Targeted Glioblastoma Treatment via Synthesis and Functionalization of Gold Nanoparticles With De Novo–Engineered Transferrin-Like Peptides: Protocol for a Novel Method (Preprint) DOI
Augusto Müller Fiedler, Michelle Medeiros, Haidi D. Fiedler

и другие.

Опубликована: Май 28, 2023

BACKGROUND Glioblastoma multiforme (GBM) is an aggressive brain tumor with limited treatment options due to the blood-brain barrier’s (BBB’s) impedance and inherent resistance chemotherapy. Gold nanoparticles (AuNPs) functionalized transferrin-like peptides show promise in overcoming these challenges, enhancing drug delivery brain, reducing chemotherapy resistance. OBJECTIVE The primary goal of this study establish a detailed protocol for synthesizing stabilizing AuNPs, functionalizing them de novo–engineered peptides, conjugating chemotherapeutic agent temozolomide. This strategy aims improve across BBB circumvent secondary objective includes assessment safety potential vivo use synthesized nanoparticle complex. METHODS proposal involves multiple steps rigorous quality control AuNP synthesis, stabilization surfactants, polyethylene glycol coating. engineered will be attached AuNPs’ surface, followed by attachment temozolomide O6-methylguanine-DNA methyltransferase inhibitors. resulting complex undergo vitro testing assess penetration, efficacy against GBM cells, toxicity. RESULTS Initial preliminary experiments simulations suggest successful synthesis AuNPs effective peptides. We propose peptide verification using Fourier transform infrared spectroscopy surface plasmon resonance. Additionally, we conduct pH stability tests ensure our retain their properties acidic microenvironments. CONCLUSIONS proposed functionalization represents novel approach treatment. Our opens new avenues reduction. While primarily focus on studies computational modeling at stage, completion lead further development, including design optimization. anticipates inspiring future research funding neuro-oncology, presenting potentially innovative option GBM. INTERNATIONAL REGISTERED REPORT RR1-10.2196/49417

Язык: Английский

A Step Forward for the Treatment of Localized Prostate Cancer Using Gold Nanoparticles Combined with Laser Irradiation DOI Open Access
Sara Pinho, Tânia Ferreira-Gonçalves, Joana Lopes

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(8), С. 4488 - 4488

Опубликована: Апрель 19, 2024

Prostate cancer (PCA) is the second most common diagnosis in men and fifth leading cause of death worldwide. The conventional treatments available are beneficial to only a few patients and, those, some present adverse side effects that eventually affect quality life patients. Thus, there an urgent need for effective, less invasive targeted specific PCA. Photothermal therapy (PTT) minimally provides localized effect tumour cell ablation by activating photothermal agents (PTA) mediate conversion light beam’s energy into heat at site. As tumours unable easily dissipate heat, they become more susceptible temperature increases. In PTT field, gold nanoparticles (AuNPs) have been attracting interest as PTA. aim this study was formulate AuNPs capable remaining retained subsequently generating were synthesized characterized terms size, polydispersity index (PdI), zeta potential (ZP), morphology surface plasmon resonance (SPR). safety their efficacy assessed using vitro models. A preliminary vivo assessment with mean size lower than 200 nm confirmed. spherical-like SPR band showed good absorbance laser wavelength. Without laser, proved be safe both (>70% viability) vivo. addition, irradiation, relatively effective PCA cells. Overall, formulation appears promising use PTT.

Язык: Английский

Процитировано

7
A review on toxicity mechanism and risk factors of nanoparticles in respiratory tract DOI
Vidyasri Khadanga, Purna Chandra Mishra

Toxicology, Год журнала: 2024, Номер 504, С. 153781 - 153781

Опубликована: Март 15, 2024

Язык: Английский

Процитировано

6
Advances in localized prostate cancer: a special focus on photothermal therapy DOI Creative Commons
Sara Pinho, João M. P. Coelho, Maria Manuela Gaspar

и другие.

European Journal of Pharmacology, Год журнала: 2024, Номер unknown, С. 176982 - 176982

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

5
Gold nanoparticles and their applications in transdermal drug delivery: A review DOI

Kanokwan Singpanna,

Chaiyakarn Pornpitchanarong, Prasopchai Patrojanasophon

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2023, Номер 90, С. 105174 - 105174

Опубликована: Ноя. 17, 2023

Язык: Английский

Процитировано

12
ISCOM-type matrix from beta-escin and glycyrrhizin saponins DOI Creative Commons
V. Petkov, Sonya Tsibranska, Iliyan Manoylov

и другие.

