Longitudinal SARS-CoV-2 RNA wastewater monitoring across a range of scales correlates with total and regional COVID-19 burden in a well-defined urban population

Water Research, Год журнала: 2022, Номер 220, С. 118611 - 118611

Опубликована: Май 14, 2022

Wastewater-based epidemiology (WBE) is an emerging surveillance tool that has been used to monitor the ongoing COVID-19 pandemic by tracking SARS-CoV-2 RNA shed into wastewater. WBE was performed occurrence and spread of from three wastewater treatment plants (WWTP) six neighborhoods in city Calgary, Canada (population 1.44 million). A total 222 WWTP 192 neighborhood samples were collected June 2020 May 2021, encompassing end first-wave (June 2020), second-wave (November December 2020) third-wave (mid-April 2021). Flow-weighted 24-hour composite processed extract then analyzed for two SARS-CoV-2-specific regions nucleocapsid gene, N1 N2, using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Using this approach detected 98.06% (406/414) samples. abundance compared clinically diagnosed cases organized three-digit postal code affected individuals' primary residences, enabling correlation analysis at neighborhood, city-wide scales. Strong correlations observed between & N2 gene signals new daily WWTPs neighborhoods. Similarly, when flow rates Calgary's normalize concentrations combine them a signal, strongly correlated with regionally clinical test percent positivity rate. Linked census data demonstrated disproportionate areas lower socioeconomic status more racialized communities. across range urban scales be effective mechanism surveillance.

Язык: Английский

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Dengue as a Disease Threatening Global Health: A Narrative Review Focusing on Latin America and Brazil

Tropical Medicine and Infectious Disease, Год журнала: 2023, Номер 8(5), С. 241 - 241

Опубликована: Апрель 23, 2023

Arboviruses constitute the largest known group of viruses. These viruses are etiological agents pathologies as arboviruses, with dengue being one most prevalent. Dengue has resulted in important socioeconomic burdens placed on different countries around world, including those Latin America, especially Brazil. Thus, this work intends to carry out a narrative-based review literature, conducted using study secondary data developed through survey scientific literature databases, and present situation dengue, particularly its distribution these localities. Our findings from demonstrate difficulties that managers face controlling spread planning response against pointing high cost disease for public coffers, rendering resources already limited even scarcer. This can be associated factors affect disease, ecological, environmental, social factors. order combat it is expected targeted properly coordinated policies need adopted not only specific localities, but also globally.

Язык: Английский

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An interpretative review of the wastewater-based surveillance of the SARS-CoV-2: where do we stand on its presence and concern?

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Янв. 22, 2024

Wastewater-based epidemiology (WBE) has been used for monitoring infectious diseases like polio, hepatitis, etc. since the 1940s. It is also being tracking SARS-CoV-2 at population level. This article aims to compile and assess information qualitative quantitative detection of in wastewater. Based on globally published studies, we highlight importance presence/detection wastewater concurrently emphasize development early surveillance techniques. RNA sheds human feces, saliva, sputum mucus that ultimately reaches brings viral into it. For virus wastewater, different techniques have optimized are use. These based serological, biosensor, targeted PCR, next generation sequencing whole genome or amplicon sequencing. The presence could be as a potential tool devising strategies eradication before it spread community. Additionally, with right timely understanding behavior environment, an accurate instructive model leverages WBE-derived data may created. might help creation technological tools doable plans action lessen negative effects current epidemics future outbreaks public health economy. Further work toward whether load correlates its ability induce infection, still needs evidence. increasing incidences JN.1 variant case point continued surveillance, including

Язык: Английский

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Optimized Aluminum Hydroxide Adsorption–Precipitation for Improved Viral Detection in Wastewater

International Journal of Environmental Research and Public Health, Год журнала: 2025, Номер 22(2), С. 148 - 148

