Bacteriophage therapy for multidrug-resistant infections: current technologies and therapeutic approaches
Journal of Clinical Investigation,
Год журнала:
2025,
Номер
135(5)
Опубликована: Март 2, 2025
Bacteriophage
(phage)
therapy
has
emerged
as
a
promising
solution
to
combat
the
growing
crisis
of
multidrug-resistant
(MDR)
infections.
There
are
several
international
centers
actively
engaged
in
implementation
phage
therapy,
and
recent
case
series
have
reported
encouraging
success
rates
patients
receiving
personalized,
compassionate
for
difficult-to-treat
Nonetheless,
substantial
hurdles
remain
way
more
widespread
adoption
consistent
success.
This
Review
offers
comprehensive
overview
current
technologies
therapeutic
approaches.
We
first
delineate
common
steps
development,
from
bank
establishment
clinical
administration,
examine
spectrum
approaches,
personalized
fixed
cocktails.
Using
framework
conventional
drug
development
pipeline,
we
then
identify
critical
knowledge
gaps
areas
such
cocktail
design,
formulation,
pharmacology,
trial
design.
conclude
that,
while
holds
promise,
structured
pipeline
sustained
government
support
crucial
MDR
Язык: Английский
Phage therapy: an alternative treatment modality for MDR bacterial infections
Infectious Diseases,
Год журнала:
2024,
Номер
56(10), С. 785 - 817
Опубликована: Июль 17, 2024
The
increasing
global
incidence
of
multidrug-resistant
(MDR)
bacterial
infections
threatens
public
health
and
compromises
various
aspects
modern
medicine.
Recognising
the
urgency
this
issue,
World
Health
Organisation
has
prioritised
development
novel
antimicrobials
to
combat
ESKAPEE
pathogens.
Comprising
Язык: Английский
Non-antibiotic therapies for multidrug-resistant gastrointestinal infections: an overview of the use of probiotics, natural compounds, and bacteriophages
Frontiers in Antibiotics,
Год журнала:
2025,
Номер
4
Опубликована: Май 6, 2025
The
worldwide
increasing
frequency
and
severity
of
multidrug-resistant
gastrointestinal
(MDR-GI)
infections
not
only
raises
awareness
the
debilities
conventional
antibiotic
treatments
but
also
highlights
demand
for
alternative
interventions.
One
these
alternatives
is
probiotics,
harmless
bacteria
that
compete
with
pathogenic
species,
which
have
been
considered
beneficial
due
to
their
therapeutic
potential
since
they
strengthen
mucosal
barrier
modulate
host
immune
response.
Other
natural
compounds
(e.g.,
polyphenols,
flavonoids,
essential
oils)
present
diverse
antimicrobial
mechanisms,
are
promising
mitigate
resistant
pathogens.
Finally,
bacteriophages,
viruses
target
specific
bacteria,
constitute
a
precise
approach
in
MDR
lysed
or
disrupted
by
biofilms
formed
during
colonization
without
compromising
normal
gut
microbiome.
Therefore,
manuscript
provides
an
integrated
perspective
on
non-antibiotic
therapies
manage
MDR-GI
infections;
this
purpose,
it
covers
aspects
such
as
action
current
clinical
applications,
challenges
limit
broader
application
practice.
combining
approaches
personalizing
infection
adjusted
patients'
microbiome
profiles
discussed,
aiming
enhance
efficacy
reduce
resistance
risks.
importance
continued
research
development
optimize
debated,
addressing
need
surpass
regulatory
barriers
conducting
large-scale
trials
establish
safety
alternatives.
This
overview
knowledge
contributes
ongoing
efforts
develop
sustainable
strategies
combat
global
burden
resistance.
Язык: Английский
Characterization and Genomic Analysis of a New Bacteriophage Klebsiella pneumoniae CTF-1 from Turkey
Опубликована: Май 14, 2025
Abstract
Background
Klebsiella
pneumoniae
is
a
clinically
important
pathogen
causing
respiratory
tract
infections,
pneumonia,
wound
urinary
infections
and
sepsis.
