The potential of plant antimicrobial peptides for crop protection against diseases DOI Creative Commons
Anna S. Barashkova, Е. А. Рогожин

Plant Protection News, Год журнала: 2023, Номер 106(3), С. 120 - 136

Опубликована: Дек. 13, 2023

Antimicrobial peptides (AMPs) are the most important components of plant innate immunity to environmental stress factors and one ancient tools defense system. Most them synthesized as constitutive immunity, but there also inducible forms belonging “pathogenesis-related proteins” (PR-proteins from classes 12, 13 14). This review provides characteristics primary three-dimensional structures main families AMPs. The relationship between types spatial arrangement polypeptide chain is drawn. functional analysis AMPs presented by data on spectrum quantitative level activities against a number economically significant fungal bacterial phytopathogens, related biological effects indicated. Additionally, current information provided molecular mechanisms antimicrobial action based defensins studied structural group. In conclusion, aspects modes for microorganisms considered, basis which variant classification these molecules proposed. Based data, prospects their use biopesticides protection pathogens were assessed.

Язык: Английский

Plant non-specific lipid transfer proteins: An overview DOI Creative Commons

Khawla Missaoui,

Zulema González‐Klein, Diego Pazos‐Castro

и другие.

Plant Physiology and Biochemistry, Год журнала: 2021, Номер 171, С. 115 - 127

Опубликована: Дек. 30, 2021

Plant non-specific lipid transfer proteins (nsLTPs) are usually defined as small, basic proteins, with a wide distribution in all orders of higher plants. Structurally, nsLTPs contain conserved motif eight cysteines, linked by four disulphide bonds, and hydrophobic cavity which the ligand is housed. This structure confers stability enhances ability to bind transport variety molecules. Their highly structural resemblance but low sequence identity reflects ligands they can carry, well broad biological functions to, such membrane stabilization, cell wall organization signal transduction. In addition, have also been described essential resistance biotic abiotic stresses, plant growth development, seed germination. Hence, there growing interest this family for their critical roles development many unresolved questions that need be clarified, regarding subcellular localization, capacity, expression profile, function, evolution.

Язык: Английский

Процитировано

75
Food Allergens of Plant Origin DOI Creative Commons
Yuzhu Zhang, Huilian Che, Caiming Li

и другие.

Foods, Год журнала: 2023, Номер 12(11), С. 2232 - 2232

Опубликована: Июнь 1, 2023

This review presents an update on the physical, chemical, and biological properties of food allergens in plant sources, focusing few protein families that contribute to multiple from different species recently found contain allergens. The structures structural components allergen may provide further directions for discovering new Answers as what makes some proteins are still elusive. Factors be considered mitigating include abundance a food, property short stretches sequence constitute linear IgE binding epitopes, protein, its stability heat digestion, matrix is in, antimicrobial activity microbial flora human gastrointestinal tract. Additionally, recent data suggest widely used techniques mapping epitopes need improved by incorporating positive controls, methodologies conformational developed.

Язык: Английский

Процитировано

15
Lipid transfer protein VAS inhibits the hypersensitive response via reactive oxygen species signaling in Nicotiana benthamiana DOI Creative Commons

Rina Koyama,

Akira A. Suzuki,

Kouhei Ohnishi

и другие.

Journal of Experimental Botany, Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

Lipid transfer proteins (LTPs) are small cysteine-rich soluble that affect flower and seed development, cuticular wax deposition, biotic abiotic stress responses. We isolated an LTP-encoding gene homologous to LTPVAS in Nicotiana benthamiana designated it LTP-VASCULAR TISSUE SIZE (NbLTPVAS). This was expressed seeds, leaves, roots, stems. Additionally, NbLTPVAS expression induced by hypersensitive response (HR)-inducing agents. Cell death accelerated the phytopathogenic bacterial population decreased significantly NbLTPVAS-silenced plants infected with incompatible Ralstonia solanacearum strain 8107. The of HR marker hin1 markedly R. 8107, indicative acceleration HR. cell also promoted Agrobacterium-mediated HR-inducing including INF1, AvrA, PopP1. Excessive production reactive oxygen species (ROS) detected plants. NbrbohB (encoding a ROS-generating enzyme) increased plants, but antioxidant enzyme-encoding genes NbSOD NbAPX decreased. silencing both adversely affected induction. Moreover, secreted into intercellular washing fluid. transient full-length genes, attenuated ROS production, suppressed induction death. is first functional analysis plant-microbe interactions. Our study provides novel insights role as negative regulator via homeostasis N. benthamiana.

Язык: Английский

Процитировано

0
Lipid Transfer Proteins (LTPs)—Structure, Diversity and Roles beyond Antimicrobial Activity DOI Creative Commons
Vinícius Costa Amador, Carlos André dos Santos-Silva,

Lívia Maria Batista Vilela

и другие.

Antibiotics, Год журнала: 2021, Номер 10(11), С. 1281 - 1281

Опубликована: Окт. 21, 2021

Lipid transfer proteins (LTPs) are among the most promising plant-exclusive antimicrobial peptides (AMPs). They figure challenging AMPs from point of view their structural diversity, functions and biotechnological applications. This review presents a current picture LTP research, addressing not only structural, evolutionary further predicted functional aspects. Traditionally, LTPs have been identified by direct isolation biochemical techniques, whereas omics data bioinformatics deserve special attention for potential to bring new insights. In this context, possible revealing that actually multipurpose, with many additional roles. Despite some challenges due toxicity allergenicity LTPs, systematic search in patent databases, indicate perspectives use human health also plant defense.

