Recent advances in virulence of a broad host range plant pathogen Sclerotinia sclerotiorum: a mini-review
Yangyi Zhu,
Chenghong Wu,
Y.P. Deng
и другие.
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Июнь 19, 2024
Sclerotinia
sclerotiorum
is
a
typical
necrotrophic
plant
pathogenic
fungus,
which
has
wide
host
range
and
can
cause
variety
of
diseases,
leading
to
serious
loss
agricultural
production
around
the
world.
It
difficult
control
completely
eliminate
characteristics,
chemical
methods
not
ideal.
Therefore,
it
very
important
know
mechanism
S.
for
improving
living
environment,
relieving
pressure
promoting
economic
development.
In
this
paper,
life
cycle
introduced
understand
whole
process
infection.
Through
analysis
mechanism,
paper
summarized
reported
content,
mainly
focused
on
oxalic
acid,
cell
wall
degrading
enzyme
effector
protein
in
infection
its
mechanism.
Besides,
recent
studies
virulence-related
genes
have
been
paper.
According
analysis,
those
were
related
growth
development
hypha
appressorium,
signaling
regulatory
factors
so
on,
further
influence
ability
infect
critically.
The
application
host-induced
gene
silencing
(HIGS)is
considered
as
potential
effective
tool
various
fungi
crops,
provides
an
reference
study
pathogenesis
green
.
Язык: Английский
Deciphering Cargo Contents in Extracellular Vesicles of Candida haemulonii var. vulnera
Computational and Structural Biotechnology Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Trans-Kingdom sRNA Silencing in Sclerotinia sclerotiorum for Crop Fungal Disease Management
Pathogens,
Год журнала:
2025,
Номер
14(4), С. 398 - 398
Опубликована: Апрель 21, 2025
Sclerotinia
sclerotiorum
is
a
globally
widespread
and
vast
destructive
plant
pathogenic
fungus
that
causes
significant
yield
losses
in
crops.
Due
to
the
lack
of
effective
resistant
germplasm
resources,
control
diseases
caused
by
S.
largely
relies
on
chemical
fungicides.
However,
excessive
use
these
chemicals
not
only
environmental
concerns
but
also
leads
increased
development
resistance
sclerotiorum.
In
contrast,
trans-kingdom
sRNA
silencing-based
technologies,
such
as
host-induced
gene
silencing
(HIGS)
spray-induced
(SIGS),
offer
novel,
effective,
environmentally
friendly
methods
for
management
infection.
This
review
summarizes
recent
advances
identification
genes,
target
selection,
categories,
application
RNA
interference
(RNAi)
technologies
targeting
this
pathogen.
Although
some
challenges,
including
off-target
effects
efficiency
external
uptake,
exist,
findings
have
proposed
solutions
further
improvement.
Combined
with
latest
developments
CRISPR/Cas
editing
other
RNAi
has
potential
become
crucial
tool
sclerotinia
stem
rot
(SSR),
mitigating
impact
crop
production.
Язык: Английский
LRR Receptor-like Protein in Rapeseed Confers Resistance to Sclerotinia sclerotiorum Infection via a Conserved SsNEP2 Peptide
Chenghuizi Yang,
Weiping Zhong,
Wei Li
и другие.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(10), С. 4569 - 4569
Опубликована: Май 10, 2025
Brassica
napus
is
one
of
the
most
extensively
cultivated
oilseed
crops
in
China,
but
its
yield
significantly
impacted
by
stem
rot
caused
Sclerotinia
sclerotiorum.
Receptor-like
proteins
(RLPs)
and
receptor-like
kinases
(RLKs)
play
essential
roles
plant-pathogen
interactions;
however,
their
regulatory
mechanisms
remain
largely
unknown
B.
napus.
In
this
study,
we
investigated
function
leucine-rich
repeat
protein
BnaRLP-G13-1
immunity.
Previous
observations
indicated
that
plants
expressing
exhibited
enhanced
resistance
to
We
hypothesized
mediates
pathogen
recognition
immune
signaling.
To
test
this,
employed
mitogen-activated
kinase
(MAPK)
activity
assays,
transgenic
overexpression
analyses,
infection
assays.
Our
results
demonstrated
recognizes
conserved
necrosis-
ethylene-inducing
peptide
Ssnlp24SsNEP2
derived
from
S.
sclerotiorum,
triggering
MAPK
cascades
subsequent
responses.
Furthermore,
interaction
studies
revealed
physically
interacts
with
BnaSOBIR1,
which
for
full
antifungal
defense
activation.
These
elucidate
molecular
basis
BnaRLP-G13-1-mediated
immunity,
providing
insights
into
improving
disease
crops.
Язык: Английский
The Sclerotinia sclerotiorum ADP-Ribosylation Factor 6 Plays an Essential Role in Abiotic Stress Response and Fungal Virulence to Host Plants
Опубликована: Ноя. 20, 2023
The
ADP-ribosylation
factor
6
(Arf6),
as
the
only
member
of
Arf
family
III
protein,
has
been
extensively
studied
for
its
diverse
biological
functions
in
animals.
Previously,
Arf6
protein
Magnaporthe
oryzae
was
found
to
be
crucial
endocytosis
and
polarity
establishment
during
asexual
development.
However,
role
remains
unclear
Sclerotinia
sclerotiorum.
Here,
we
identified
characterized
SsArf6
S.
sclerotiorum
using
a
reverse
genetic
approach.
Deletion
impaired
hyphal
growth
development,
produced
more
branches.
