Podophyllic Aldehyde, a Podophyllotoxin Derivate, Elicits Different Cell Cycle Profiles Depending on the Tumor Cell Line: A Systematic Proteomic Analysis DOI Open Access
Ángela‐Patricia Hernández, Lorea Chaparro‐González,

Olga Garzo-Sánchez

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(9), С. 4631 - 4631

Опубликована: Апрель 24, 2024

When new antitumor therapy drugs are discovered, it is essential to address target molecules from the point of view chemical structure and carry out efficient systematic evaluation. In case natural products derived compounds, special importance investigate chemomodulation further explore antitumoral pharmacological activities. this work, compound podophyllic aldehyde, a cyclolignan product podophyllotoxin, has been evaluated for its viability, influence on cell cycle, effects intracellular signaling. We used functional proteomics characterization Compared with FDA-approved drug etoposide (another podophyllotoxin derivative), we found interesting results regarding cytotoxicity aldehyde. addition, were able observe effect mitotic arrest in treated cells. The use aldehyde resulted increased solid tumor lines, compared etoposide, blocked cycle more successfully than etoposide. High-throughput analysis deregulated proteins revealed selective antimitotic mechanism action HT-29 line, contrast other hematological lines. Also, apoptotic profile was deciphered. death activated independently profile. these targeted analyses have also shown significant response signaling kinases, key involved cascades proliferation or metastasis. Thanks comprehensive remarkable cytotoxic, antimitotic, features discovered that will repurpose transformations analysis.

Язык: Английский

Podophyllotoxin: Recent Advances in the Development of Hybridization Strategies to Enhance Its Antitumoral Profile DOI Creative Commons
Carolina Miranda‐Vera, Ángela‐Patricia Hernández, Pilar Garcı́a Garcı́a

и другие.

Pharmaceutics, Год журнала: 2023, Номер 15(12), С. 2728 - 2728

Опубликована: Дек. 4, 2023

Podophyllotoxin is a naturally occurring cyclolignan isolated from rhizomes of Podophyllum sp. In the clinic, it used mainly as an antiviral; however, its antitumor activity even more interesting. While podophyllotoxin possesses severe side effects that limit development anticancer agent, nevertheless, has become good lead compound for synthesis derivatives with fewer and better selectivity. Several examples, such etoposide, highlight potential this natural product chemomodulation in search new agents. This review focuses on recent chemical modifications (2017-mid-2023) skeleton performed at C-ring (but also lactone D-ring trimethoxyphenyl E-ring) together their biological properties. Special emphasis placed hybrids or conjugates other products (either primary secondary metabolites) molecules (heterocycles, benzoheterocycles, synthetic drugs, moieties) contribute to improved bioactivity. fact, hybridization been strategy design enhanced The way which two components are joined (directly through spacers) was considered organization review. comprehensive perspective presented aim guiding medicinal chemistry community podophyllotoxin-based drugs

Язык: Английский

Процитировано

9

Insights into podophyllotoxin lactone features: New cyclolignans as potential dual tubulin‐topoisomerase II inhibitors DOI Creative Commons
Ángela‐Patricia Hernández,

Celia Rosales‐Fernández,

Carolina Miranda‐Vera

и другие.

Archiv der Pharmazie, Год журнала: 2024, Номер 358(1)

Опубликована: Ноя. 12, 2024

Abstract Chemomodulation of natural cyclolignans as podophyllotoxin has been a successful approach to obtain semisynthetic bioactive derivates. One example this is the FDA‐approved drug etoposide for solid and hematological tumors. It differs from antimitotic activity product in its mechanism action, being topoisomerase II inhibitor instead tubulin antimitotic. Within molecular requirements these compounds, trans‐γ‐lactone moiety presented parent compound always feature be explored chemomodulate bioactivity. In study, we have obtained different compounds that comply with characteristics antitubulin antitopoisomerase combined single molecule. Furthermore, influence trans ‐lactone on final activity, finding cis was also interesting terms The best values cytotoxicity cell cycle inhibition were lacking lactone ring, thus mimicking podophyllic aldehyde functionalization, selective derivate. analogs cytotoxic activity. present study illustrates potential chemomodulation products such cyclolignan derivates discovery new antitumor agents.

Язык: Английский

Процитировано

1

Cytotoxic Cyclolignans Obtained by the Enlargement of the Cyclolignan Skeleton of Podophyllic Aldehyde, a Selective Podophyllotoxin-Derived Cyclolignan DOI Creative Commons
Pablo A. García, Ángela‐Patricia Hernández,

Ma Antonia Gómez-Zurita

и другие.

Molecules, Год журнала: 2024, Номер 29(7), С. 1442 - 1442

Опубликована: Март 23, 2024

Podophyllotoxin, a cyclolignan natural product, has been the object of extensive chemomodulation to obtain better chemotherapeutic agents. Among obtained podophyllotoxin derivatives, podophyllic aldehyde showed very interesting potency and selectivity against several tumoral cell lines, so it became our lead compound for further modifications, as described in this work, oriented toward enlargement skeleton. Thus, modifications performed at function included nucleophilic addition reactions incorporation carbon into five-membered rings, such thiazolidinones benzo-fused azoles. The synthesized derivatives were evaluated types cancer cells, although some compounds cytotoxic nanomolar range, most them less potent selective than parent aldehyde, with being those having lactone ring podophyllotoxin. In silico ADME evaluation predicted good druggability them. results indicate that γ-lactone is important potency, while α,β-unsaturated necessary induce these cyclolignans.

Язык: Английский

Процитировано

0

Podophyllic Aldehyde, a Podophyllotoxin Derivate, Elicits Different Cell Cycle Profiles Depending on the Tumor Cell Line: A Systematic Proteomic Analysis DOI Open Access
Ángela‐Patricia Hernández, Lorea Chaparro‐González,

Olga Garzo-Sánchez

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(9), С. 4631 - 4631

Опубликована: Апрель 24, 2024

When new antitumor therapy drugs are discovered, it is essential to address target molecules from the point of view chemical structure and carry out efficient systematic evaluation. In case natural products derived compounds, special importance investigate chemomodulation further explore antitumoral pharmacological activities. this work, compound podophyllic aldehyde, a cyclolignan product podophyllotoxin, has been evaluated for its viability, influence on cell cycle, effects intracellular signaling. We used functional proteomics characterization Compared with FDA-approved drug etoposide (another podophyllotoxin derivative), we found interesting results regarding cytotoxicity aldehyde. addition, were able observe effect mitotic arrest in treated cells. The use aldehyde resulted increased solid tumor lines, compared etoposide, blocked cycle more successfully than etoposide. High-throughput analysis deregulated proteins revealed selective antimitotic mechanism action HT-29 line, contrast other hematological lines. Also, apoptotic profile was deciphered. death activated independently profile. these targeted analyses have also shown significant response signaling kinases, key involved cascades proliferation or metastasis. Thanks comprehensive remarkable cytotoxic, antimitotic, features discovered that will repurpose transformations analysis.

Язык: Английский

Процитировано

0