In Vitro Potentiation of Doxorubicin Cytotoxicity Utilizing Clarithromycin Loaded-PEGylated Liposomes

Technology in Cancer Research & Treatment, Год журнала: 2025, Номер 24

Опубликована: Янв. 1, 2025

Doxorubicin (DOX) is a potent chemotherapeutic agent for breast cancer, but its effectiveness often diminished by resistance mechanisms, particularly through p-glycoprotein (P-gp) mediated drug efflux. Clarithromycin (CAM), macrolide antibiotic, inhibits multiple metabolic pathways including CYP3A and P-gp, potentially countering DOX resistance. This study aimed to evaluate the potentiation of against MCF-7 cancer cell line encapsulating both CAM in PEGylated liposomes. liposomes containing were prepared using thin film hydration method. The physicochemical properties liposomes, average particle size, polydispersity index (PDI), zeta potential, characterized. Encapsulation efficiencies assessed, stability was evaluated over 9 days at room temperature. Cell viability measured an IC50 assay, P-gp expression levels determined ELISA. CAM/DOX-PEGylated exhibited optimal size (238 ± 26.7 nm), PDI (0.29 0.107), potential (-20.9 2.17 mV). These maintained good regarding charge days. 81.05% 78.13% DOX. value 0.13 µM, representing significant reduction compared physical mixture (0.25 µM) free (0.21 cells. ELISA analysis showed approximately 5% with 1.61% results indicate that encapsulated enhances cells, likely inhibition p-glycoprotein. approach may offer promising strategy overcome improve chemotherapy outcomes.

Язык: Английский

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Formation and evaluation of doxorubicin and cromoglycate metal–organic framework for anti-cancer activity

Nanomedicine, Год журнала: 2025, Номер unknown, С. 1 - 13

Опубликована: Янв. 31, 2025

We develop and evaluate copper-based metal-organic frameworks (Cu-MOFs) incorporating cromolyn as a linker to enhance structural stability, drug delivery efficiency, therapeutic potential, particularly for breast cancer treatment. Two Cu-MOF formulations were synthesized: Cu-MOFs-BDC-DOX (using terephthalic acid) Cu-MOFs-CROMO-DOX linker). Characterization was performed using SEM/TEM morphology, FTIR, XRD, TGA confirm integrity. Drug encapsulation efficiency release profiles assessed, followed by in vitro cytotoxicity, cell migration, colony formation assays MDA-MB-231 cells. Both demonstrated high (83-91%) sustained over 48 h at pH 7.4. exhibited superior cytotoxicity with an IC50 of 0.88 ± 0.07 µM compared 7.1 0.11 Cu-MOFs-BDC-DOX. inhibit migration dose-dependent manner. The formulation enhanced outperforming its counterpart targeting This study highlights the promise MOF-based nanocarriers overcoming limitations conventional chemotherapy, offering pathway more effective targeted treatments reduced side effects.

Язык: Английский

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Withania somnifera Ameliorates Doxorubicin-Induced Nephrotoxicity and Potentiates Its Therapeutic Efficacy Targeting SIRT1/Nrf2, Oxidative Stress, Inflammation, and Apoptosis

Pharmaceuticals, Год журнала: 2025, Номер 18(2), С. 248 - 248

Опубликована: Фев. 12, 2025

Background: Doxorubicin (DOX) is a very powerful chemotherapy drug. However, its severe toxicity and potential for resistance development limit application. Withania somnifera L. Dunal (WIT) has therapeutic capacities, including anti-inflammatory, antioxidant, anticancer activities. This study investigates the preventative benefits of standardized WIT extract against DOX-induced renal damage in vivo. We also investigate synergistic effects combining DOX to improve efficacy breast cancer cells (MCF7-ADR). Methods: employed an animal model where rats were administered 300 mg/kg/day orally duration 14 days. Rats received injections at dose 5 mg/kg, total 15 mg, on 6th, 8th, 10th Results: Present results revealed that reduced increase levels blood urea creatinine activity kidney injury molecule-1. tissue damage, oxidative stress, pro-inflammatory markers. alleviated nuclear factor erythroid 2-related 2, heme oxygenase-1, sirtuin 1 tissues. modulated factor-κB decreased apoptotic indicators. Furthermore, improves DOX's capacity kill drug-resistant MCF7-ADR by arresting cell cycle promoting apoptosis. Chemical analysis root 34 distinct compounds, alkaloids, withanolides, flavanones, fatty acids. Conclusions: These constituents synergistically contribute WIT's anti-apoptotic properties. In addition, they confirm ability reduce systemic while improving treatment efficacy.

Язык: Английский

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How Advanced are Self-Assembled Nanomaterials for Targeted Drug Delivery? A Comprehensive Review of the Literature

International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 2133 - 2161

Опубликована: Фев. 1, 2025

The development of effective drug delivery systems is a key focus in pharmaceutical research, aiming to enhance therapeutic efficacy while minimizing adverse effects. Self-assembled nanostructures present promising solution due their tunable properties, biocompatibility, and ability encapsulate deliver agents specific targets. This review examines recent advancements drug-based self-assembled for targeted applications, including drug-drug conjugates, polymeric-based architectures, biomolecules, peptides, DNA, squalene conjugates amphiphilic drugs. Various strategies fabricating these are discussed, with an emphasis on the design principles mechanisms underlying self-assembly potential tissues or cells. Furthermore, integration targeting ligands, stimuli-responsive moieties imaging into explored enhanced outcomes real-time monitoring. Challenges such as stability, scalability regulatory hurdles translating from bench bedside also addressed. Drug-based represent platform developing next-generation improved reduced side

Язык: Английский

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Neuroprotective Effects of Granulocyte Colony-Stimulating Factor Against Tramadol-Induced Cerebellar Neurotoxicity

Tissue and Cell, Год журнала: 2025, Номер 94, С. 102832 - 102832

Опубликована: Март 1, 2025

Язык: Английский

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