Vascularized characteristics and functional regeneration of three-dimensional cell reconstruction of oral mucosa equivalents based on vascular homeostasis phenotypic modification DOI Creative Commons

Lijuan Shi,

Yiwen Xu, Jingying Li

и другие.

Journal of Tissue Engineering, Год журнала: 2024, Номер 15

Опубликована: Янв. 1, 2024

Our prior research has effectively developed tissue-engineered vascularized oral mucosa equivalents (VOME); however, challenges such as low repeatability and stability, well the inability to accurately replicate complexity of real blood vessels, were encountered. Therefore, this study aimed screen VOME native vascular homeostasis phenotypes by tandem mass tag-tagged proteomics associated with laser capture microdissection human angiogenesis antibody array technology. Then, lentiviruses constructed stably transfected endothelial-like cells (VELCs) detect angiogenic capacity. HE, EdU Apollo tracer staining, immunofluorescence scanning electron microscopy, biomechanical testing, a small animal ultrasound imaging system used analyze characteristics vascularization monitor functional regeneration homeostatic phenotypic mucosal (VHPOME). The results showed that PGAM1, COL5A1, ANG, RNH1 are potential specific phenotypes. High expression ANG and/or can promote VOME. ANG/shRNH1 most significant tube-like structure-formation ability. in VHPOME group was higher than control group, lower group. COL5A1/ANG significantly improve mechanical properties. flow signal shRNH1, PGAM1/ANG, COL5A1/ANG, PGAM1/shRNH1, COL5A1/shRNH1, may be targets for establishing equivalent genes (groups), effect.

Язык: Английский

Saponins enhance the stability and cost-efficiency of human embryonic stem cell culture DOI Creative Commons
Jingyi Shi, Mei Wu, Fang Shi

и другие.

Cell Regeneration, Год журнала: 2025, Номер 14(1)

Опубликована: Янв. 21, 2025

Abstract The cultivation and differentiation of human embryonic stem cells (hESCs) into organoids are crucial for advancing new drug development personalized cell therapies. Despite establishing chemically defined hESC culture media over the past decade, these media's reliance on growth factors, which costly prone to degradation, poses a challenge sustained stable culture. Here, we introduce an system(E6Bs) that facilitates long-term, genetically expansion hESCs, enabling consistently sustain high levels pluripotency markers, including NANOG, SOX2, TRA-1–60, SSEA4, across extended periods. Moreover, derived from hESCs using this medium were successfully established expanded at least one month, exhibiting cortical organoids, GABAergic precursor heart-forming organoids. This innovative system offers robust tool preserving homeostasis modeling nervous in vitro.

Язык: Английский

Процитировано

0
Three-Dimensional-Bioprinted Embedded-Based Cerebral Organoids: An Alternative Approach for Mini-Brain In Vitro Modeling Beyond Conventional Generation Methods DOI Creative Commons
Rosalba Monica Ferraro, Paola Serena Ginestra, Miriam Seiti

и другие.

Gels, Год журнала: 2025, Номер 11(4), С. 284 - 284

Опубликована: Апрель 11, 2025

Cerebral organoids (cORGs) obtained from induced pluripotent stem cells (iPSCs) have become significant instruments for investigating human neurophysiology, with the possibility of simulating diseases and enhancing drug discovery. The current approaches require a strict process manual inclusion in animal-derived matrix Matrigel® are challenged by unpredictability, operators’ skill expertise, elevated costs, restricted scalability, impeding their extensive applicability translational potential. In this study, we present novel method to generate brain that address these limitations. Our approach does not manual, operator-dependent embedding. Instead, it employs chemically defined hydrogel which is diluted solution enriched sodium alginate (SA) carboxymethylcellulose (CMC) used as bioink print neural embryoid bodies (nEBs). Immunohistochemical, immunofluorescence, gene expression analyses confirmed SA-CMC-Matrigel® can sustain generation iPSC-derived cortical cORGs conventional Matrigel®-based does. By day 40 differentiation, hydrogel-based 3D-bioprinted showed heterogeneous consistent masses, cytoarchitecture resembling an early-stage developmental fetal composed progenitor PAX6+/Ki67+ organized into tubular structures, densely packed cell somas neurites SYP+, suggestive tissue-like neuronal layer formation.

