Genomic Analysis Methods of Microorganisms

Turkish Journal of Agriculture - Food Science and Technology, Год журнала: 2025, Номер 13(1), С. 237 - 243

Опубликована: Янв. 18, 2025

Molecular approaches used to identify bacterial species use 16S rRNA and MLST determine the genetic linkage of bacteria; characterizes clonal linkages by examining differences in various gene loci. MLVA determines relationships strains biovar-level assesses copy number repeated DNA sequences. Sequencing provides data identifying sequences; Sanger sequencing is basis for next-generation approaches. CRISPR modifies code can correct mutations or control genes using Cas9. These methods are important annotating genomic information. The this purpose brought together study. explanation detailed description examined will contribute their field microbiology.

Язык: Английский

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Strengthening Molecular Testing Capacity in Colombia: Challenges and Opportunities

Diagnostic Microbiology and Infectious Disease, Год журнала: 2025, Номер unknown, С. 116716 - 116716

Опубликована: Янв. 1, 2025

Язык: Английский

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Pathogenic Bacteria Rapidly Colonise Sinks of Neonatal Intensive Care Unit: Results of A Prospective Surveillance Study

Journal of Hospital Infection, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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Extracellular Vesicles as Biomarkers in Infectious Diseases

Biology, Год журнала: 2025, Номер 14(2), С. 182 - 182

Опубликована: Фев. 11, 2025

Extracellular vesicles (EVs) are nanosized that secreted by all cells into the extracellular space. EVs involved in cell-to-cell communication and can be found different bodily fluids (bronchoalveolar lavage fluid, sputum, urine), tissues, circulation; composition of reflects physiological condition releasing cell. The ability to use from for minimally invasive detection monitor diseases makes them an attractive target. carry a snapshot cell's internal state, they serve as powerful biomarkers diagnosing diseases. also play role body's immune pathogen responses. Pathogens, such bacteria viruses, exploit enhance their survival spread evade system. Changes number or contents signal presence infection, offering potential avenue developing new diagnostic methods infectious Ongoing research this area aims address current challenges range diseases, including infections There is limited literature on development using existing molecular biology approaches. We aim gap reviewing recent EV-related investigations disease studies.

Язык: Английский

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Integrated analysis of the microbiome, culturable strains, and substrate microenvironment reveals significant shifts in bacterial community of Angelica sinensis from fresh roots to medicinal slices

Biologia, Год журнала: 2025, Номер unknown

Опубликована: Фев. 15, 2025

Язык: Английский

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Harnessing advanced molecular diagnostics and bioinformatics to ascertain antimicrobial resistance in ESKAPE organisms

The Microbe, Год журнала: 2025, Номер 7, С. 100316 - 100316

Опубликована: Апрель 1, 2025

Язык: Английский

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Shedding Light on H. pylori Detection: A Fusion Protein Approach Unveiled through LIPS Method

Process Biochemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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Healthcare-Associated Infections: The Role of Microbial and Environmental Factors in Infection Control—A Narrative Review

Infectious Diseases and Therapy, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Язык: Английский

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Efficient on-chip electrochemical lysing of foodborne bacteria for longer DNA strands with high yield for molecular analysis

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 10, 2025

Язык: Английский

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Clinical and microbiological profile of patients with fungal keratitis demonstrating unusual yeast-like structures in potassium hydroxide with calcofluor white preparation of corneal scraping

Journal of Ophthalmic Inflammation and Infection, Год журнала: 2025, Номер 15(1)

Опубликована: Май 26, 2025

Язык: Английский

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