE), α -amylase, -glucosidase. lemongrass n-hexane (HE) (VO) revealed main constituents in HE are aliphatic hydrocarbons (42.98%), triterpenoids (20.14%), aromatic (17.25%). Conversely, predominantly monoterpenes, namely α-citral (36.08%), β -citral (34.22%), -myrcene (13.84%). showed more potent DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum (10.18, 35.69 mg Trolox equivalent/g, 98.97 69.73 equivalent/g 43.01 mmol equivalent/g). displayed higher BChE (1.53 Galanthamine equivalent)/g), well -amylase -glucosidase inhibitory activities (0.39 2.40 Acarbose VO demonstrated tyrosinase (57.19 Kojic acid equivalent/g) along with acetyl inhibition. Dominant compounds exhibited ability bind high affinity various target proteins, particular AChE BChE. show significant promise viable choice advancement novel therapeutic strategies at addressing oxidative stress, neurodegeneration, diabetes.

Язык: Английский

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Antimicrobial and antiparasitic potential of lupeol: antifungal effect on the Candida parapsilosis species complex and nematicidal activity against Caenorhabditis elegans

Journal of Medical Microbiology, Год журнала: 2025, Номер 74(3)

Опубликована: Март 7, 2025

Introduction. There is growing concern about infections caused by non-albicans Candida species, including species of the parapsilosis complex - which have seen a considerable increase in cases during COVID-19 pandemic addition to nematode resistance currently used anthelmintics.Gap Statement. Lupeol triterpenoid phytosterol that has wide range biological activities, although its antifungal and antiparasitic potential still poorly explored. Additionally, effect on biofilm C. not yet been studied.Aim. This study aimed investigate lupeol against planktonic cells mature biofilms, as well nematicidal Caenorhabditis elegans. In addition, molecular docking was performed identify target molecules for lupeol's effect.Methodology. Twelve strains were used. Planktonic susceptibility through broth microdilution assay, while antibiofilm investigated measuring biomass metabolic activity. The mechanism action fishing. evaluation using elegans infection model.Results. demonstrated activity with MIC between 64 512 µg ml-1. able reduce starting from concentration 1024 ml-1 It observed there interaction enzyme 14α-demethylase. Furthermore, had ml-1.Conclusion. exhibits an complex, forms, possibly affecting ergosterol synthesis. further potential.

Язык: Английский

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