Interestingly,
knockout
enhanced
tolerance
oxidative
stress
but
sensitivity
osmotic
stress,
increased
melanin
accumulation,
indicative
different
roles
various
responses.
Moreover,
appressorium
formation
severely
impaired,
fungal
virulence
host
plants
significantly
reduced.
Overall,
our
findings
demonstrate
essential
responses,
formation,
plants.
Язык: Английский
The Sclerotinia sclerotiorum ADP-Ribosylation Factor 6 Plays an Essential Role in Abiotic Stress Response and Fungal Virulence to Host Plants
Journal of Fungi,
Год журнала:
2023,
Номер
10(1), С. 12 - 12
Опубликована: Дек. 25, 2023
The
ADP-ribosylation
factor
6
(Arf6),
as
the
only
member
of
Arf
family
III
protein,
has
been
extensively
studied
for
its
diverse
biological
functions
in
animals.
Previously,
Arf6
protein
Magnaporthe
oryzae
was
found
to
be
crucial
endocytosis
and
polarity
establishment
during
asexual
development.
However,
role
remains
unclear
S.
sclerotiorum.
Here,
we
identified
characterized
SsArf6
sclerotiorum
using
a
reverse
genetic
approach.
Deletion
impaired
hyphal
growth
development
produced
more
branches.
Interestingly,
knockout
resulted
an
augmented
tolerance
towards
oxidative
stress,
increased
sensitivity
osmotic
indicative
different
roles
various
stress
responses.
Simultaneously,
deletion
led
elevation
melanin
accumulation.
Moreover,
appressorium
formation
severely
impaired,
fungal
virulence
host
plants
significantly
reduced.
Overall,
our
findings
demonstrate
essential
development,
responses,
formation,
plants.
Язык: Английский
Deciphering Cargo Contents in Extracellular Vesicles ofCandida haemuloniivar.vulnera
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 13, 2024
ABSTRACT
Candida
haemulonii
comprises
a
group
of
pathogenic
fungi
known
for
their
resistance
to
primary
antifungal
treatments.
Infections
caused
by
these
pathogens
present
substantial
challenges
due
the
difficulties
in
accurate
identification.
Extracellular
vesicles
(EVs)
released
play
critical
role
pathogen-host
interaction,
potentially
influencing
and
virulence.
Previous
research
our
indicates
that
EVs
contain
immunogenic
particles
capable
impacting
host’s
immune
response.
Understanding
composition
is
crucial
elucidating
mechanisms
underlying
virulence
C.
var.
vulnera
.
This
study
aims
investigate
contents
from
using
proteomic
microRNA
sequencing
tools,
providing
insights
into
adaptation,
survival,
progression
infections.
Our
findings
reveal
key
proteins
transported
EVs,
including
BMH1,
TEF1,
CDC19,
PDC11.
These
are
involved
various
cellular
processes,
such
as
alteration
cell
wall
structure,
biofilm
formation,
facilitation
morphological
changes,
among
others.
Additionally,
we
observed
miRNA-like
molecules
within
linked
electron
transport
chain
regulation
citric
acid
cycle,
which
metabolic
processes
associated
with
factors
rapid
adaptation
diverse
hosts
or
environments.
In
this
context,
provide
novel
perspective
on
fungal
highlighting
potential
targets
therapies.
Therefore,
may
reflect
expression
levels
regulatory
pathogenicity,
IMPORTANCE
The
complex
holds
clinical
significance
its
notable
conventional
therapies
inherent
specific
focuses
cargo
fungi,
an
essential
interactions,
pathogenicity.
can
modulate
Proteomic
analyses
have
identified
miRNAs
adjustment,
modulation
cytoplasmic
functions.
components
offers
underscoring
therapeutic
intervention.
By
,
enhances
understanding
pathogenicity
emerging
yeast.
Язык: Английский
Transcriptomic and protein analysis of Trametes versicolor interacting with a Hypholoma fasciculare mycelium foraging in soil
Fungal ecology,
Год журнала:
2024,
Номер
72, С. 101385 - 101385
Опубликована: Сен. 27, 2024
Язык: Английский
Exploring Protein Functions of Gut Bacteriome and Mycobiome in Thai Infants Associated with Atopic Dermatitis Through Metaproteomic and Host Interaction Analysis
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13533 - 13533
Опубликована: Дек. 18, 2024
Atopic
dermatitis
(AD),
a
prevalent
allergic
skin
condition
in
children,
has
been
closely
associated
with
imbalances
the
gut
microbiome.
To
investigate
these
microbial
alterations
and
their
functional
implications,
we
investigated
protein
expression,
functions
interactions
of
bacteriome
mycobiome
as
well
human
proteome
Thai
infants
AD
using
integrative
metaproteomic
host
interaction
analysis.
As
observed,
probiotic
species,
such
Lactobacillus
acidophilus
Bacteroides
salyersiae,
were
reduced
abundance
group
while
key
pathogenic
bacteria
fungi,
Streptococcus
constellatus
Penicillium
chrysogenum,
increased
abundance.
Additionally,
analysis
expressed
proteins
was
enriched
response
to
stress
DNA
repair
ribosome
biogenesis-related
processes
group,
potentially
reactive
oxygen
species
(ROS),
intestinal
inflammation,
fungal
growth
dysbiosis.
Further,
protein–protein
(PPIs)
network
incorporating
revealed
10
signature
related
immune
system
AD.
Our
findings
propose
between
microbes
infants.
our
knowledge,
this
study
serves
first
framework
for
monitoring
bacteriome–mycobiome–human
studies
other
diseases
Язык: Английский