Biology,
Год журнала:
2023,
Номер
12(7), С. 976 - 976
Опубликована: Июль 10, 2023
The
immune
system
plays
an
important
role
in
the
skeletal
during
bone
repair
and
regeneration.
controlled
release
of
biological
factors
from
could
facilitate
optimize
remodeling
process
through
regulation
activities
cells.
This
study
aimed
to
determine
effect
delivery
immunomodulatory
biologicals
on
Immunostimulatory
cytosine-phosphate-guanosine
oligodeoxynucleotides
(CpG
ODN)
glucosylxanthone
Mangiferin
(MAG)-embedded
microbeads
were
incubated
with
P.
gingivalis-challenged
splenocytes,
or
co-cultured
RAW264.7
CpG
ODN/MAG-containing
regeneration
was
then
tested
vivo
a
mouse
alveolar
defect
model.
results
demonstrated
that
MAG
significantly
antagonized
gingivalis
proliferation
reduced
live/dead
cell
ratio.
After
addition
ODN
+
microbeads,
anti-inflammatory
cytokines
IL-10
IL-4
upregulated
day
2
but
not
4,
whereas
pro-inflammatory
cytokine
IL-1β
responses
showed
no
difference
at
both
timepoints.
RANKL
production
by
splenocytes
TRAP+
formation
cells
inhibited
microbeads.
Alveolar
bony
defects,
filled
increased
new
after
4
weeks.
In
summary,
this
evaluated
hydrogel-based
regimen
for
local
regenerate
defects.
combined
treatment
may
promote
anti-microbial/anti-inflammatory
effects
inhibition
RANKL-mediated
osteoclastogenesis.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(30)
Опубликована: Авг. 17, 2024
Abstract
Nucleic
acid
drugs
are
one
of
the
hot
spots
in
field
biomedicine
recent
years,
and
play
a
crucial
role
treatment
many
diseases.
However,
its
low
stability
difficulty
target
drug
delivery
bottlenecks
restricting
application.
Hydrogels
proven
to
be
promising
for
improving
nucleic
drugs,
reducing
adverse
effects
rapid
degradation,
sudden
release,
unnecessary
diffusion
drugs.
In
this
review,
strategies
loading
hydrogels
summarized
various
biomedical
research,
classify
mechanism
principles
these
strategies,
including
electrostatic
binding,
hydrogen
bond
based
hydrophobic
covalent
binding
indirect
using
carriers.
addition,
review
also
describes
release
photostimulation‐based
enzyme‐responsive
pH‐responsive
temperature‐responsive
release.
Finally,
applications
future
research
directions
delivering
medicine
discussed.
Chemical Engineering Journal,
Год журнала:
2024,
Номер
493, С. 152762 - 152762
Опубликована: Июнь 1, 2024
In
this
study,
an
injectable,
porous,
and
Mg2+-loaded
hydrogel
with
the
ability
to
scavenge
local
reactive
oxygen
species
was
developed
for
bone-repair
applications.
Alendronate
sodium
a
specific
binding
affinity
Mg2+
incorporated
into
through
Schiff's
base
reaction
oxidized
dextran.
The
porous
hydrogel,
which
cross-linked
by
catechol-modified
chitosan
maintain
its
mechanical
properties,
showed
enhanced
cell
viability,
proliferation,
differentiation
of
human
bone
marrow
stromal
cells
in
vitro,
osteogenic
property
rat-femur
defect
model.
promoted
regeneration
tissue
including
ossification
particularly
endochondral
ossification,
indicating
progressive
healing
process.
formed
exhibited
dense
layered
structure,
substantiating
effectiveness
differentiation.
also
affected
polarization
macrophages
inhibited
M1
phenotype
associated
repair
regeneration,
exhibiting
potential
reduce
inflammatory
response
benefit
repair.
Overall,
can
be
anticipated
as
promising
biomaterial
because
multiple
biofunctions
osteogenesis
modulation
inflammation.
Biology,
Год журнала:
2023,
Номер
12(7), С. 976 - 976
Опубликована: Июль 10, 2023
The
immune
system
plays
an
important
role
in
the
skeletal
during
bone
repair
and
regeneration.
controlled
release
of
biological
factors
from
could
facilitate
optimize
remodeling
process
through
regulation
activities
cells.
This
study
aimed
to
determine
effect
delivery
immunomodulatory
biologicals
on
Immunostimulatory
cytosine-phosphate-guanosine
oligodeoxynucleotides
(CpG
ODN)
glucosylxanthone
Mangiferin
(MAG)-embedded
microbeads
were
incubated
with
P.
gingivalis-challenged
splenocytes,
or
co-cultured
RAW264.7
CpG
ODN/MAG-containing
regeneration
was
then
tested
vivo
a
mouse
alveolar
defect
model.
results
demonstrated
that
MAG
significantly
antagonized
gingivalis
proliferation
reduced
live/dead
cell
ratio.
After
addition
ODN
+
microbeads,
anti-inflammatory
cytokines
IL-10
IL-4
upregulated
day
2
but
not
4,
whereas
pro-inflammatory
cytokine
IL-1β
responses
showed
no
difference
at
both
timepoints.
RANKL
production
by
splenocytes
TRAP+
formation
cells
inhibited
microbeads.
Alveolar
bony
defects,
filled
increased
new
after
4
weeks.
In
summary,
this
evaluated
hydrogel-based
regimen
for
local
regenerate
defects.
combined
treatment
may
promote
anti-microbial/anti-inflammatory
effects
inhibition
RANKL-mediated
osteoclastogenesis.
