In Vitro Inhibition of Colon Cancer Stem Cells by Natural Polysaccharides Obtained from Wheat Cell Culture DOI Open Access
A. S. Murtazina, Yaiza Jiménez-Martínez,

Gloria Ruiz Alcalá

и другие.

Polymers, Год журнала: 2025, Номер 17(8), С. 1048 - 1048

Опубликована: Апрель 12, 2025

Natural polysaccharides (PSs) have shown inhibitory effects on differentiated cancer cells (DCCs), but their activity against stem (CSCs) remains poorly understood. Here, we report that PSs from wheat cell cultures (WCCPSs) inhibit the proliferation of both DCCs and CSCs derived HCT-116 colorectal cells. Among them, NA DC fractions showed strongest anti-CSC activity. NA, rich in xylose, was effective at lower concentrations, while DC, enriched xylose galacturonic acid (GalUA), exhibited higher potency, with a IC50 preferential doses. WCCPSs reduced β-catenin levels, some also downregulated Ep-CAM, CD44, c-Myc. Notably, increased caspase-3 without inducing cytochrome C caspase-8 overexpression, suggesting mechanism promoting CSC differentiation rather than apoptosis. Correlation analysis linked content to c-Myc expression, GalUA levels caspase-3. These results suggest WCCPS bioactivity may be related monosaccharide composition. Overall, our findings support potential wheat-derived as CSC-targeting agents suppress self-renewal promote differentiation, offering promising approach reduce tumor aggressiveness recurrence.

Язык: Английский

Therapeutic potential of flavonoids in gastrointestinal cancer: Focus on signaling pathways and improvement strategies (Review) DOI Creative Commons

Ye Ding,

Yong Yu

Molecular Medicine Reports, Год журнала: 2025, Номер 31(4), С. 1 - 34

Опубликована: Фев. 24, 2025

Flavonoids are a group of polyphenolic compounds distributed in vegetables, fruits and other plants, which have considerable antioxidant, anti‑tumor anti‑inflammatory activities. Several types gastrointestinal (GI) cancer the most common malignant tumors world. A large number studies shown that flavonoids inhibitory effects on cancer, they recognized as class potential drugs. Therefore, present review investigated molecular mechanisms treatment different GI summarized drug delivery systems commonly used to improve their bioavailability. First, classification therapeutic various human diseases were briefly introduced. Then, clarify mechanism action body, metabolic process body associated signaling pathways causing five discussed, well corresponding targets flavonoids. Finally, clinical settings, poor water solubility, low permeability inferior stability, lead absorption efficiency vivo. three widely summarized. Suggestions for improving bioavailability focus next stage research also put forward.

Язык: Английский

Процитировано

1

Investigating small molecules in Propolis as Nipah virus glycoprotein (NiV-G) inhibitors through molecular interaction studies DOI Creative Commons
Muaz Faruque, Md Afjalus Siraj, Md. Nazmul Hasan Zilani

и другие.

Heliyon, Год журнала: 2025, Номер 11(4), С. e42595 - e42595

Опубликована: Фев. 1, 2025

Despite the significant fatality rates associated with Nipah virus (NiV) outbreaks in South Asia, including Bangladesh, and India, till today, there is no approved medications to treat it. In this context, small molecules propolis were computationally screened through pharmacokinetic toxicity studies followed by molecular docking dynamics simulation glycoprotein (NiV-G protein) assess their anti-Nipah potential. A thorough literature analysis was performed identify antiviral compounds from a pool of 84 experimental articles. Following ADMET analysis, 27 out 34 docked against NiV-G compared control ligand, ribavirin, which an investigational drug Nipah. The revealed that bauer-7-en-3β-yl acetate (BA) moronic acid (MA) bound more strongly active site than ribavirin other ligands. Investigation root-mean-square deviation (RMSD), root mean square fluctuations (RMSF), radius gyration (Rg), solvent accessible surface area (SASA), (MolSA), binding free energy (MM-PBSA), complexity hydrogen bonds (HBs), secondary structure ligand-target interactions for 100 ns (MD) study further supported complex's stability compactness. Therefore, silico interaction reports both may be possible candidates infection.

Язык: Английский

Процитировано

0

Enhancing Curcumol Delivery through PD-1 Targeted Nanocarriers: A Novel Therapeutic Approach for Prostate Cancer DOI
Hao Zhang, Yang Du, Xujia Liu

и другие.

Phytomedicine, Год журнала: 2025, Номер 141, С. 156595 - 156595

Опубликована: Март 2, 2025

Язык: Английский

Процитировано

0

Preliminary Insights into the Cyto/Genoprotective Properties of Propolis and Its Constituent Galangin In Vitro DOI Creative Commons
Mateo Jakac, Irena Brčić Karačonji, Andreja Jurić

и другие.

Toxics, Год журнала: 2025, Номер 13(3), С. 194 - 194

Опубликована: Март 8, 2025

Propolis has been well known for centuries as a natural preventive and therapeutic agent. Its numerous health benefits are mainly attributed to its high content of phenolic compounds that have remarkable antioxidant activity. Since phenolics may exert dual nature (pro-oxidant antioxidant) the aim this study was investigate safety profile ethanolic extract propolis related flavonoid galangin their ability protect lymphocytes from irinotecan-induced cyto/genotoxicity in vitro. Isolated human peripheral blood were exposed 3 h three concentrations corresponding average daily dose 0.25 mL [propolis 70% ethanol (3:7, w/w)], five- ten-fold higher concentration. Cyto- genoprotective effects tested using cytokinesis-block micronucleus cytome assay. Treatment with selected exerted biocompatibility diminished level cytogenetic damage caused by irinotecan. at same concentration offered stronger protective effect than single galangin. Also, apoptosis prevailing mechanism cell death our experimental conditions. These preliminary results speak favour future investigations other available methods models.

