Identification of key genes and immune characteristics of SASP in acute ischemic stroke DOI

Hanlu Cai,

Huixue Zhang,

Guanghao Xin

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

Abstract Background: The senescence-associated secretory phenotype (SASP) is a key mechanism through which senescent cardiovascular cells contribute to plaque formation, instability, and vascular remodeling. However, the correlation between SASP acute ischemic stroke (AIS), particularly its immune inflammation characteristics, remains underexplored requires further elucidation. Methods We downloaded AIS database from GEO obtained genes Atlas related literature. Using two machine learning algorithms, we identified five hub genes. Unsupervised cluster analysis was performed on patients with DEGs separately identify distinct gene clusters, were then analyzed for characteristics. We explored biological functions properties of by using various algorithms (GSEA, GSVA CIBERSORT). Principal component (PCA) used generate SASP-related scores based expression A logistic regression algorithm employed establish an classification diagnosis model Peripheral venous blood collected validation real-time quantitative PCR (RT-qPCR), protein assessed immunohistochemistry. Results validated them RT-qPCR. Gene revealed B differentially expressed B, indicating that samples had more severe inflammatory response higher risk disease deterioration. constructed gene-drug regulatory network PIN1and established diagnostic nomogram algorithm. Immunohistochemical thrombi PICALM PIN1. Conclusions This study expression, molecular patterns, immunological characteristics in bioinformatics methods. It provides theoretical basis research direction identifying new markers AIS, understanding thrombosis, improving classification, diagnosis, treatment, prognosis AIS.

Язык: Английский

Electroacupuncture regulates SIRT1/p53/p21 signaling pathway to prevent stress-induced premature senescence of nucleus pulposus cells in degenerative intervertebral discs DOI
Min Wang, Jiabao Huang, Jing Zou

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 148, С. 114114 - 114114

Опубликована: Янв. 31, 2025

Язык: Английский

Процитировано

1
Immunosenescence: How Aging Increases Susceptibility to Bacterial Infections and Virulence Factors DOI Creative Commons
Nikolaos Theodorakis, Georgios Feretzakis, Christos Hitas

и другие.

Microorganisms, Год журнала: 2024, Номер 12(10), С. 2052 - 2052

Опубликована: Окт. 11, 2024

The process of aging leads to a progressive decline in the immune system function, known as immunosenescence, which compromises both innate and adaptive responses. This includes impairments phagocytosis decreased production, activation, function T- B-lymphocytes, among other effects. Bacteria exploit immunosenescence by using various virulence factors evade host’s defenses, leading severe often life-threatening infections. manuscript explores complex relationship between bacterial virulence, focusing on underlying mechanisms that increase vulnerability infections elderly. Additionally, it discusses how machine learning methods can provide accurate modeling interactions weakened mechanisms, guiding development personalized interventions. vaccines, novel antibiotics, antivirulence therapies for multidrug-resistant bacteria, well investigation potential immune-boosting therapies, are promising strategies this field. Future research should focus approaches be integrated with immunological, microbiological, clinical data develop interventions improve outcomes growing elderly population.

Язык: Английский

Процитировано

6
The role of SASP in ischemic stroke: a deep dive into cellular mechanisms DOI Creative Commons

Dong Xie,

Yang Liu,

Fang-Biao Xu

и другие.

Frontiers in Neurology, Год журнала: 2025, Номер 15

Опубликована: Янв. 27, 2025

Background The escalating incidence of ischemic stroke (IS) exerts a heavy toll on global health. Aging, prominent risk factor, implicates the senescence-associated secretory phenotype (SASP) in IS pathogenesis. We postulated that alterations SASP-related factor expression during correlate with remodeling intercellular interaction networks and disease advancement. present study endeavored to preliminarily dissect SASP-IS nexus via combined bulk single-cell transcriptome analysis. Methods Aggregated profiles from human peripheral blood chips MCAO mouse sequencing data, followed by SASP gene Executed protein network enrichment assays. Investigated immune infiltration patients, managed quality control annotation cherry-picked central cells based scores, unearthed essential genes analysis, conducted pseudo-time communication studies, prognosticated drugs for hub genes. Finally, authenticated core serum Sham rats using real-time fluorescent polymerase chain reaction (RT-qPCR). Results Fourteen were discerned. Seven cell types annotated data. Basophils exhibited highest Lcp1 upregulated Ccl3 downregulated basophils group. Enrichment analysis divulged significant association apoptosis pathway responses. is pivotal basophil-neutrophil crosstalk. Additionally, we forecasted nagrestipen’s regulatory function Ccl3. RT-qPCR demonstrated marked elevation mRNA pronounced reduction group relative Conclusion its linchpin microenvironment. might potentially modulate progression influencing SASP, proffering novel prospects clinical diagnosis treatment.