Опубликована: Янв. 23, 2025

Wastewater-based epidemiology (WBE) is a valuable tool for monitoring pathogen spread in communities; however, current protocols mainly target non-enveloped viruses. This study addresses the need standardized methods to detect both enveloped and viruses by testing four aluminum hydroxide adsorption–precipitation techniques. Wastewater samples were spiked with an virus surrogate (Φ6 bacteriophage) (MS2 coliphage), viral recovery was assessed using reverse-transcription quantitative PCR (RT-qPCR). The highest achieved AlCl3 at pH 3.5, 15 min flocculation time, 3% elution solution concentration. For virus, optimal found 6.0, no 10% solution. best method recovering types used flocculation, shows that while conditions vary between types, protocol can efficiently recover both, providing cost-effective approach outbreak Grenada.

Язык: Английский

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Wastewater-based epidemiology for preventing outbreaks and epidemics in Latin America – Lessons from the past and a look to the future

The Science of The Total Environment, Год журнала: 2022, Номер 865, С. 161210 - 161210

Опубликована: Дек. 27, 2022

Wastewater-based epidemiology (WBE) is an approach with the potential to complement clinical surveillance systems. Using WBE, it possible carry out early warning of a outbreak, monitor spatial and temporal trends infectious diseases, produce real-time results generate representative epidemiological information in territory, especially areas social vulnerability. Despite historical uses this approach, particularly Global Polio Eradication Initiative, for other pathogens, was during COVID-19 pandemic that occurred exponential increase environmental programs SARS-CoV-2 wastewater, many experiences developments field public health using data decision making prioritizing actions control pandemic. In Latin America, WBE applied heterogeneous contexts emphasis on populations present socio-environmental inequalities, condition shared by all American countries. This manuscript addresses concepts applications actions, as well different countries, discusses model implement system at local or national level. We emphasize need sentinel countries want detect entry spread new pathogens outbreaks epidemics agents their territories

Язык: Английский

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Detecting SARS-CoV-2 lineages and mutational load in municipal wastewater and a use-case in the metropolitan area of Thessaloniki, Greece

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Фев. 17, 2022

Abstract The COVID-19 pandemic represents an unprecedented global crisis necessitating novel approaches for, amongst others, early detection of emerging variants relating to the evolution and spread virus. Recently, SARS-CoV-2 RNA in wastewater has emerged as a useful tool monitor prevalence virus community. Here, we propose methodology, called lineagespot , for monitoring mutations lineages samples using next-generation sequencing (NGS). Our proposed method was tested evaluated NGS data produced by 14 from municipality Thessaloniki, Greece, covering 6-month period. results showed presence data. able record rapid domination Alpha variant (B.1.1.7) community, allowed correlation between evident through our approach observed patients same area time periods. is open-source tool, implemented R, freely available on GitHub registered bio.tools.

Язык: Английский

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Effects of Temperature and Water Types on the Decay of Coronavirus: A Review

Water, Год журнала: 2023, Номер 15(6), С. 1051 - 1051

Опубликована: Март 9, 2023

The analysis of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) gene copy numbers in wastewater samples can provide quantitative information on Disease-19 (COVID-19) cases within a sewer catchment. However, many wastewater-based epidemiology (WBE) studies have neglected virus decay during the transportation process sewers while back-calculating COVID-19 prevalence. Among various condition parameters, temperature and dilution by fresh/saltwater infiltration may result significant change to decay, terms both infectivity Ribonucleic Acid (RNA). This paper reviewed literature identify discuss effects water types (i.e., wastewater, freshwater, seawater) coronavirus based rate constants that were collected from published papers. To evaluate importance sensitivity was then conducted with rates SARS-CoV-2 RNA WBE back-calculation equation. Finally, also compared those other viruses further understand difference among species. found be less impacted variation than viable coronaviruses. Nevertheless, still sensitive increased warm over 26 °C), which could lead two-times higher relative variance estimated prevalence, considering between 4 37 °C catchment 12-h hydraulic retention time. Comparatively, estimation enveloped greater nonenveloped enteric viruses, easily degradable wastewater. In addition, stormwater inflow accompanied cold weather might alleviate infectivity, thus increasing potential risk transmission through Overall, this aims better impact in-sewer processes its implications for WBE. outcome quantitatively inform improve awareness infection via heavy rainfall events. Given identified scarcity data available salt or chemical additions, future research fate subjected dosing treatment plant operations is recommended.