Язык: Английский

Процитировано

0

Network Pharmacology and Experimental Validation Reveal Therapeutic Potential of Propolis in UV-Induced Allergic Dermatitis DOI Creative Commons

Liyuan Cheng,

Jie Wang, Y. Wang

и другие.

Foods, Год журнала: 2025, Номер 14(6), С. 996 - 996

Опубликована: Март 14, 2025

Propolis demonstrates diverse pharmacological properties encompassing antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, and wound-healing activities. This study investigated the therapeutic mechanism of propolis against ultraviolet (UV)-induced allergic dermatitis through an integrated approach combining network pharmacology with in vitro experimental validation. The targets components were conducted PubChem, EMBL-EBI, SEA Search Server databases, disease-associated for atopic related conditions extracted from GeneCards. overlapping between UV-induced screened. Gene Ontology (GO) Enrichment analysis Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment performed. key further validated ELISA experiments using HSF cells. results show that there 28 dermatitis. GO 1246 terms biological functions, 52 cellular components, 98 molecular functions. KEGG obtained 110 signaling pathways. protein-protein interaction (PPI) showed TNF, NFKB1, MMP-9, IL-2 hub proteins. experiment confirmed reduced levels MMP-9 UBV-induced cells a dose-dependent manner. These findings provide mechanistic evidence supporting as promising functional food, dietary supplements, or medicinal agent skin disorders.

Язык: Английский

Процитировано

0

Targeting the MAPK signaling pathway: implications and prospects of flavonoids in 3P medicine as modulators of cancer cell plasticity and therapeutic resistance in breast cancer patients DOI Creative Commons
Peter Kubatka, Bianka Bojková, Natália Nosálová

и другие.

The EPMA Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Abstract Cancer drug resistance poses a significant challenge in oncology, primarily driven by cancer cell plasticity, which promotes tumor initiation, progression, metastasis, and therapeutic evasion many different cancers. Breast cancers (BCs) are prominent example of that, with an estimated 2.3 million new cases 670,000 BC-related deaths registered worldwide annually. Triple-negative BC is especially challenging for treatments demonstrating particularly aggressive disease course, early manifestation metastatic disease, frequent drug-resistant types, poor individual outcomes. Although chemosensitizing agents have been developed, their clinical utility oncology remains unproven. The mitogen-activated protein kinase (MAPK) pathway considered critical regulator intracellular extracellular signaling highly relevant both — genetic epigenetic modifications. Dysregulation the MAPK pathways plays role conferring chemoresistance BC. Contextually, targeting represents promising strategy overcoming enhancing efficacy anticancer treatment. On other hand, flavonoids, class phytochemicals, key modulators signaling. Flavonoids interact ERK, JNK, p38, ERK5 cascade present avenue developing novel anti-cancer therapies re-sensitizing treatment Compounds such as quercetin, kaempferol, genistein, luteolin, myricetin, EGCG, baicalein, baicalin, nobiletin, morin, delphinidin, acacetin, isorhamnetin, apigenin, silymarin, among others, identified specific signaling, exerting complex downstream effects cells increasing therewith suppressing growth aggressivity. These properties reflect mechanisms great relevance to overcome overall management. This article highlights corresponding provides clinically illustrations framework 3P medicine primary (protection individuals at high risk against health-to-disease transition) secondary care progression). 3PM novelty makes good use patient phenotyping stratification, predictive multi-level diagnostics, application Artificial Intelligence (AI) tools individualized interpretation big data all proposed cost-effective tailored profiles clear benefits patients advanced

Язык: Английский

Процитировано

0

In Vitro Inhibition of Colon Cancer Stem Cells by Natural Polysaccharides Obtained from Wheat Cell Culture DOI Open Access
A. S. Murtazina, Yaiza Jiménez-Martínez,

Gloria Ruiz Alcalá

и другие.

Polymers, Год журнала: 2025, Номер 17(8), С. 1048 - 1048

Опубликована: Апрель 12, 2025

Natural polysaccharides (PSs) have shown inhibitory effects on differentiated cancer cells (DCCs), but their activity against stem (CSCs) remains poorly understood. Here, we report that PSs from wheat cell cultures (WCCPSs) inhibit the proliferation of both DCCs and CSCs derived HCT-116 colorectal cells. Among them, NA DC fractions showed strongest anti-CSC activity. NA, rich in xylose, was effective at lower concentrations, while DC, enriched xylose galacturonic acid (GalUA), exhibited higher potency, with a IC50 preferential doses. WCCPSs reduced β-catenin levels, some also downregulated Ep-CAM, CD44, c-Myc. Notably, increased caspase-3 without inducing cytochrome C caspase-8 overexpression, suggesting mechanism promoting CSC differentiation rather than apoptosis. Correlation analysis linked content to c-Myc expression, GalUA levels caspase-3. These results suggest WCCPS bioactivity may be related monosaccharide composition. Overall, our findings support potential wheat-derived as CSC-targeting agents suppress self-renewal promote differentiation, offering promising approach reduce tumor aggressiveness recurrence.

Язык: Английский

Процитировано

0