Fmoc-FF Nanogel-Mediated Delivery of Doxorubicin and Curcumin in Thyroid Cancer Cells

Pharmaceutics, Год журнала: 2025, Номер 17(2), С. 263 - 263

Опубликована: Фев. 17, 2025

Background: Thyroid cancer (TC) is the most prevalent endocrine malignancy, and categorized into well-differentiated aggressive anaplastic types. Novel therapeutic modalities are needed for TC. Nanomedicine a promising strategy development of precision medicine. In this context, we investigated use nanogels (NGs) to deliver agents with different physicochemical properties, specifically hydrophilic agent doxorubicin (DOX) hydrophobic compound curcumin (CUR), in TC cell lines. Methods: Nα-9-fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) peptide-based NGs loaded DOX CUR were formulated using solvent-switch method. DOX-loaded previously characterized. CUR-loaded characterized through rheology, scanning electron microscopy (SEM), dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), Fourier transform infrared (FT-IR) spectroscopy. Confocal microscopy, q-RT-PCR, ATP lite assays performed evaluate uptake delivery DOX- on Results: exhibited mean diameter approximately 204.3 nm zeta potential -34.6 mV, indicative good stability. vitro release studies revealed sustained profile over 72 h. Functional analyses demonstrated that Fmoc-FF-loaded internalized They primarily localized cytoplasm rather than early endosomes, thereby ensuring intracellular Furthermore, Fmoc-FF reduced nuclear kinetics cells, suggesting reduction dose-limiting toxicity. Comparative similar internalization delayed uptake, highlighting efficacy delivering agents. Conclusions: Overall, data suggest represent diverse properties TC, enhancing their safety warranting further investigation.

Язык: Английский

---

Protective effects of herbal compounds against cyclophosphamide-induced organ toxicity: a pathway-centered approach

Drug and Chemical Toxicology, Год журнала: 2025, Номер unknown, С. 1 - 43

Опубликована: Янв. 23, 2025

Cyclophosphamide is a key component of numerous chemotherapeutic protocols, demonstrating broad-spectrum efficacy against various malignancies and non-cancerous conditions. This review examines CPM's metabolic pathways, therapeutic applications, its resulting organ-specific toxicities. Despite clinical benefits in treating nephrotic syndrome, encephalomyelitis, breast cancer, ovarian other diseases, CPM associated with significant adverse effects on the kidneys, liver, heart, lungs, intestines. The discussion delves into molecular mechanisms underlying these toxicities, highlighting dysregulation signaling including Nrf2, NF-κB, MAPK/ERK, AKT. In addressing challenges, recent studies have identified herbal drugs phytochemicals capable mitigating CPM-induced toxicity. Notable compounds such as cinnamaldehyde, baicalin, quercetin, curcumin demonstrated protective effects. Integrating formulations therapy proposed to enhance patient safety treatment efficacy. underscores influence apoptosis inflammation which lead alterations biomarkers. Phytochemicals may exert by restoring disrupted pathways normalizing altered compilation presented this serves valuable resource for researchers exploring products potential A gap current literature lack trials evaluating that mitigate toxicity vivo. Rigorous are necessary establish cancer treatment. Such research will clarify role natural remedies complementing conventional therapies, ultimately improving outcomes.

Язык: Английский

---

Therapeutic Potential of Plant-Derived Compounds and Plant Extracts in Rheumatoid Arthritis—Comprehensive Review

Antioxidants, Год журнала: 2024, Номер 13(7), С. 775 - 775

Опубликована: Июнь 27, 2024

Rheumatoid arthritis (RA) is a persistent autoimmune disorder that characterized by joint inflammation, discomfort, and impairment. Despite the existence of several therapeutic approaches, their effectiveness often restricted may be linked to unfavorable side effects. Consequently, there has been growing interest in investigating naturally derived compounds as plausible agents for RA disease. The objective this review summarize existing preclinical clinical evidence regarding efficacy extracted plant extracts treatment RA, focusing on anti-inflammatory, anti-oxidative, immunomodulatory properties. Some problems with using natural chemicals are uneven quality commercially available preparations poor bioavailability these compounds. Future investigations should focus improving formulations, conducting thorough trials, exploring different techniques fully utilize intrinsic potential treating RA.

