Research on the protective effect of Rhizoma of Anemarrhena asphodeloides on TMT induced AD mice model based on network pharmacology combined with in vitro and in vivo experimental validation

Journal of Pharmacological Sciences, Год журнала: 2024, Номер 157(2), С. 75 - 87

Опубликована: Дек. 25, 2024

Alzheimer's disease (AD) is a neurodegenerative disease, and neuroprotection an important approach to improving AD outcomes. Rhizoma of Anemarrhena asphodeloides (RAA) commonly used Traditional Chinese Medicine (TCM) with demonstrated neuroprotective effects, but its anti-AD mechanism requires further exploration. To elucidate the RAA on TMT-induced mice. The mice model was established via intraperitoneal injection TMT. effect ameliorating learning memory assessed using Morris Water Maze (MWM) Y-maze tests. Haematoxylin-Eosin (HE), Nissl, TUNEL staining were observe RAA. components in serum containing (RAA-S) detected UPLC-Q-Orbitrap MS. Potential targets predicted through network pharmacology molecular docking. Serum levels reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), peroxidase (GPx) measured ELISA kits. HT22 hippocampal neuronal cell line injured by l-glutamate (L-Glu) ROS cells 2'-7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe flow cytometry. Apoptosis proteins MAP2, GAP-43, Nrf2, Keap1, HO-1, Bax, Bcl-2 Western blotting. Immunofluorescence employed Nrf2 nuclear translocation. significantly increased residence time W zone enhanced correct alternation rate TMT-treated preserved integrity orderly arrangement nerve cells. A total 12 RAA-S. AKT1, PPARG, CASP3, STAT3, HSP90AA1, NFE2L2 (Nrf2) involved RAA-S target pathway network. Molecular docking revealed that exhibited highest average binding energy all In vivo, elevated HO-1 levels, along GPx, GSH, SOD activity, which had been reduced Additionally, MDA ROS, vitro, apoptosis accumulation caused Furthermore, promoted expression N-Nrf2 L-Glu-injured attenuated oxidative stress induced TMT L-Glu underlying associated activation Nrf2/Keap1-HO-1 pathway.

Язык: Английский

Assessment of Neuromodulatory Effects of Origanum punonense Danin Essential Oil on AMPA Receptor Function Using Whole-Cell Patch-Clamp Technique

European Journal of Integrative Medicine, Год журнала: 2024, Номер unknown, С. 102411 - 102411

Опубликована: Окт. 1, 2024

Язык: Английский