Язык: Английский

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Predictive potential of SARS-CoV-2 RNA concentration in wastewater to assess the dynamics of COVID-19 clinical outcomes and infections

The Science of The Total Environment, Год журнала: 2023, Номер 886, С. 163935 - 163935

Опубликована: Май 8, 2023

Coronavirus disease 2019 - caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) -, has triggered a worldwide pandemic resulting in 665 million infections and over 6.5 deaths as of December 15, 2022. The development different epidemiological tools have helped predict new outbreaks assess behavior clinical variables health contexts. In this study, we aimed to monitor concentrations SARS-CoV-2 wastewater tool progression during Waves 3, 5, 6 Spanish city Xátiva from September 2020 March We estimated RNA 195 samples using RT-PCR Diagnostic Panel validated Center for Disease Control Prevention. also compared trends several (14-day cumulative incidence, positive cases, hospital cases stays, critical primary care visits, deaths) each study wave against Pearson's product-moment correlations, two-sided Mann-Whitney U test, cross-correlation analysis. found strong correlations between with 14-day incidence time. Wastewater showed these one two weeks advance. There were significant hospitalizations Wave 3 6; cross-correlations stronger hospitalization stays week before 6. No association vaccination percentages viral was observed. Our findings support potential surveillance anticipate infection data such hospitalizations, stays. Public authorities could use on similar population an aid decision-making epidemic outbreak.

Язык: Английский

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Comparison of the methods for isolation and detection of SARS-CoV-2 RNA in municipal wastewater

Frontiers in Public Health, Год журнала: 2023, Номер 11

Опубликована: Март 7, 2023

Coronavirus SARS-CoV-2 is a causative agent responsible for the current global pandemic situation known as COVID-19. Clinical manifestations of COVID-19 include wide range symptoms from mild (i.e., cough, fever, dyspnea) to severe pneumonia-like respiratory symptoms. has been demonstrated be detectable in stool patients. Waste-based epidemiology (WBE) shown promising approach early detection and monitoring local population performed via collection, isolation, viral pathogens environmental sources.In order select optimal protocol epidemiological region Turiec, Slovakia, we (1) compared methods separation including virus precipitation by polyethylene glycol (PEG), purification ultrafiltration (Vivaspin®) subsequent isolation NucleoSpin RNA Virus kit (Macherey-Nagel), direct wastewater (Zymo Environ Water Kit); (2) evaluated impact water freezing on SARS- CoV-2 separation, detection; (3) role filtration stability; (4) determined appropriate reverse transcription-droplet digital PCR (RT-ddPCR) real-time quantitative polymerase chain reaction (RT-qPCR) (targeting same genes, i.e., RdRp gene E) samples.(1) Usage Zymo Kit provided superior quality isolated comparison with both ultracentrifugation PEG precipitation. Freezing samples significantly reduces yield. Filtering counterproductive when used. According specificity sensitivity, RT-ddPCR outperforms RT-qPCR.The results our study suggest that WBE valuable warning alert represents non-invasive monitor pathogens, thus protects public health regional national level. In addition, have sensitivity testing nearer limit can improved selecting combination enrichment, methods.

Язык: Английский

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Outbreak of COVID-19: A Detailed Overview and Its Consequences

Advances in experimental medicine and biology, Год журнала: 2021, Номер unknown, С. 23 - 45

Опубликована: Янв. 1, 2021

Язык: Английский

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