Exploring the causal relationship between inflammatory cytokines, metabolites, and Behcet's syndrome: Mendelian randomization

Cytokine, Год журнала: 2025, Номер 186, С. 156849 - 156849

Опубликована: Янв. 5, 2025

Язык: Английский

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Association Between Circulating Fatty Acids and Blood Pressure: A Review

Current Nutrition Reports, Год журнала: 2025, Номер 14(1)

Опубликована: Янв. 8, 2025

Язык: Английский

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The potential of three endemic Alocasia species in Indonesia: Insights from advancing in vitro propagation methods and comparative metabolites analysis

South African Journal of Botany, Год журнала: 2025, Номер 177, С. 713 - 728

Опубликована: Янв. 13, 2025

Язык: Английский

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Effects of different Lys/Met ratios on the antioxidant capacity, tissue morphology, and fatty acid composition of subcutaneous fat in Tibetan sheep on low-protein diets: a lipidomic analysis

Frontiers in Veterinary Science, Год журнала: 2025, Номер 11

Опубликована: Янв. 30, 2025

Introduction This study employed lipidomics to investigate the effects of varying lysine (Lys)- to-methionine (Met) ratios on antioxidant capacity, tissue morphology, and fatty acid composition subcutaneous fat in Tibetan sheep fed a low-protein diet. Methods Ninety healthy male similar body weight were randomly allocated into three groups. These diet containing Lys/Met 1:1, 2:1, 3:1. Ultra-High Performance Liquid Chromatography–tandem Mass Spectrometry (UHPLC–MS/MS) was explore changes various lipid subclasses adipose tissue. The expression genes associated with adipogenesis, metabolism also examined. Results results indicated that 1:1 group exhibited significantly higher capacity (glutathione peroxidase, GSH-Px), more orderly adipocyte arrangement, uniform cell size, general increase unsaturated levels. Additionally, several molecules phenotype (Antioxidant index content) identified, namely, DG(38:3e) + Na, PE(17:1_22:2)-H, PI(17:0_20:3)-H, TG(33:0e) NH4, Cer(d14:0_17:1) H, CL(81:13)-2H. Furthermore, findings showed upregulation PPARγ, FASN, FAD4, CPT1A , GPX4 can enhance differentiation accumulation, thereby improving metabolic function via regulation oxidative defense mechanisms. Discussion In summary, this provides theoretical foundation for optimizing precision feeding strategies sheep, offering crucial data support enhancements production efficiency meat quality.

Язык: Английский

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Healthy Aging Metabolomic and Proteomic Signatures Across Multiple Physiological Compartments

Aging Cell, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

ABSTRACT The study of biomarkers in biofluids and tissues expanded our understanding the biological processes that drive physiological functional manifestations aging. However, most these studies were limited to examining one compartment, an approach fails recognize aging pervasively affects whole body. simultaneous modeling hundreds metabolites proteins across multiple compartments may provide a more detailed picture healthy point differences between chronological Herein, we report proteomic analyses plasma urine collected men women, age 22–92 years. Using data, developed series metabolomic predictors for plasma, urine, skeletal muscle. We then defined score, which measures departure individual's predicted expected individual based on full cohort. show are significantly independently related clinical phenotypes important aging, such as inflammation, iron deficiency anemia, muscle mass, renal hepatic functions. Despite different set selected each scores reflect similar degree deviation from single individuals, thus allowing identification subjects with significant accelerated or decelerated

Язык: Английский

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Impact of glucose and propionic acid on even and odd chain fatty acid profiles of oleaginous yeasts

