Molecular Characteristics, Heterogeneity, Plasticity, and Cell of Origin of Neuroendocrine Bladder Cancer
Опубликована: Март 6, 2025
Neuroendocrine
bladder
cancer
(NEBC)
is
a
rare
but
highly
aggressive
cancer,
representing
approximately
1%
of
urinary
cancer.
The
most
common
NEBC
small
cell
(SCBC),
characterized
by
high
rates
recurrence,
chemotherapy
resistance,
and
early
mortality.
SCBC
histologically
identical
to
lung
(SCLC)
remains
significantly
understudied.
Advances
in
next-generation
sequencing
techniques
have
partially
elucidated
the
molecular
characteristics
identified
druggable
targets.
This
review
compiles
recent
studies
on
human
samples,
summarizing
key
findings
their
genomic
alterations
subtyping.
Notably,
it
highlights
specific
mutations
TERT
promoter
epigenetic
modifiers
NEBC,
as
well
subtyping
based
lineage-specific
transcription
factors,
including
ASCL1,
NEUROD1,
POU2F3.
Furthermore,
this
explores
significant
tumor
heterogeneity
cellular
plasticity
observed
discusses
its
origin
potential
therapeutic
targets
(MET
inhibitor
or
DLL3)
preclinical
models.
Emerging
evidence
suggests
that
may
share
with
urothelial
carcinoma
(UC),
arising
from
UC
precursor.
Advancing
our
understanding
tumorigenesis
identifying
will
enhance
treatment
outcomes
for
patients
NEBC.
Язык: Английский
Modern tumor imaging models for rodents: potential and prospects in translational medicine
Bulletin of Russian State Medical University,
Год журнала:
2025,
Номер
2025(2)
Опубликована: Март 1, 2025
Rodent
neoplastic
process
models
are
extensively
used
in
pre-clinical
practice
to
assess
the
dynamics
of
tumor
development
and
test
anti-cancer
drugs,
which
ensures
flexibility
choosing
both
basic
advanced
personalized
for
researchers.
Various
modern
model
imaging
methods
considered,
including
fluorescence
bioluminescence
imaging,
enable
comprehensive
assessment,
from
qualitative
evaluation
vivo
monitoring.
We
believe
that
autonomous
bioluminescent
systems
mammalian
cells
will
provide
new
possibilities
noninvasive
animal
physiological
processes,
long-term
monitoring
progression.
Язык: Английский
Practical implementation and impact of the 4R principles in ethnopharmacology: Pursuing a more humane approach to research
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 28, 2025
Ethnopharmacology,
a
discipline
focused
on
studying
the
medicinal
use
of
natural
materials
by
humans,
plays
crucial
role
in
addressing
challenges
modern
drug
development.
However,
traditional
3R
principle-Replacement,
Reduction,
and
Refinement-have
limitations
guiding
ethical
management
animal
experimentation,
conducting
studies,
utilizing
animal-derived
ethnopharmacological
research.
To
address
these
gaps,
field
has
introduced
4R
principles,
which
expand
original
framework
adding
"Responsibility."
The
Responsibility
principle
highlights
obligation
researchers
to
consider
welfare
experimental
animals
during
all
procedures.
It
calls
for
take
accountability
their
actions
decisions,
ensuring
that
they
actively
protect
exhibit
empathy
across
species.
This
reinforces
foundation
implement
principles
effectively,
this
article
explores
dimensions
Refinement,
Replacement,
detail.
For
strategies
include
minimizing
developing
optimized,
efficient
designs,
creating
tissue
banks
recycle
samples,
improving
success
rates
modeling.
These
efforts
collectively
aim
enhance
standards
while
advancing
scientific
outcomes.
In
terms
goal
is
minimize
distress
pain
environment,
refining
operational
procedures,
strict
control
experiments
under
anesthesia,
prioritizing
non-invasive
or
minimally
invasive
techniques
data
collection.
reduce
need
exploring
alternative
solutions.
includes
substituting
vitro
vivo
ones,
using
3D
organoids
replace
organs,
applying
deep
learning
technologies
ways
decrease
use.
focuses
enhancing
researchers'
obligations
toward
welfare.
can
be
achieved
regulations
policies
governing
providing
training
technical
personnel,
promoting
awareness
practices.
introduction
implementation
provide
valuable
guidance
conduct
experimentation
research,
offering
new
insights
methodologies
support
responsible
studies.
Язык: Английский
Application of Fluorescence- and Bioluminescence-Based Biosensors in Cancer Drug Discovery
Biosensors,
Год журнала:
2024,
Номер
14(12), С. 570 - 570
Опубликована: Ноя. 24, 2024
Recent
advances
in
drug
discovery
have
established
biosensors
as
indispensable
tools,
particularly
valued
for
their
precision,
sensitivity,
and
real-time
monitoring
capabilities.
The
review
begins
with
a
brief
overview
of
cancer
discovery,
underscoring
the
pivotal
role
advancing
research.
Various
types
employed
are
then
explored,
particular
emphasis
on
fluorescence-
bioluminescence-based
technologies
such
FRET,
TR-FRET,
BRET,
NanoBRET,
NanoBiT.
These
enabled
breakthrough
discoveries,
including
identification
Celastrol
novel
YAP-TEAD
inhibitor
through
NanoBiT-based
screening,
development
TR-FRET
assays
that
successfully
identified
Ro-31-8220
SMAD4R361H/SMAD3
interaction
inducer.
integration
high
throughput
screening
validation
compounds
is
examined,
highlighting
successful
applications
LATS
revealed
VEGFR
an
upstream
regulator
Hippo
signaling
pathway.
Real-time
cellular
responses
has
yielded
invaluable
insights
into
cell
pathways,
demonstrated
by
NanoBRET
detecting
RAF
dimerization
HiBiT
systems
protein
degradation
dynamics.
addresses
challenges
linked
to
biosensor
applications,
maintaining
stability
complex
tumor
microenvironments
achieving
consistent
sensitivity
HTS
applications.
Emerging
trends
discussed,
integrating
artificial
intelligence
advanced
nanomaterials
enhanced
performance.
In
conclusion,
this
offers
comprehensive
analysis
dynamic
field,
presenting
quantitative
evidence
impact
potential
revolutionize
targeted
treatments.
Язык: Английский