Anti-Inflammatory and Anti-Fibrotic Effects of a Mixture of Polyphenols Extracted from “Navelina” Orange in Human Hepa-RG and LX-2 Cells Mediated by Cannabinoid Receptor 2

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 512 - 512

Опубликована: Янв. 9, 2025

Navelina oranges (Citrus sinensis) are rich in phytonutrients and bioactive compounds, especially flavonoids like hesperidin. This study investigates the anti-inflammatory anti-fibrotic properties of hesperidin (HE) a polyphenol mixture from (OE) human hepatocytes (Hepa-RG) hepatic stellate cells (LX-2), order to elucidate underlying molecular mechanisms. In Hepa-RG cells, HE treatment increased expression cannabinoid receptor 2 (CB2R), which was associated with down-regulation p38 mitogen-activated protein kinases (p38 MAPK) but had minimal impact on cyclooxygenase-2 (COX-2) transforming growth factor-β (TGF-β) levels. Conversely, OE not only enhanced CB2R levels reduced MAPK, also promoted significant reduction both COX-2 TGF-β levels, suggesting that might be more effective mitigating inflammatory fibrotic processes than HE. LX-2 caused notable decrease reflecting its efficacy targeting fibrosis-associated inflammation. treatment, other hand, Nuclear Factor-Kappa B p65 (NF-κB) expression, critical transcription factor involved responses, though it did significantly affect COX-2. induced fibrosis treated showed several markers. addition, antioxidant effects by increasing Cu, Zn superoxide dismutase (SOD1), Mn (SOD2) catalase (CAT) influencing state lipid peroxidation. Further research is needed explore treatments activated vivo liver disease models.

Язык: Английский

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Adherence to the planetary health diet index and metabolic dysfunction-associated steatotic liver disease: a cross-sectional study

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Фев. 20, 2025

Adherence to the Planetary Health Diet Index (PHDI) has been shown benefit both individual health and planet. However, its impact on Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) remains unclear. This study aimed investigate relationship between PHDI adherence MASLD risk. We analyzed a cohort of 15,865 adults (aged ≥18 years) using data from National Nutrition Examination Survey (NHANES, 2005-2018). The was derived 24-h dietary assessments comprised scores 15 food groups. Multivariate logistic regression used association MASLD, while restricted cubic spline (RCS) threshold analysis were employed explore potential non-linear relationship. Subgroup analyses conducted assess influence various demographic clinical characteristics observed associations. Mediation performed evaluate indirect effect weighted quantile sum (WQS) nutrients MASLD. Among cohort, 6,125 individuals diagnosed with revealed that higher quintile significantly associated reduced risk in fully adjusted model (OR = 0.610, 95%CI 0.508-0.733, p < 0.001). Notably, nonlinear relationships through RCS (p 0.002). indicated particularly effective reducing among females, those education attainment, living partner. WQS identified saturated fatty acids as most significant factor contributing (weight 0.313). Additionally, BMI waist circumference (81.47 87.66%, respectively) partially mediated risk, suggesting operates, part, circumference. remained robust across multiple sensitivity analyses. Our findings indicate is linked lower providing crucial insights for strategies at mitigating epidemic simultaneously fostering environmental sustainability.

Язык: Английский

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Clinical Insights into Non-Alcoholic Fatty Liver Disease and the Therapeutic Potential of Flavonoids: An Update

Nutrients, Год журнала: 2025, Номер 17(6), С. 956 - 956

Опубликована: Март 9, 2025

Non-alcoholic fatty liver disease (NAFLD) is considered a significant global health issue related to serious metabolic disorders. However, effective pharmacological treatments are still limited. Flavonoids, wide group of polyphenol substances, exert anti-inflammatory and lipid-lowering effects in preclinical data. Thus, implementing these research findings clinical practice could significantly help manage NAFLD its consequences. This narrative review assesses the therapeutic potential flavonoids managing NAFLD. The collected randomized controlled trials (RCTs) meta-analyses RCTs from past five years concerning impact on A total 20 studies were selected according predetermined inclusion criteria, comprising thirteen seven meta-analyses. underscores beneficial management through enhancement lipid metabolism, reduction hepatic steatosis, provision actions. Clinical demonstrate that interventions rich flavonoids, including quercetin, epigallocatechin gallate, naringenin, isoflavones, substantially reduce fat content enhance enzyme profiles, with certain compounds exhibiting superior efficacy particular subgroups, such as older adults females. Nonetheless, whereas therapies diminish their effect fibrosis constrained. To sum up, exhibit supplementary for by enhancing function, inflammation. Additional extensive necessary create uniform treatment methods ascertain long-term advantages.

