Genomic surveillance of SARS-CoV-2 variants using pooled WGS DOI Creative Commons
In Ho Park, Yoonjung Kim, Min Hyuk Choi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 22, 2025

This study presents the development and validation of a genomic surveillance strategy using Whole Genome Sequencing (WGS) on normalized pooled samples to detect monitor SARS-CoV-2 variants. A bioinformatics pipeline was designed specifically for analyzing WGS data validated simulated datasets, reference materials, clinical collected during key periods Delta Omicron variant emergence. The approach evaluated its accuracy in estimating abundance at both Phylogenetic Assignment Named Global Outbreak (PANGO) lineage level World Health Organization (WHO) level. From simulation method achieved an overall sensitivity 99.1% positive predictive value (PPV) 99.9% detecting variants WHO At PANGO level, it 82.8% PPV 77.4% when predicted considered accurate if shared more than 90% markers with any true present sample. estimation further materials. Analysis showed results consistent national epidemiological trends, particularly emergence Korea. WGS-based offers scalable economical solution monitoring variants, providing public health authorities valuable tool tracking pandemic dynamics enabling timely responses.

Язык: Английский

The resurgence of monkeypox clade Ib: a global health emergency and concern DOI
Suresh Kumar, Gunasekaran Subramaniam,

K. J. Senthil Kumar

и другие.

Archives of Virology, Год журнала: 2025, Номер 170(4)

Опубликована: Март 21, 2025

Язык: Английский

Процитировано

1
Anti-Orthopoxvirus Activity of Amantadine and Rimantadine Derivatives—In Vitro Testing and Molecular Modeling DOI Creative Commons

Ivan A. Moskalev,

Е. А. Akishina, Е. А. Дикусар

и другие.

Chemistry, Год журнала: 2025, Номер 7(2), С. 34 - 34

Опубликована: Март 1, 2025

In 2022, the number of mpox cases spiked worldwide, leading to a surge in scientific research on members Orthopoxvirus genus and discovery new compounds exhibiting anti-orthopoxvirus activity. This work is devoted synthesis containing an adamantane fragment evaluation their activity against vaccinia virus, offering possible mechanism antiviral action synthesized agents. Among all studied derivatives, three (2, 4, 12) were found demonstrate highest activity, with most promising compound 2 (N-(adamantan-1-yl)isonicotinamide) having lowest toxicity level selectivity index (SI) 115. The pharmacophoric profiles these are similar profile tecovirimat, inhibitor membrane viral protein p37. Analysis results molecular modeling suggests that investigated can inhibit virus by suppressing phospholipase

Язык: Английский

Процитировано

0
In silico evidence of monkeypox F14 as a ligand for the human TLR1/2 dimer DOI Creative Commons
Ankita Chakraborty, Nabarun Chandra Das, Parth Sarthi Sen Gupta

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 17, 2025

Recent emergence of zoonotic monkeypox virus (Mpox) in human has triggered the virologists to develop plausible preventive measures. Hitherto, our understanding on mechanism immunopathogenesis Mpox infection is elusive. However, available experimental evidences suggest induction inflammation as main cause pathogenesis. Toll-like receptors (TLRs) are critical initiating and modulating host immune response pathogens. Inflammatory responses observed various poxvirus infections have, fact, been shown be mediated through TLR activation. Therefore, by silico approaches, this study seeks identify antigen(s) (MAg) that most likely interact with cell-surface TLRs. The proteomics data UniProt database contain 174 protein sequences, among which 105 immunoreactive proteins were modeled for 3D structure examined comparative protein-protein interactions TLRs molecular docking dynamics simulation. F14, an 8.28 kDa infective Mpox, was found exhibit strong binding affinity (ΔG=-12.5 Kcal mol-1) TLR1/2 dimer form a compact thermodynamically stable complex. Interestingly, significant level conformational change also both F14 TLR6 while forming F14-TLR1/2 Based these we propose putative ligand initiate proinflammatory signaling Mpox-infected host.

Язык: Английский

Процитировано

0
MPOX unveiled: Global epidemiology, treatment advances, and prevention strategies DOI Creative Commons
Anna Bogacka,

Agnieszka Wroczyńska,

Weronika Rymer

и другие.

One Health, Год журнала: 2025, Номер unknown, С. 101030 - 101030

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Genomic surveillance of SARS-CoV-2 variants using pooled WGS DOI Creative Commons
In Ho Park, Yoonjung Kim, Min Hyuk Choi

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 22, 2025

This study presents the development and validation of a genomic surveillance strategy using Whole Genome Sequencing (WGS) on normalized pooled samples to detect monitor SARS-CoV-2 variants. A bioinformatics pipeline was designed specifically for analyzing WGS data validated simulated datasets, reference materials, clinical collected during key periods Delta Omicron variant emergence. The approach evaluated its accuracy in estimating abundance at both Phylogenetic Assignment Named Global Outbreak (PANGO) lineage level World Health Organization (WHO) level. From simulation method achieved an overall sensitivity 99.1% positive predictive value (PPV) 99.9% detecting variants WHO At PANGO level, it 82.8% PPV 77.4% when predicted considered accurate if shared more than 90% markers with any true present sample. estimation further materials. Analysis showed results consistent national epidemiological trends, particularly emergence Korea. WGS-based offers scalable economical solution monitoring variants, providing public health authorities valuable tool tracking pandemic dynamics enabling timely responses.

Язык: Английский

Процитировано

0