Язык: Английский

---

The Therapeutic Effects of Curcumin on Oral Disease: A Systematic Review

Clinical Pharmacology Advances and Applications, Год журнала: 2025, Номер Volume 17, С. 13 - 24

Опубликована: Фев. 1, 2025

Curcumin is an extract from herbal plants that has been implicated in the treatment of any disease, including oral disease. There are various types curcumin formulation as option therapy. The aim this review to describe mechanism reducing severity, pain score, and lesion size therapeutic effects. This systematic used Preferred Reporting Items for Systematic Review Meta Analysis (PRISMA) guidelines. Databases articles include PubMed, Science Direct, Scopus with inclusion criteria published 2014 2024, full text, English, randomized controlled trial (RCT). present study included 21 RCTs a total 1244 individuals. In study, was most commonly submucous fibrosis, 9 studies demonstrating anti-inflammatory properties inhibits collagenase. All demonstrate produces significant results management remain showed antioxidant, inhibit collagenase, antifungal, wound healing leukoplakia, recurrent aphthous stomatitis (RAS), lichen planus (OLP), denture stomatitis. anti inflammatory, severity lesion, score effects patients disease OSMF, mucositis, RAS, OLP,

Язык: Английский

---

Experimental investigation and thermodynamic modelling of cannabidiol and curcumin in different solvents

Journal of Molecular Liquids, Год журнала: 2024, Номер 410, С. 125511 - 125511

Опубликована: Июль 16, 2024

Cannabidiol (CBD) and curcumin (CUR) are pharmacologically active substances whose therapeutic effect is limited due to their low water solubility. In this study, we investigated solubility in different solvents experimentally using the shake flask method silico COSMO-RS software. We found that CBD very soluble DMSO, DMF, acetone, methanol, diethyl ether, isopropanol, ethanol, 1-butanol, PEG 400, triacetin, triethyl citrate, propylene glycol, hexane, isooctane vegetable oils. CUR acetone 400 slightly insoluble The shows reasonable relative accuracy (measured as absolute percentage error) for predicting of which these compounds soluble.

Язык: Английский

---

Exploring the impact of curcumin on osteoarthritis symptomatology: correlations and insights from a Bulgarian cohort

Folia Medica, Год журнала: 2024, Номер 66(4), С. 461 - 465

Опубликована: Авг. 31, 2024

Introduction : Osteoarthritis is a prevalent degenerative joint disorder associated with pain and functional impairment. Curcumin, natural anti-inflammatory compound, has garnered attention for its potential therapeutic benefits in osteoarthritis management. Aim This study aimed to investigate the correlations between curcumin use, patient characteristics, medication changes health outcomes among patients. The primary hypothesis was that use would correlate positive metrics. Materials methods A cohort of 60 patients enrolled this observational divided into 4 groups according type osteoarthritis. Patient selection criteria included diagnosis consent supplementation. outcome variable metrics measured across three visits. Body mass index, nonsteroidal drugs (NSAIDs) paracetamol were also recorded. Correlation analysis paired t -tests employed examine relationships variables Results analyses revealed weak negative correlation BMI ‘Visit 1’ 2’ ( r =−0.09). NSAIDs exhibited during period =0.17), while displayed =−0.05) changes. Statistically significant observed (mean change =−5.93, p <0.05), aligning prior studies indicating curcumin’s symptom relief. Conclusions provides valuable insights intricate exist curcumin, Despite many limitations, including sample size research duration, our indicates may have impact on symptomatology findings highlight need more studies, larger trials, mechanistic completely understand role

Язык: Английский

---

Comparative Pharmacokinetics and Tissue Distribution of Hexahydrocurcumin Following Intraperitoneal vs Oral Administration in Mice Using LC-MS/MS

ACS Omega, Год журнала: 2024, Номер 9(39), С. 41032 - 41042

Опубликована: Сен. 19, 2024

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine hexahydrocurcumin (HHC) levels in mouse plasma, brain, liver, kidneys using a negative ion mode electrospray ionization (ESI) source. Demonstrating lower limit of quantification (LLOQ) 5 ng/mL, the showed excellent linearity across concentration range 5-500 ng/mL all tested matrices. Precision evaluations reported coefficient variation (CV%) less than 13.19% for both intraday interday measurements, while accuracy ranged from 95.13 105.07% quality control levels. HHC extraction recovery consistently observed between 70.18 93.28%, with CV% deviation 15%. In pharmacokinetic evaluation mice following single intraperitoneal (IP) or oral administration, noncompartment analysis utilized. After IP administration (40 mg/kg),