It
on
the
priority
list
of
World
Health
Organization
(WHO)
due
to
resistant
it
causes.
The
K.
CTF1
phage
novel
lytic
with
highest
overall
similarity
82.19%
against
isolated
from
clinical
infections.
In
this
study,
whole
genome
analysis
was
conducted.
Results
has
linear
double-stranded
DNA
40,841
bp
long
53.1%
GC
content.
Totally
44
protein
coding
genes
were
identified
in
genome.
31
which
assigned
functions
as
replication,
packaging,
structural
proteins
or
lysis.
CTF-1
member
Przondovirus
genus
within
Caudovirales
order.
objective
research
explore
effectiveness
CTF-I
bacteriophage
isolate
panresistant
K.
pneumoniae
isolates.
effective
22
(88%)
25
strains.
optimum
efficacy
37–45°C,
optimal
pH
between
4–9.
latent
time
cycle
around
40
min
burst
size
92
PFU/mL.
Conclusions
an
excellent
candidate
for
therapy
its
high
activity
Klebsiella
lack
antibiotic
resistance
genes,
toxins,
virulence
factors
integrase
genes.
Язык: Английский
Lytic Spectra of Tailed Bacteriophages: A Systematic Review and Meta-Analysis
Viruses,
Год журнала:
2024,
Номер
16(12), С. 1879 - 1879
Опубликована: Дек. 4, 2024
As
natural
predators
of
bacteria,
tailed
bacteriophages
can
be
used
in
biocontrol
applications,
including
antimicrobial
therapy.
Also,
phage
lysis
is
a
detrimental
factor
technological
processes
based
on
bacterial
growth
and
metabolism.
The
spectrum
bacteria
interact
with
known
as
the
host
range.
Phage
science
produced
vast
amount
range
data.
However,
there
has
been
no
attempt
to
analyse
these
data
from
viewpoint
modern
taxonomy.
Here,
we
performed
meta-analysis
spotting
plaquing
obtained
strains
production
species.
main
metric
our
study
was
value
calculated
ratio
lysed
number
tested
strains.
We
found
boundary
between
narrow
broad
ranges
phages
taken
whole.
Family-level
groups
strictly
lytic
had
significantly
different
median
values
0.18
(Drexlerviridae)
0.70
(Herelleviridae).
In
Escherichia
coli
phages,
were
associated
decreased
efficiency
plating.
Bacteriophage
morphology,
genome
size,
tRNA-coding
genes
genomes
did
not
correlate
values.
From
perspective
species,
varied
0.04
infecting
Acinetobacter
baumannii
0.73
Staphylococcus
aureus
phages.
Taken
together,
results
imply
that
taxonomy
their
hosts
predictive
intraspecies
ranges.
Язык: Английский
Evaluating the effectiveness and safety of a novel phage cocktail as a biocontrol of Salmonella in biofilm, food products, and broiler chicken
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 28, 2024
is
a
foodborne
pathogen
of
animal
and
public
health
significance.
Considering
the
disadvantages
antibiotics
or
chemical
preservatives
traditionally
used
to
eliminate
this
pathogen,
attention
has
shifted,
in
recent
years,
toward
biocontrol
agents
such
as
bacteriophages,
either
separately
combination
prevent
food
contamination.
However,
extensive
use
phage-based
industry
requires
further
studies
ensure
their
safety
efficacy.
In
present
study,
we
investigated
effectiveness
phage
cocktail,
cocktail
comprising
three
pre-characterized
Язык: Английский
In Vitro Susceptibility of Clinical and Carrier Strains of Staphylococcus aureus to STAFAL® Phage Preparation
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12885 - 12885
Опубликована: Ноя. 29, 2024
The
treatment
of
infections
caused
by
Язык: Английский