PET
imaging
of
bacterial
infection
could
potentially
provide
added
benefit
for
patient
care
by
non-invasive
means.
[68Ga]Ga-desferrioxamine
B
–
a
radiolabelled
siderophore
shows
specific
uptake
human-pathogenic
bacteria
like
Staphylococcus
aureus
or
Pseudomonas
aeruginosa
as
well
sufficient
serum
stability
clinical
application.
Here,
we
data
automated
production
on
two
different
cassette
based
synthesis
modules
(Modular-Lab
PharmTracer
and
GRP
3V)
using
commercially
available
cassettes
together
with
an
approved
68Ge/68Ga
radionuclide
generator.
Quality
control
including
determination
radiochemical
purity
system
suitability
test
was
set
up
via
RP-HPLC
C18
column.
The
described
processes,
acetic
acid/acetate
buffer
ascorbic
acid
radiation
scavenger
radiolabelling,
yield
ready-to-use
formulations
activity
yield.
Batch
showed
>95
%
determined
ITLC
excellent
over
2
hours
post
production.
Specifications
routine
were
validated
four
master
batches
each
module.
Based
this
study,
academic
trial
initiated.
Both
methods
enable
reproducible,
in-house
Pathogens,
Год журнала:
2023,
Номер
12(4), С. 623 - 623
Опубликована: Апрель 20, 2023
Urinary
tract
infections
(UTIs)
are
among
the
most
common
bacterial
worldwide,
occurring
in
both
community
and
healthcare
settings.
Although
clinical
symptoms
of
UTIs
heterogeneous
range
from
uncomplicated
(uUTIs)
to
complicated
(cUTIs),
usually
treated
empirically.
Bacteria
main
causative
agents
these
infections,
although
more
rarely,
other
microorganisms,
such
as
fungi
some
viruses,
have
been
reported
be
responsible
for
UTIs.
Uropathogenic
Escherichia
coli
(UPEC)
is
agent
uUTIs
cUTIs,
followed
by
pathogenic
Klebsiella
pneumoniae,
Proteus
mirabilis,
Enterococcus
faecalis,
Staphylococcus
spp.
In
addition,
incidence
caused
multidrug
resistance
(MDR)
increasing,
resulting
a
significant
increase
spread
antibiotic
economic
burden
infections.
Here,
we
discuss
various
factors
associated
with
UTIs,
including
mechanisms
pathogenicity
related
bacteria
that
cause
emergence
increasing
UTI
pathogens.
Diagnostics,
Год журнала:
2023,
Номер
13(6), С. 1016 - 1016
Опубликована: Март 7, 2023
IgG
N-glycans
are
an
emerging
source
of
disease-specific
biomarkers.
Over
the
last
decade,
continued
development
glycomic
databases
and
evolution
glyco-analytic
methods
have
resulted
in
increased
throughput,
resolution,
sensitivity.
promote
adaptive
immune
responses
through
antibody-dependent
cellular
cytotoxicity
(ADCC)
complement
activation
to
combat
infection
or
cancer
autoimmunity.
In
addition
functional
assays,
researchers
examining
ability
protein-specific
glycosylation
serve
as
biomarkers
disease.
This
literature
review
demonstrates
that
can
discriminate
between
healthy
controls,
autoimmune
disease,
infectious
with
high
The
also
indicates
patterns
vary
across
disease
state,
thereby
supporting
their
role
specific
addition,
be
collected
longitudinally
from
patients
track
treatment
predict
reoccurrence.
focuses
on
N-glycan
profiles
applied
diagnostics,
cohort
discriminators,
prognostics.
Recent
successes,
remaining
challenges,
upcoming
approaches
critically
discussed.
Pharmaceutics,
Год журнала:
2023,
Номер
15(3), С. 926 - 926
Опубликована: Март 13, 2023
The
combination
of
two
or
more
agents
capable
acting
in
synergy
has
been
reported
as
a
valuable
tool
to
fight
against
pathogens.
Silver
nanoparticles
(AgNPs)
present
strong
antimicrobial
action,
although
their
cytotoxicity
for
healthy
cells
at
active
concentrations
is
major
concern.
Azoimidazole
moieties
exhibit
interesting
bioactivities,
including
activity.
In
this
work,
class
recently
described
azoimidazoles
with
antifungal
activity
was
conjugated
citrate
polyvinylpyrrolidone-stabilized
AgNPs.
Proton
nuclear
magnetic
resonance
used
confirm
the
purity
compounds
before
further
tests
and
atomic
absorption
spectroscopy
verify
concentration
silver
prepared
dispersions.