Язык: Английский

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Mitochondria at the Crossroads: Linking the Mediterranean Diet to Metabolic Health and Non-Pharmacological Approaches to NAFLD

Nutrients, Год журнала: 2025, Номер 17(7), С. 1214 - 1214

Опубликована: Март 30, 2025

Nonalcoholic fatty liver disease (NAFLD) is a growing global health concern that closely linked to metabolic syndrome, yet no approved pharmacological treatment exists. The Mediterranean diet (MD) emerged as first-line dietary intervention for NAFLD, offering and hepatoprotective benefits. Now conceptualized complex chemical matrix rich in bioactive compounds, the MD exerts antioxidant anti-inflammatory effects, improving insulin sensitivity lipid metabolism. Mitochondria play central role NAFLD pathophysiology, influencing energy metabolism, oxidative stress, homeostasis. Emerging evidence suggests MD's compounds enhance mitochondrial function by modulating phosphorylation, biogenesis, mitophagy. However, most research has focused on individual rather than whole, leaving gaps understanding its collective impact pattern. This narrative review explores how influence highlighting key pathways such substrate control, dynamics, efficiency. A literature search was conducted identify relevant studies MD, mitochondria, NAFLD. While promising, our remains incomplete, particularly when current knowledge limited lack of mechanistic comprehensive holistic impact. Future integrating cutting-edge experimental approaches needed elucidate intricate diet-mitochondria interactions. deeper influences essential developing precision-targeted nutritional strategies can effectively prevent manage disease.

Язык: Английский

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Changes in Liver Metabolome Induced by Pterostilbene and Resveratrol in a Rat Model of Liver Steatosis

Molecular Nutrition & Food Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 30, 2025

ABSTRACT To gain more light on the effects of resveratrol and pterostilbene in hepatic metabolic modifications an vivo model diet‐induced steatosis, to explore their relationships with gut microbiota by untargeted metabolomics metagenomics. Rats were divided into five groups receiving either a standard diet or high‐fat high‐fructose (HFHF) supplemented not (15 30 mg/kg body weight/day; PT15 PT30 groups, respectively) (30 RSV30 group). Supplementation stilbenes reduced steatosis induced HFHF diet. After study, 27 differentially expressed metabolites showed variable importance projection scores > 1 could be considered as potential biomarkers. Therefore, based pathway enrichment analysis, “riboflavin metabolism” “nicotinate nicotinamide revealed significant enrichment. Further, riboflavin positive correlations Eubacterium Faecalibacterium , negative Lactobacillus Oscillospira genera. Nicotinamide mononucleotide was only positively correlated Ralstonia genus. The approach that actions prevention liver are mediated specific mechanisms action. Particularly, pterostilbene, but resveratrol, is suggested significantly enrich nicotinate pathways.

Язык: Английский

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Flavonoids and Their Role in Preventing the Development and Progression of MAFLD by Modifying the Microbiota

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11187 - 11187

Опубликована: Окт. 17, 2024

With the increasing prevalence and serious health consequences of metabolic-associated fatty liver disease (MAFLD), early diagnosis intervention are key to effective treatment. Recent studies highlight important role dietary factors, including use flavonoids, in improving health. These compounds possess anti-inflammatory, antioxidant, liver-protective properties. Flavonoids have been shown affect gut microbiota, which plays a function progression. Therefore, their preventing development progression MAFLD through modulation microbiome seems be interest. This narrative review aims consolidate current evidence on effects selected flavonoids progression, potential mechanisms action, implications for personalized interventions management disease.

Язык: Английский

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Metataxonomics and Metabolomics Profiles in Metabolic Dysfunction-Associated Fatty Liver Disease Patients on a “Navelina” Orange-Enriched Diet

Nutrients, Год журнала: 2024, Номер 16(20), С. 3543 - 3543

Опубликована: Окт. 18, 2024

Metabolic dysfunction-associated fatty liver disease (MAFLD) is currently the most common cause of chronic disease. Systemic inflammatory status and peripheral metabolic symptoms in clinical picture have an impact on gut commensal bacteria.

Язык: Английский

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