The Protective Role of Folic Acid in Biochemical and Histopathological Changes Induced by Azithromycin in the Livers of Pregnant Albino Rats

Medicina, Год журнала: 2025, Номер 61(3), С. 415 - 415

Опубликована: Фев. 27, 2025

Background and Objectives: We evaluated the impact of second-generation macrolide azithromycin on pregnant albino rats’ livers assessed protective role folic acid. Materials Methods: For this study, forty rats were treated then examined. Forty divided into four equal groups (I–IV). In Group I, each rat was gavaged with 1 mL distilled water day 16 pregnancy, 0.5 from pregnancy to 20. II, 0.45 containing 9 mcg acid 17 III, mg 16th day, 4.5 IV, At end experiments, blood samples studied subjected biochemical, histological, morphometric analysis. Results: Azithromycin induced pathological changes in livers, as evidenced by disturbed lobular architecture, cytoplasmic vacuoles, deeply stained hepatic nuclei, elevated liver enzymes. The co-administration ameliorated most changes. Conclusions: caused alterations rats, co-supplementation is encouraged, minimize these

Язык: Английский

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Cytopenias in Autoimmune Liver Diseases—A Review

Journal of Clinical Medicine, Год журнала: 2025, Номер 14(5), С. 1732 - 1732

Опубликована: Март 4, 2025

Autoimmune liver diseases (AiLDs), including autoimmune hepatitis (AIH), primary biliary cholangitis (PBC), and sclerosing (PSC), are immune-mediated conditions associated with significant hepatic systemic manifestations. Among these, cytopenias—defined as reductions in blood cell counts affecting single or multiple lineages—represent a clinically important, though often under-recognized, complication. Cytopenias AiLDs arise from diverse mechanisms, destruction, hypersplenism due to portal hypertension, bone marrow suppression, nutritional deficiencies. These abnormalities can exacerbate bleeding, infections, fatigue, complicating the disease course impacting therapeutic strategies. Immune-mediated cytopenias, such hemolytic anemia (AIHA), immune thrombocytopenic purpura (ITP), neutropenia (AIN), more frequently AIH, whereas cytopenias PBC PSC largely attributed hypersplenism. Diagnostic evaluation involves systematic approach combining clinical history, laboratory testing (e.g., complete counts, Coombs tests, assessments), imaging studies, complex cases. Treatment strategies aim address underlying cause of immunosuppressive therapy for beta-blockers splenectomy hypersplenism, supplementation Challenges include distinguishing between immune- hypersplenism-related managing drug-induced optimizing care transplant candidates. The recently recognized IgG4-related disease, mimicking cholestatic AiLDs, adds another layer complexity, given its association This review aims act guide clinician dealing patients respect occurrence specific focus on pathophysiology management these cytopenias. Furthermore, there need be enhanced multidisciplinary discussions about those hematologists hepatologists, maintenance high index suspicion rarer causes part treating physician, is further studies elucidate mechanisms behind AiLDs.

Язык: Английский

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Folic acid intervention ameliorates hepatic steatosis after long-term alcohol exposure by alleviating endoplasmic reticulum stress

The Journal of Nutritional Biochemistry, Год журнала: 2025, Номер unknown, С. 109896 - 109896

Опубликована: Март 1, 2025

Язык: Английский

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Elevated red blood cell folate levels are associated with metabolic dysfunction-associated steatotic liver disease: results from NHANES 2017–2020

Frontiers in Physiology, Год журнала: 2025, Номер 16

Опубликована: Март 20, 2025

Introduction Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most prevalent chronic worldwide. However, role of folate in MASLD remains controversial. This study aimed to investigate association between two indicators [serum and red blood cell (RBC) folate] prevalence using data from 2017–2020 National Health Nutrition Examination Survey (NHANES). Methods A total 3,879 participants without or significant alcohol consumption were included final analysis. Hepatic steatosis was assessed via transient elastography, with defined as a controlled attenuation parameter (CAP) ≥285 dB/m presence at least one cardiometabolic risk factor. Logistic regression generalized additive models (GAMs) used evaluate associations levels MASLD, subgroup analyses stratified by age, gender, body mass index (BMI). Results After full adjustment for confounders, RBC exhibited positive (OR = 1.111 95% CI: 1.015–1.216 per 1-unit increase). In contrast, serum showed negative minimally adjusted 0.869 0.802–0.941), which disappeared after further adjustments. Subgroup confirmed that BMI did not modify folate–MASLD relationship. Discussion These findings suggest elevated are independently associated prevalence, whereas may lack clinical relevance due susceptibility confounding factors. folate, stable biomarker long-term status, serve superior indicator investigating associations.