Язык: Английский

Процитировано

29
Endophytic entomopathogenic fungi enhance plant immune responses against tomato leafminer DOI

S M Ghafari,

Javad Karimi, Monireh Cheniany

и другие.

Journal of Invertebrate Pathology, Год журнала: 2025, Номер unknown, С. 108270 - 108270

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Structural analysis of arabinoxylan and its effect on beer foam: A comparison of three arabinoxylans with different viscosity DOI
Wen Li,

Jingyan Lu,

Yundong Ju

и другие.

Food Hydrocolloids, Год журнала: 2025, Номер unknown, С. 111117 - 111117

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Lipid transfer proteins: structure, classification and prospects of genetic engineering for improved disease resistance in plants DOI

Aneela Iqbal,

Raham Sher Khan,

Daud Ali Shah

и другие.

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2023, Номер 153(1), С. 3 - 17

Опубликована: Янв. 14, 2023

Язык: Английский

Процитировано

10
Towards Marker-Assisted Breeding for Black Rot Bunch Resistance: Identification of a Major QTL in the Grapevine Cultivar ‘Merzling’ DOI Open Access
Paola Bettinelli, Daniela Nicolini, Laura Costantini

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 3568 - 3568

Опубликована: Фев. 10, 2023

Black rot (BR), caused by Guignardia bidwellii, is an emergent fungal disease threatening viticulture and affecting several mildew-tolerant varieties. However, its genetic bases are not fully dissected yet. For this purpose, a segregating population derived from the cross ‘Merzling’ (hybrid, resistant) × ‘Teroldego’ (V. vinifera, susceptible) was evaluated for BR resistance at shoot bunch level. The progeny genotyped with GrapeReSeq Illumina 20K SNPchip, 7175 SNPs were combined 194 SSRs to generate high-density linkage map of 1677 cM. QTL analysis based on trials confirmed previously identified Resistance bidwellii (Rgb)1 locus chromosome 14, which explained up 29.2% phenotypic variance, reducing genomic interval 2.4 0.7 Mb. Upstream Rgb1, study revealed new explaining 79.9% variance resistance, designated Rgb3. physical region encompassing two QTLs does underlie annotated (R)-genes. Rgb1 resulted enriched in genes belonging phloem dynamics mitochondrial proton transfer, while Rgb3 presented cluster pathogenesis-related Germin-like protein genes, promoters programmed cell death. These outcomes suggest strong involvement oxidative burst occlusion mechanisms provide molecular tools grapevine marker-assisted breeding.

Язык: Английский

Процитировано

9
Molecular Insights into the Role of Cysteine-Rich Peptides in Induced Resistance to Fusarium oxysporum Infection in Tomato Based on Transcriptome Profiling DOI Open Access
Marina P. Slezina, Е. А. Истомина,

Tatyana V. Korostyleva

и другие.

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(11), С. 5741 - 5741

Опубликована: Май 27, 2021

Cysteine-rich peptides (CRPs) play an important role in plant physiology. However, their resistance induced by biogenic elicitors remains poorly understood. Using whole-genome transcriptome sequencing and our CRP search algorithm, we analyzed the repertoire of CRPs tomato Solanum lycopersicum L. response to Fusarium oxysporum infection from F. sambucinum. We revealed 106 putative transcripts belonging different families antimicrobial (AMPs), signaling (RALFs), with non-defense functions (Major pollen allergen Olea europaea (Ole e 1 6), Maternally Expressed Gene (MEG), Epidermal Patterning Factor (EPF)), as well pathogenesis-related proteins 4 (PR-1 4). discovered a novel type 10-Cys-containing hevein-like AMPs named SlHev1, which was up-regulated both elicitors. Transcript profiling showed that sambucinum changed expression levels overlapping sets genes, suggesting diversification families. non-specific lipid transfer (nsLTPs) snakins mostly contribute plants The involvement function stress reactions also demonstrated. results obtained shed light on mode action immune tomato.

Язык: Английский

Процитировано

21
Genome-Wide Identification and Expression Analysis of nsLTP Gene Family in Rapeseed (Brassica napus) Reveals Their Critical Roles in Biotic and Abiotic Stress Responses DOI Open Access
Yufei Xue, Chunyu Zhang, Rui Shan

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(15), С. 8372 - 8372

Опубликована: Июль 28, 2022

Non-specific lipid transfer proteins (nsLTPs) are small cysteine-rich basic which play essential roles in plant growth, development and abiotic/biotic stress response. However, there is limited information about the nsLTP gene (BnLTP) family rapeseed (Brassica napus). In this study, 283 BnLTP genes were identified rapeseed, distributed randomly 19 chromosomes of rapeseed. Phylogenetic analysis showed that divided into seven groups. Exon/intron structure MEME motifs both remained highly conserved each group. Segmental duplication hybridization rapeseed’s two sub-genomes mainly contributed to expansion family. Various potential cis-elements respond development, biotic/abiotic stresses, phytohormone signals existed promoters. Transcriptome expressed various tissues/organs with different levels also involved response heat, drought, NaCl, cold, IAA ABA as well treatment fungal pathogens (Sclerotinia sclerotiorum Leptosphaeria maculans). The qRT-PCR assay validated results RNA-seq expression top Sclerotinia-responsive genes, BnLTP129 BnLTP161. Moreover, batches BnLTPs might be regulated by BnTT1 BnbZIP67 metabolism or adaptability seed coat embryo This work provides an important basis for further functional study quality improvement resistance.

Язык: Английский

Процитировано

13