Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration DOI
Wei-Cheng Chen, Dachuan Liu, Kai Lü

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 28, 2024

Abstract Musculoskeletal diseases have emerged as the leading cause of disability worldwide, with their prevalence increasing annually. In light this escalating health challenge, organoids, an emerging technology in tissue engineering, offer promising solutions for disease modeling, drug screening, regeneration, and repair processes. The successful development musculoskeletal organoids represents a significant breakthrough, providing novel platform studying facilitating discovery new treatments. Moreover, serve valuable complements to traditional 2D culture methods animal models, offering rich insights into biology. This review provides overview organoid technology, outlining construction processes various highlighting similarities differences. Furthermore, challenges associated systems are discussed future perspectives offered.

Язык: Английский

Ginkgo biloba: A Leaf of Hope in the Fight against Alzheimer’s Dementia: Clinical Trial Systematic Review DOI Creative Commons

Guilherme Lopes de Oliveira Pagotto,

Livia Maria Oliveira dos Santos,

Najwa Osman

и другие.

Antioxidants, Год журнала: 2024, Номер 13(6), С. 651 - 651

Опубликована: Май 27, 2024

Alzheimer’s disease (AD) is a stealthy and progressive neurological disorder that leading cause of dementia in the global elderly population, imposing significant burden on both society. Currently, condition treated with medications alleviate symptoms. Nonetheless, these drugs may not consistently produce desired results can serious side effects. Hence, there vigorous pursuit alternative options to enhance quality life for patients. Ginkgo biloba (GB), an herb historical use traditional medicine, contains bioactive compounds such as terpenoids (Ginkgolides A, B, C), polyphenols, organic acids, flavonoids (quercetin, kaempferol, isorhamnetin). These are associated anti-inflammatory, antioxidant, neuroprotective properties, making them valuable cognitive health. A systematic search across three databases using specific keywords—GB AD dementia—yielded 1702 documents, selection 15 clinical trials synthesis. In eleven studies, GB extract/EGb 761® was shown improve function, neuropsychiatric symptoms, functional abilities types. four however, were no differences between GB-treated placebo groups. Significant improvements observed scores obtained from Mini-Mental State Examination (MMSE), Short Cognitive Performance Test (SKT), Neuropsychiatric Inventory (NPI). While majority synthesized show has promising potential treatment conditions, more research needed determine optimal dosages, effective delivery methods, appropriate pharmaceutical formulations. Furthermore, thorough assessment adverse effects, exploration long-term implications, investigation into drug interactions critical aspects must be carefully evaluated future studies.

Язык: Английский

Процитировано

10

Limitations of human brain organoids to study neurodegenerative diseases: a manual to survive DOI Creative Commons

Nerea Urrestizala-Arenaza,

Sonia Cerchio,

Fabio Cavaliere

и другие.

Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 18

Опубликована: Июль 9, 2024

In 2013, M. Lancaster described the first protocol to obtain human brain organoids. These organoids, usually generated from human-induced pluripotent stem cells, can mimic three-dimensional structure of brain. While they recapitulate salient developmental stages brain, their use investigate onset and mechanisms neurodegenerative diseases still faces crucial limitations. this review, we aim highlight these limitations, which hinder organoids becoming reliable models study such as Alzheimer’s disease (AD), Parkinson’s (PD), amyotrophic lateral sclerosis (ALS). Specifically, will describe structural biological impediments, including lack an aging footprint, angiogenesis, myelination, inclusion functional immunocompetent microglia—all important factors in neurodegeneration AD, PD, ALS. Additionally, discuss technical limitations for monitoring microanatomy electrophysiology parallel, propose solutions overcome current thereby making a more tool model neurodegeneration.

Язык: Английский

Процитировано

7

Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders DOI Creative Commons
Ziqin Yang,

Nicole A. Teaney,

Elizabeth D. Buttermore

и другие.

Frontiers in Neuroscience, Год журнала: 2025, Номер 18

Опубликована: Янв. 8, 2025

Neurodevelopmental disorders (NDDs) affect 4.7% of the global population and are associated with delays in brain development a spectrum impairments that can lead to lifelong disability even mortality. Identification biomarkers for accurate diagnosis medications effective treatment lacking, part due historical use preclinical model systems do not translate well clinic neurological disorders, such as rodents heterologous cell lines. Human-induced pluripotent stem cells (hiPSCs) promising vitro system modeling NDDs, providing opportunities understand mechanisms driving NDDs human neurons. Functional assays, including patch clamping, multielectrode array, imaging-based popular tools employed hiPSC disease models investigation. Recent progress machine learning (ML) algorithms also presents unprecedented advance NDD research process. In this review, we compare two-dimensional three-dimensional formats modeling, discuss applications functional offer insights on incorporating ML into hiPSC-based drug screening.

Язык: Английский

Процитировано

0

Guidelines for Manufacturing and Application of Organoids: Brain DOI Open Access
Taehwan Kwak, Si-Hyung Park,

Siyoung Lee

и другие.

International Journal of Stem Cells, Год журнала: 2024, Номер 17(2), С. 158 - 181

Опубликована: Май 23, 2024

Taehwan Kwak, Si-Hyung Park, Siyoung Lee, Yujeong Shin, Ki-Jun Yoon, Seung-Woo Cho, Jong-Chan Seung-Ho Yang, Heeyeong Heh-In Im, Sun-Ju Ahn, Woong Sun, Ji Hun Yang. IJSC -0001;0:. https://doi.org/10.15283/ijsc24056

Процитировано

3

Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration DOI
Wei-Cheng Chen, Dachuan Liu, Kai Lü

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 28, 2024

Abstract Musculoskeletal diseases have emerged as the leading cause of disability worldwide, with their prevalence increasing annually. In light this escalating health challenge, organoids, an emerging technology in tissue engineering, offer promising solutions for disease modeling, drug screening, regeneration, and repair processes. The successful development musculoskeletal organoids represents a significant breakthrough, providing novel platform studying facilitating discovery new treatments. Moreover, serve valuable complements to traditional 2D culture methods animal models, offering rich insights into biology. This review provides overview organoid technology, outlining construction processes various highlighting similarities differences. Furthermore, challenges associated systems are discussed future perspectives offered.

Язык: Английский

Процитировано

0