Язык: Английский

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Severe Hyperhomocysteinemia, an Unusual Case of Liver Cirrhosis

Digestive Diseases and Sciences, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Язык: Английский

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Folic Acid-Targeted Liposome-Based Nanoparticle Loaded with Sorafenib for Liver Cancer Therapy

International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 3933 - 3944

Опубликована: Март 1, 2025

Sorafenib (SF) is a small molecule involved in tumor proliferation and angiogenesis. SF inhibitor of several kinases, including RAF, VEGFR, PDGFR. However the weak targeting ability for liver tissues major problem clinical therapy. Therefore, SF-loaded folic acid-targeted liposome drug delivery system was devised therapy this study. Folic acid (FA), HSPC, DSPE-PEG2k, CHO, were composed to prepare (LSF) system. LSF loading content established through thin-film-hydration technique HPLC, respectively. The particle size stability examined by dynamic light scattering (DLS). inhibition effect elucidated vitro on cancer cells cell cytotoxicity apoptosis experiments. tumor-inhibiting efficacy measured xenograft model. (DLC) 3.6%. diameter 197.1±16.6 nm, stable during 24 h. Liver could be effectively inhibited vitro. substantially induce apoptosis. Also, inhibit growth vivo. reduce side effects demonstrated bio-safety tests. FA-targeted that progression cancer.

Язык: Английский

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Associations between vitamins intake and risk of cancer in United States adults: 2003 to 2016 national health and nutrition examination survey

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Апрель 2, 2025

Introduction National Health and Nutrition Examination Survey (NHANES) is a cross-sectional survey that gathered information about people’s health nutrition. The aim of this study to investigate potential associations between vitamin intake cancer risk using database. Methods NHANES data set encompassed wide range variables, including vitamins, cancer, others. Logistic regression models, restricted cubic splines (RCS) subgroup analysis were used test the link consumption risk. Results In total, 29, 138 individuals included in study, while 2,924 them had diagnosis cancer. odds developing reduced for persons consuming highest quartile dietary niacin compared those lowest [odds ratio (OR) = 0.78, 95% confidence 0.65, 0.95, p 0.015]. However, after adjusting all confounding factors, as A gradually increased, tumor occurrence correspondingly increased (OR 1.38, CI 1.13, 1.69, 0.002). Subgroup RCS models showed similar results. Only when folic acid 267–367 mcg, positively correlated with tumors. Vitamins E, B1, B2, B6, B12, C, K, alpha-carotene beta-carotene not associated development. Conclusion Vitamin tumors, negatively incidence Further longitudinal studies are needed verify these findings.

Язык: Английский

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Effects of four-week lasting aerobic treadmill training on hepatic injury biomarkers, oxidative stress parameters, metabolic enzymes activities and histological characteristics in liver tissue of hyperhomocysteinemic rats

Molecular and Cellular Biochemistry, Год журнала: 2024, Номер unknown

Опубликована: Окт. 9, 2024

Язык: Английский

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Mitochondrial topoisomerase I (Top1MT) prevents the onset of metabolic dysfunction-associated steatohepatitis (MASH) in mice

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 5, 2024

Abstract High fat (HF) diet is a major factor in the development of metabolic dysfunction-associated steatotic liver disease (MASLD) and steatohepatis (MASH), mitochondria have been proposed to play role pathogenesis HF diet-induced MASH. Because Mitochondrial topoisomerase I (Top1MT) exclusively present Top1MT knock-out mice are viable, we were able assess We show that after 16 weeks diet, lacking prone severe MASH characterized by steatosis, lobular inflammation hepatocyte damage. Mice also prominent mitochondrial dysfunction, ROS production DNA (mtDNA) release, accompanied hepatic fibrosis. In summary, our study demonstrates importance sustaining functions suppressing

Язык: Английский

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Multi-omic analysis identifies the molecular mechanism of hepatocellular carcinoma with cirrhosis

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Окт. 11, 2024

Hepatocellular carcinoma with cirrhosis promotes the advancement of malignancy and development fibrosis in normal liver tissues. Understanding pathological mechanisms underlying HCC is important for developing effective therapeutic strategies. Herein, RNA-sequencing (RNA-seq) data corresponding clinical features patients were extracted from The Cancer Genome Atlas (TCGA) database using University California Santa Cruz (UCSC) Xena platform. enrichment degree hallmarkers each TCGA-LIHC cohort was quantified by ssGSEA algorithm. Weighted gene co-expression network analysis (WGCNA) revealed two module eigengenes (MEs) associated cirrhosis, namely, MEbrown MEgreen. Analysis these modules AUCell showed that had higher scores all immune cells, whereas MEgreen malignant cells. CellChat package both cells contributed to fibrotic activity myofibroblasts through diverse signaling pathways. Additionally, spatial transcriptomic hepatocytes, proliferating macrophages, located closer proximity These may potentially participate process stimulating myofibroblast activity, which be related fibrosis. In summary, we made full use multi-omics explore networks cell types involved progression HCC.

Язык: Английский

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