Unveiling the molecular activity of HIV towards the CD4: A study based on subtype C via docking and dynamics approach

Journal of Genetic Engineering and Biotechnology, Год журнала: 2025, Номер 23(1), С. 100457 - 100457

Опубликована: Янв. 18, 2025

Язык: Английский

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Seeking innovative concepts in development of antiviral drug combinations

Antiviral Research, Год журнала: 2025, Номер 234, С. 106079 - 106079

Опубликована: Янв. 9, 2025

Язык: Английский

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Addition of a short HIV-1 fusion-inhibitory peptide to PRO 140 antibody dramatically increases its antiviral breadth and potency

Journal of Virology, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

ABSTRACT PRO 140, a humanized anti-HIV monoclonal antibody targeting the cell coreceptor CCR5, is currently under clinical trials, but it only affects CCR5-tropic viruses. In this study, we have engineered two tandem fusion proteins (2P23-PRO140SC and 2P23-PRO140-Fc) with bifunctional activity by adding short fusion-inhibitory peptide 2P23 to single-chain fragment variable (scFv) of 140 (PRO140SC) or without Fc domain human IgG4. We first demonstrated that 2P23-PRO140SC 2P23-PRO140-Fc could efficiently bind membranes through CCR5 anchoring, which did not affect expression level on surface. then verified addition PRO140SC enabled very potent against CXCR4-tropic HIV-1 isolates. As expected, bispecific exhibited highly activities in inhibiting divergent subtypes viral mutants were resistant inhibitors T20, they displayed relatively low vitro cytotoxicity. Furthermore, both had robust vivo rats, much better than 2P23-PRO140SC. conclusion, our studies provided overcome drawbacks offered novel tools for studying mechanisms infection. IMPORTANCE Given evolves high variability drug resistance, development antivirals important. CCR5-directed inhibits The designed minimum enable dramatically increased viruses, thus offering antiviral agents functionality can antibody.

Язык: Английский

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Gut Microbiome Alteration in HIV/AIDS and the Role of Antiretroviral Therapy—A Scoping Review

Microorganisms, Год журнала: 2024, Номер 12(11), С. 2221 - 2221

Опубликована: Ноя. 1, 2024

(1) Background: The gut microbiota plays a crucial role in chronic immune activation associated with human immunodeficiency virus (HIV) infection, acquired deficiency syndrome (AIDS) pathogenesis, non-AIDS-related comorbidities, and mortality among people living HIV (PLWH). effects of antiretroviral therapy on the microbiome remain underexplored. This study aims to map evidence impact integrase strand transfer inhibitors (INSTI) non-nucleoside reverse transcriptase (NNRTI) PLWH. (2) Methods: A scoping review was conducted using PubMed, Web Science, Embase, reports collected following PRISMA for Scoping Reviews (PRISMA-ScR). (3) Results: Evidence suggests that INSTI-based regimes generally promote restoration alpha diversity, bringing it closer seronegative controls, while beta diversity remains largely unchanged. therapies are suggested be improvements composition tendency toward reduced inflammatory markers. In contrast, NNRTI-based treatments demonstrate limited recovery linked an increase proinflammatory bacteria. (4) Conclusions: Based current literature, is indicated (ART) facilitates better microbiome.

Язык: Английский

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Development of lipopeptide-based HIV-1/2 fusion inhibitors targeting the gp41 pocket site with a new design strategy

Antiviral Research, Год журнала: 2024, Номер unknown, С. 106042 - 106042

Опубликована: Ноя. 1, 2024

Язык: Английский

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