Язык: Английский

Процитировано

0
Advancements in Human Norovirus Cultivation in Human Intestinal Enteroids DOI Open Access
Khalil Ettayebi, Gurpreet Kaur, Ketki Patil

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 24, 2024

Human noroviruses (HuNoVs) are a significant cause of epidemic and sporadic acute gastroenteritis worldwide. The lack reproducible culture system hindered the study HuNoV replication pathogenesis for almost half-century. This barrier was overcome with our successful cultivation multiple strains in human intestinal enteroids (HIEs), which has significantly advanced research. We optimized media conditions generated genetically-modified HIE cultures to enhance HIEs. Building upon these achievements, we now present new insights this system, involve testing different media, unique lines, additional virus strains. infectivity evaluated compared models, including HIEs from segments individual adult organ donors, organoids produced directed differentiation embryonic stem cells into that were transplanted matured mice before making (H9tHIEs), genetically-engineered (J4

Язык: Английский

Процитировано

2
Loss ofUBE3Aimpacts both neuronal and non-neuronal cells in human cerebral organoids DOI Creative Commons
R. Chris Estridge, Z. Begum Yagci,

Dilara Sen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 10, 2024

Abstract Angelman syndrome is a neurodevelopmental disorder caused by (epi)genetic lesions of maternal UBE3A . Research has focused largely on the role in neurons due to its imprinting that cell type. Yet, evidence suggests there may be broader impacts dysregulation. Human cerebral organoids might reveal these understudied aspects as they recapitulate diverse types developing human brain. We performed scRNAseq effects disruption type-specific compositions and transcriptomic alterations. In absence , progenitor proliferation structures were disrupted while organoid composition shifted away from proliferative types. observed non-neuronal cells, including choroid plexus enrichment. Furthermore, EMX1+ cortical progenitors negatively impacted, disrupting corticogenesis, potentially delaying excitatory neuron maturation. This work reveals novel related processes elucidates potential new therapeutic targets. Teaser exhibit compositional alterations both neuronal cells

Язык: Английский

Процитировано

1
Vascularized characteristics and functional regeneration of three-dimensional cell reconstruction of oral mucosa equivalents based on vascular homeostasis phenotypic modification DOI Creative Commons

Lijuan Shi,

Yiwen Xu, Jingying Li

и другие.

Journal of Tissue Engineering, Год журнала: 2024, Номер 15

Опубликована: Янв. 1, 2024

Our prior research has effectively developed tissue-engineered vascularized oral mucosa equivalents (VOME); however, challenges such as low repeatability and stability, well the inability to accurately replicate complexity of real blood vessels, were encountered. Therefore, this study aimed screen VOME native vascular homeostasis phenotypes by tandem mass tag-tagged proteomics associated with laser capture microdissection human angiogenesis antibody array technology. Then, lentiviruses constructed stably transfected endothelial-like cells (VELCs) detect angiogenic capacity. HE, EdU Apollo tracer staining, immunofluorescence scanning electron microscopy, biomechanical testing, a small animal ultrasound imaging system used analyze characteristics vascularization monitor functional regeneration homeostatic phenotypic mucosal (VHPOME). The results showed that PGAM1, COL5A1, ANG, RNH1 are potential specific phenotypes. High expression ANG and/or can promote VOME. ANG/shRNH1 most significant tube-like structure-formation ability. in VHPOME group was higher than control group, lower group. COL5A1/ANG significantly improve mechanical properties. flow signal shRNH1, PGAM1/ANG, COL5A1/ANG, PGAM1/shRNH1, COL5A1/shRNH1, may be targets for establishing equivalent genes (groups), effect.

Язык: Английский

Процитировано

0