Other
analytical
techniques
elucidate
morphology
stability
AgNPs
corresponding
conjugates,
namely
ultraviolet-visible
spectrophotometry,
scanning
transmission
electron
microscopy
dynamic
light
scattering
analysis.
synergistic
conjugates
assessed
through
checkerboard
assay
yeasts
(Candida
albicans
Candida
krusei)
bacteria
(Staphylococcus
aureus
Escherichia
coli).
showed
improved
all
microorganisms,
particular
towards
bacteria,
below
individual
minimal
inhibitory
(MIC).
Furthermore,
some
combinations
were
found
be
non-cytotoxic
human
HaCaT
cells.
Pharmacological Reviews,
Год журнала:
2025,
Номер
unknown, С. 100038 - 100038
Опубликована: Янв. 1, 2025
Polypharmacological
approaches
have
significant
potential
for
the
treatment
of
various
complex
diseases,
including
infectious
bacteria-related
diseases.
Actually,
multitargeting
agents
can
achieve
better
therapeutic
effects
and
overcome
drawbacks
monotherapy.
Although
multidrug
multitarget
strategies
demonstrated
ability
to
inactivate
bacteria,
several
challenges
been
pointed
out.
In
this
way,
direct
ligands
appear
be
a
rational
sustainable
strategy
combat
antibiotic
resistance.
By
combining
different
pharmacophores,
hybrids
stand
out
as
promising
application
in
field
bacterial
infections.
These
new
chemical
entities
synergistic
interactions
that
allow
extend
spectrum
action
target
multiple
pathways.
addition,
reduce
likelihood
resistance
development
provide
improved
stability.
It
is
worth
highlighting
despite
efforts
scientific
community
discover
solutions
most
there
lack
studies
on
biofilm-associated
This
review
describes
polypharmacological
used
treat
infections
with
particular
focus,
whenever
possible,
those
promoted
by
biofilms.
exploring
these
innovative
approaches,
we
aim
inspire
further
research
progress
search
effective
treatments
biofilm-related
ones.
SIGNIFICANCE
STATEMENT:
The
importance
proposed
topic
lies
escalating
challenge
resistance,
particularly
context
such
hybrids,
represent
targeting
signaling
pathways,
not
only
enhance
effect
but
also
while
improving
drug's
Despite
urgent
need
notable
gap,
highlights
critical
motivating
advances
biofilm
related
Turkish Journal of Agriculture - Food Science and Technology,
Год журнала:
2025,
Номер
13(1), С. 237 - 243
Опубликована: Янв. 18, 2025
Molecular
approaches
used
to
identify
bacterial
species
use
16S
rRNA
and
MLST
determine
the
genetic
linkage
of
bacteria;
characterizes
clonal
linkages
by
examining
differences
in
various
gene
loci.
MLVA
determines
relationships
strains
biovar-level
assesses
copy
number
repeated
DNA
sequences.
Sequencing
provides
data
identifying
sequences;
Sanger
sequencing
is
basis
for
next-generation
approaches.
CRISPR
modifies
code
can
correct
mutations
or
control
genes
using
Cas9.
These
methods
are
important
annotating
genomic
information.
The
this
purpose
brought
together
study.
explanation
detailed
description
examined
will
contribute
their
field
microbiology.
Biology,
Год журнала:
2025,
Номер
14(2), С. 182 - 182
Опубликована: Фев. 11, 2025
Extracellular
vesicles
(EVs)
are
nanosized
that
secreted
by
all
cells
into
the
extracellular
space.
EVs
involved
in
cell-to-cell
communication
and
can
be
found
different
bodily
fluids
(bronchoalveolar
lavage
fluid,
sputum,
urine),
tissues,
circulation;
composition
of
reflects
physiological
condition
releasing
cell.
The
ability
to
use
from
for
minimally
invasive
detection
monitor
diseases
makes
them
an
attractive
target.
carry
a
snapshot
cell's
internal
state,
they
serve
as
powerful
biomarkers
diagnosing
diseases.
also
play
role
body's
immune
pathogen
responses.
Pathogens,
such
bacteria
viruses,
exploit
enhance
their
survival
spread
evade
system.
Changes
number
or
contents
signal
presence
infection,
offering
potential
avenue
developing
new
diagnostic
methods
infectious
Ongoing
research
this
area
aims
address
current
challenges
range
diseases,
including
infections
There
is
limited
literature
on
development
using
existing
molecular
biology
approaches.
We
aim
gap
reviewing
recent
EV-related
investigations
disease
studies.
