Mosquito-independent milk-associated transmission of zoonotic Wesselsbron virus in sheep DOI Creative Commons

Marta Zimoch,

Llorenç Grau‐Roma, Matthias Liniger

и другие.

PLoS Pathogens, Год журнала: 2024, Номер 20(12), С. e1012751 - e1012751

Опубликована: Дек. 9, 2024

Wesselsbron virus (WSLV) is a zoonotic, mosquito-borne orthoflavivirus endemic to sub-Saharan Africa, causing abortions and stillbirths in small ruminants. The life cycle of WSLV involves Aedes mosquitoes various wildlife domestic animals. Seminal studies the 1950s have shown zoonotic potential WSLV, notably accidental infections laboratory workers exposed infected material. More recent epidemiological suggest emergence clade I strains peri-domestic rural areas western eastern Africa. pathobiology unknown no isolate available. To address these gaps, we generated recombinant SA999 infectious clone (rSA999) by reverse genetics. Subsequently, lactating ewes were inoculated intravenously with rSA999 strain or II SAH177 insect-free biocontainment stables. Inoculated developed fever, viremia, showed high levels viral RNA at mucosal surfaces, elevated titers milk. Milk production was reduced, which directly affected growth lambs, particularly within group. higher their milk each group transmitted infection prolonged secretion. All animals produced antibody cross-neutralizing activity against both strains. Histopathology blood biochemistry analysis indicated liver damage associated necrotizing hepatitis lesions active replication some cases, more pronounced Notably, only SAH177-infected exhibited consistent meningoencephalitis, suggesting that are neurotropic hepatotropic. These findings demonstrate previously unrecognized mode vector-free transmission raises significant concerns for public animal health.

Язык: Английский

African Swine Fever Virus: A Review DOI Creative Commons

Zhaoyao Li,

Wenxian Chen, Zilong Qiu

и другие.

Life, Год журнала: 2022, Номер 12(8), С. 1255 - 1255

Опубликована: Авг. 17, 2022

African swine fever (ASF) is a viral disease with high fatality rate in both domestic pigs and wild boars. ASF has greatly challenged pig-raising countries also negatively impacted regional national trade of pork products. To date, spread throughout Africa, Europe, Asia. The development safe effective vaccines urgently required for the control outbreaks. virus (ASFV), causative agent ASF, large genome complex structure. functions nearly half its genes still remain to be explored. Knowledge on structure function ASFV proteins, mechanism underlying infection immunity, identification major immunogenicity will contribute an vaccine. In this context, paper reviews available knowledge structure, replication, protein function, virulence genes, immune evasion, inactivation, vaccines, control, diagnosis ASFV.

Язык: Английский

Процитировано

109
Mosquito-independent milk-associated transmission of zoonotic Wesselsbron virus in sheep DOI Creative Commons

Marta Zimoch,

Llorenç Grau‐Roma, Matthias Liniger

и другие.

PLoS Pathogens, Год журнала: 2024, Номер 20(12), С. e1012751 - e1012751

Опубликована: Дек. 9, 2024

Wesselsbron virus (WSLV) is a zoonotic, mosquito-borne orthoflavivirus endemic to sub-Saharan Africa, causing abortions and stillbirths in small ruminants. The life cycle of WSLV involves Aedes mosquitoes various wildlife domestic animals. Seminal studies the 1950s have shown zoonotic potential WSLV, notably accidental infections laboratory workers exposed infected material. More recent epidemiological suggest emergence clade I strains peri-domestic rural areas western eastern Africa. pathobiology unknown no isolate available. To address these gaps, we generated recombinant SA999 infectious clone (rSA999) by reverse genetics. Subsequently, lactating ewes were inoculated intravenously with rSA999 strain or II SAH177 insect-free biocontainment stables. Inoculated developed fever, viremia, showed high levels viral RNA at mucosal surfaces, elevated titers milk. Milk production was reduced, which directly affected growth lambs, particularly within group. higher their milk each group transmitted infection prolonged secretion. All animals produced antibody cross-neutralizing activity against both strains. Histopathology blood biochemistry analysis indicated liver damage associated necrotizing hepatitis lesions active replication some cases, more pronounced Notably, only SAH177-infected exhibited consistent meningoencephalitis, suggesting that are neurotropic hepatotropic. These findings demonstrate previously unrecognized mode vector-free transmission raises significant concerns for public animal health.

Язык: Английский

Процитировано

0