Язык: Английский

---

Analysing the Composition of Commercial Turmeric Powder: Assessing Contaminants and Its Impacts Curcumin and Water-soluble Vitamins Levels

European Journal of Nutrition & Food Safety, Год журнала: 2024, Номер 16(5), С. 149 - 158

Опубликована: Май 8, 2024

Aims: Turmeric, with its active component curcumin, has garnered global attention for medicinal benefits, including anti-inflammatory and antioxidant properties. This study aimed to analyse turmeric powder obtained from the Greater Accra Metropolis nutrients contaminants. Study Design: Experimental. Place Duration of Study: Entrance Pharmaceuticals, 6 months. Methodology: 22 samples 10 different processing sites open markets were tested using physical chemical methods. HPLC identified ascorbic acid, riboflavin, thiamine, pyridoxine levels. An independent t-test was done compare concentrations these in powdered two sources. Results: Assessment showed no yellow lead salts but 9.1% adulterated chalk, 91% contained metanil yellow. Curcumin (2014.95 vs. 567.79), riboflavin (21.60 1.75), thiamine (14.75 0.65 mg/mL), (9.35 acid (0.00 101.60 mg/mL) significantly higher (p<0.05) processed than market ones. Samples without adulterants had curcumin micronutrient Conclusion: Strengthening monitoring programs is crucial tackling food adulteration concerns.

Язык: Английский

---

Curcumin-Based Molecularly Imprinted Polymer Electropolymerized on Single-Use Graphite Electrode for Dipyridamole Analysis

Molecules, Год журнала: 2024, Номер 29(19), С. 4630 - 4630

Опубликована: Сен. 29, 2024

A new molecularly imprinted polymer (MIP)-based disposable electrochemical sensor for dipyridamole (DIP) determination was obtained. The rapidly prepared by potentiodynamic polymerization on a pencil graphite electrode (PGE) using curcumin (CUR) as functional monomer and DIP template molecule. After the optimization of conditions (pH, monomer–template ratio, scan rate, number cyclic voltammetric cycles applied in electro-polymerization process extraction time molecule) MIP formation, behavior at modified (MIP_PGE) investigated. oxidation took place pH-dependent, irreversible mixed diffusion-adsorption controlled process. Differential pulse voltammetry (DPV) adsorptive stripping differential (AdSDPV) were used to quantify from pharmaceutical tap water samples. Under optimized (Britton–Robinson buffer pH = 3.29), obtained linear ranges 5.00 × 10−8–1.00 10−5 mol/L 10−9–1.00 10−7 DPV AdSDPV, respectively. limits detection methods 1.47 10−8 3.96 10−9 AdSDPV.

Язык: Английский

---

Biocompatible Poly(ε-Caprolactone) Nanocapsules Enhance the Bioavailability, Antibacterial, and Immunomodulatory Activities of Curcumin

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10692 - 10692

Опубликована: Окт. 4, 2024

Curcumin (Cur), the primary curcuminoid found in Curcuma longa L., has garnered significant attention for its potential anti-inflammatory and antibacterial properties. However, hydrophobic nature significantly limits bioavailability. Additionally, adipose-derived stem cells (ADSCs) possess immunomodulatory properties, making them useful treating inflammatory autoimmune conditions. This study aims to verify efficacy of poly(ε-caprolactone) nanocapsules (NCs) improving Cur’s bioavailability, antibacterial, activities. The Cur-loaded (Cur-NCs) were characterized their physicochemical properties (particle size, polydispersity index, Zeta potential, encapsulation efficiency) stability over time. A digestion test simulated behavior Cur-NCs gastrointestinal tract. Micellar phase analyses evaluated Cur-NCs’ bioaccessibility. activity free Cur, NCs, against various Gram-positive Gram-negative strains was determined using microdilution method. ADSC viability, treated with presence or absence lipopolysaccharide, analyzed 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay. survival assessed through Muse apoptotic expression both pro-inflammatory (interleukin-1β tumor necrosis factor-α) (IL-10 transforming growth factor-β) cytokines on ADSCs by real-time polymerase chain reaction. results demonstrated high post-gastric elevated bioaccessibility Cur post-intestinal digestion. Moreover, exhibited Escherichia coli without affecting Lactobacillus growth. No changes viability observed under experimental Finally, modulated pro- exposed stimuli. Collectively, these findings highlight enhance bioavailability therapeutic efficacy, particularly cell-based treatments diseases intestinal dysbiosis.

Язык: Английский

---