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 18, 2025

Abstract Background Odd chain fatty acids (OCFAs) are gaining attention for their valuable medical and nutritional applications. Microbial fermentation offers a sustainable environmentally friendly alternative OCFA production compared to traditional extraction or chemical synthesis methods. To achieve an economically feasible process, it is essential identify develop microbial cell factories capable of producing OCFAs with high titers yields. Results We selected 19 yeast species, including both oleaginous yeasts representatives from the Ascomycota Basidiomycota phyla, based on known potential ability produce OCFAs. These species were screened under various growth conditions evaluate potential. In glucose-based, nitrogen-limited media, strains produced varying extents, comprising 0.5–5% total acids. When using precursor propionic acid as sole carbon source, only eight exhibited growth, tolerance concentrations between 5 29 g/L. The also displayed efficiencies in converting into acids, yielding 0.16 1.22 g/L constituting 37–89% Among top performing strains, Cutaneotrichosporon oleaginosus highest yields (0.94 g/L, 0.07 g/g), Yarrowia lipolytica demonstrated superior rates even at elevated concentrations, Rhodotorula toruloides achieved proportion relative (89%). Conclusions Our findings highlight diverse capacities production, identifying several promising further optimization processes. Graphical

Язык: Английский

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The effects of immune cell phenotypes and plasma metabolomes on diabetic foot ulcer: a Mendelian randomization study and mediation analysis

Archives of Dermatological Research, Год журнала: 2025, Номер 317(1)

Опубликована: Фев. 22, 2025

Язык: Английский

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HPTLC hyphenated with planar effect-directed assays (EDA) for the rapid screening and evaluation of macro algae fermentation with respect to their antioxidant and anti-inflammatory activity

Algal Research, Год журнала: 2025, Номер unknown, С. 103978 - 103978

Опубликована: Фев. 1, 2025

Язык: Английский

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Enhancing the Accuracy of Identification and Relative Quantification of Unsaturated Fatty Acids in Serum via a Stable Isotope-Labeled Double Derivatization Strategy

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Accurate identification and quantification of fatty acids are critical for investigating their biological function in disease models. Although several derivatization methods have been proposed identifying the positions C═C bonds unsaturated acids, poor ionization efficiency carboxyl group leads to lower intensity molecular ion peaks, making difficult interfering with accuracy based on peak areas characteristic pairs. In this study, a strategy stable isotope-labeled combined was employed simultaneously using d0/d9-5-amino-N,N,N-trimethylpentane-1-ammonium iodide (d0/d9-ATPAI) label m-chloroperoxybenzoic acid bonds. The quaternary amine groups novel reagent d0/d9-ATPAI can enhance recognition pairs facilitate structural elucidation various acids. heavy be served as internal standards achieve accurate relative position isomers individual among samples area ratio Unsaturated positional were quantified aldehyde or alkenyl diagnostic ions. addition, saturated m/z 86.09679 cyclamine ion. This approach enhanced detection sensitivity by 60,000 times, allowing characterization 70 rat serum, including 26 isomers. Pseudotargeted metabolomics analysis serum revealed alterations metabolic pathway during diabetic cognitive dysfunction. Overall, method, high wide coverage, could provide complex matrices.

Язык: Английский

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Cultivation technology optimization and identification of secondary metabolites from elm oyster mushroom Hypsizygus ulmarius (Bull.) Redhead (Agaricomycetes) through GC-MS metabolomic profiling from India

Mycologia, Год журнала: 2025, Номер unknown, С. 1 - 27

Опубликована: Март 20, 2025

Hypsizygus ulmarius, a novel oyster mushroom species, offers potential scope due to its low-cost production, high biological efficiency, and notable nutritional, medicinal, therapeutic properties. The present study standardized commercial cultivation technology for H. ulmarius in the Trans-Gangetic Plains of India. mycelium grew well on potato dextrose agar carrot extract broth at pH 8.0 temperature 25 C. Wheat straw supplemented with cotton seed hull rate 10.0% dose, spawned pearl millet grain spawn 5.0% gave highest yield (953.66 g/0.4 kg dry substrate) efficiency (238.41%). first flush sporocarps were freeze-dried physicochemical characterization, revealing 16 strong peaks 8 functional groups via Fourier transform infrared (FTIR) analysis. Particle size averaged 45.97 µm powder, scanning electron microscopy (SEM) analysis showed diverse surface textures. Gas chromatography-mass spectrometry metabolic profiling identified 20 key secondary metabolites each from hexane methanolic extracts, uses that are valuable pharmaceutical, nutraceutical, food industry applications.

Язык: Английский

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