Heliyon, Год журнала: 2025, Номер 11(2), С. e41935 - e41935

Опубликована: Янв. 1, 2025

Nanotechnology provides the opportunity for construction of modern transport devices such as nanoparticles a variety applications in field medicine. A novel experimental protocol formation saponin-cholesterol-phospholipid vesicular structure has been developed and applied to prepare stable using escin or glycyrrhizin saponins. The methods nanoparticle include sonication at 90 °C initial mixture components, followed by an additional on next day incorporation amount cholesterol, thus forming unilamellar vesicles. Tests assays cell viability, erythrocyte hemolysis, flow cytometry, fluorescent microscopy analyses have performed. By selecting appropriate component ratios, safe particles were formulated with respect tested bio-cells. prepared mean diameter between 70 130 nm, depending their composition. versatility these allows encapsulation various molecules, either within vesicle interior water-soluble components walls hydrophobic components. saponin formed after cholesterol post-addition (E3-M2) are 100 % cells remain viable even 10-times dilution particle suspension. These successful included into isolated mouse macrophages. Among generated nanoparticles, E3-M2 demonstrated properties efficient future vaccine design antigen targeting immune system.

Язык: Английский

Процитировано

0
Recent Advances in Monodisperse Gold Nanoparticle Delivery, Synthesis, and Emerging Applications in Cancer Therapy DOI

Shamim Shamim,

Shahzad Ali, Tarmeen Ali

и другие.

Plasmonics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Язык: Английский

Процитировано

0
Radioactive gold nanoparticles coated with BSA: A promising approach for prostate cancer treatment DOI Creative Commons
Angélica Bueno Barbezan, Wilmmer Alexander Arcos Rosero,

Daniel Vieira Perez

и другие.

Nanotheranostics, Год журнала: 2023, Номер 8(1), С. 112 - 126

Опубликована: Дек. 26, 2023

Nanotechnology has revolutionized medicine, especially in oncological treatments. Gold nanoparticles (AuNPs) stand out as an innovative alternative due to their biocompatibility, potential for surface modification, and effectiveness radiotherapeutic techniques. Given that prostate cancer ranks one of the leading malignancies among men, there's a pressing need investigate new therapeutic approaches.

Язык: Английский

Процитировано

7
Enhanced Cytotoxicity against a Pancreatic Cancer Cell Line Combining Radiation and Gold Nanoparticles DOI Creative Commons
Alexandra Martins, Brígida Ferreira, Maria Manuela Gaspar

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(7), С. 900 - 900

Опубликована: Июль 5, 2024

The present work consisted of an exploratory study aiming to evaluate in vitro the potential AuNPs during Radiation Therapy (RT) human pancreatic adenocarcinoma cells. coated with hyaluronic and oleic acids (HAOA-AuNPs) or bombesin peptides (BBN-AuNPs) were used. characterized by Atomic Force Microscopy (AFM) Dynamic Light Scattering. BxPC-3 tumor cells irradiated a 6 MV X-rays beam, absence presence AuNPs. AFM showed that HAOA-AuNPs BBN-AuNPs are spherical mean size 83 ± 20 nm 49 12 nm, respectively. For RT alone, reduction cell viability up 33 12% was obtained compared control (p ≤ 0.0001). alone at 200 400 μM 4% 35 4%, respectively, while for BBN-AuNPs, 50 μM, 25 3% 37 obtained, < At 72 h post-irradiation, decrease 26 22 2% between + 0.004). combination led significant control, thus representing improved effect.

Язык: Английский

Процитировано

2
Gold Nanoparticles for Photothermal Therapy – influence of experimental conditions on the properties of resulting AuNPs DOI Creative Commons
Mariana Amaral, Daniela Nunes, Elvira Fortunato

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер unknown, С. 106215 - 106215

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

2
Green synthesis of Brassica carinata microgreen silver nanoparticles, characterization, safety assessment, and antimicrobial activities DOI Creative Commons

Dogfounianalo Somda,

Joel L. Bargul, John Wesonga

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 26, 2024

Nanotechnology has been a central focus of scientific investigation over the past decades owing to its versatile applications. The synthesis silver nanoparticles (AgNPs) through plant secondary metabolites is cost-effective and eco-friendly approach. present study employed Brassica carinata microgreen extracts (BCME) promote reduction nitrate (AgNO

Язык: Английский

Процитировано

2