Язык: Английский

Процитировано

0
The crosstalk between senescence, tumor, and immunity: molecular mechanism and therapeutic opportunities DOI Creative Commons
Zehua Wang, Chen Chen,

Jiaoyu Ai

и другие.

MedComm, Год журнала: 2025, Номер 6(1)

Опубликована: Янв. 1, 2025

Cellular senescence is characterized by a stable cell cycle arrest and hypersecretory, proinflammatory phenotype in response to various stress stimuli. Traditionally, this state has been viewed as tumor-suppressing mechanism that prevents the proliferation of damaged cells while activating immune for their clearance. However, increasingly recognized contributing factor tumor progression. This dual role necessitates careful evaluation beneficial detrimental aspects within microenvironment (TME). Specifically, senescent display unique senescence-associated secretory releases diverse array soluble factors affecting TME. Furthermore, impact on tumor-immune interaction complex often underappreciated. Senescent create an immunosuppressive TME favoring In contrast, could promote transition from evasion Given these intricate dynamics, therapies targeting hold promise advancing antitumor strategies. review aims summarize effects progression, explore its influence interactions, discuss potential therapeutic strategies, alongside challenges future directions. Understanding how regulates immunity, along with new interventions, essential managing remodeling microenvironment.

Язык: Английский

Процитировано

0
Identification of Key Genes and Immune Characteristics of SASP in Acute Ischemic Stroke DOI

Hanlu Cai,

Huixue Zhang,

Guanghao Xin

и другие.

Journal of Molecular Neuroscience, Год журнала: 2025, Номер 75(1)

Опубликована: Фев. 17, 2025

Язык: Английский

Процитировано

0
Inflammation: a matter of immune cell life and death DOI Creative Commons
Elizabeth C. Bender,

Halah S. Tareq,

Laura J. Suggs

и другие.

npj Biomedical Innovations., Год журнала: 2025, Номер 2(1)

Опубликована: Март 4, 2025

Язык: Английский

Процитировано

0
Advantages and disadvantages of targeting senescent endothelial cells in cardiovascular and cerebrovascular diseases based on small extracellular vesicles DOI
Wen Ma,

Tingyuan Zhou,

Songling Tang

и другие.

Expert Opinion on Therapeutic Targets, Год журнала: 2024, Номер unknown, С. 1 - 15

Опубликована: Окт. 30, 2024

With the growth of aging population, age-related diseases have become a heavy global burden, particularly cardiovascular and cerebrovascular (CVDs). Endothelial cell (EC) senescence constitutes an essential factor in development CVDs, prompting increased focus on strategies to alleviate or reverse EC senescence.

Язык: Английский

Процитировано

1
Identification of key genes and immune characteristics of SASP in acute ischemic stroke DOI

Hanlu Cai,

Huixue Zhang,

Guanghao Xin

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

Abstract Background: The senescence-associated secretory phenotype (SASP) is a key mechanism through which senescent cardiovascular cells contribute to plaque formation, instability, and vascular remodeling. However, the correlation between SASP acute ischemic stroke (AIS), particularly its immune inflammation characteristics, remains underexplored requires further elucidation. Methods We downloaded AIS database from GEO obtained genes Atlas related literature. Using two machine learning algorithms, we identified five hub genes. Unsupervised cluster analysis was performed on patients with DEGs separately identify distinct gene clusters, were then analyzed for characteristics. We explored biological functions properties of by using various algorithms (GSEA, GSVA CIBERSORT). Principal component (PCA) used generate SASP-related scores based expression A logistic regression algorithm employed establish an classification diagnosis model Peripheral venous blood collected validation real-time quantitative PCR (RT-qPCR), protein assessed immunohistochemistry. Results validated them RT-qPCR. Gene revealed B differentially expressed B, indicating that samples had more severe inflammatory response higher risk disease deterioration. constructed gene-drug regulatory network PIN1and established diagnostic nomogram algorithm. Immunohistochemical thrombi PICALM PIN1. Conclusions This study expression, molecular patterns, immunological characteristics in bioinformatics methods. It provides theoretical basis research direction identifying new markers AIS, understanding thrombosis, improving classification, diagnosis, treatment, prognosis AIS.

Язык: Английский

Процитировано

0