Preparation and Characterization of Tiamulin-Loaded Niosomes for Oral Bioavailability Enhancement in Mycoplasma-Infected Broilers DOI Creative Commons

Shimaa G. Abonashey,

Amr Gamal, Hatem A. F. M. Hassan

и другие.

Micro, Год журнала: 2024, Номер 4(4), С. 734 - 750

Опубликована: Ноя. 27, 2024

Mycoplasma infections pose significant challenges in the poultry industry, necessitating effective therapeutic interventions. Tiamulin, a veterinary antibiotic, has demonstrated efficacy against species. However, emergence of resistant species could dramatically reduce potential, contributing to economic losses. Optimizing tiamulin’s pharmacokinetic profile via nanocarrier incorporation enhance its potential and administration frequency, ultimately reducing strain emergence. Niosomes, type self-assembled non-ionic surfactant-based nanocarrier, have emerged as promising drug delivery system, offering improved stability, sustained release, enhanced bioavailability. In this study, niosomal nanocarriers encapsulating tiamulin were prepared, characterized assessed Mycoplasma-inoculated broilers following oral administration. Differential scanning colorimetry (DSC) confirmed alterations crystalline state components integration into structures formed during formulation procedure. Transmission electron microscopy (TEM) showed spherical nanostructure niosomes. The formulated exhibited zeta average hydrodynamic diameter −10.65 ± 1.37 mV 339.67 30.88 nm, respectively. Assessment parameters gallisepticum-infected revealed ability increase bioavailability systemic exposure, marked by significantly higher area under curve (AUC) (p < 0.01) prolonged elimination half-life (T1/2) 0.05). Enhanced residence time are crucial factors maintaining concentrations at reduced doses frequencies. This approach provides viable strategy decrease risk subtherapeutic levels, thereby mitigating development antibiotic resistance. findings presented herein offer sustainable for efficient use antibiotics medicine.

Язык: Английский

Deploying nucleic acids-loaded plant-derived exosomes as green nano gadget in cancer gene therapy DOI Creative Commons

Marola Paula Fawzy,

Hatem A. F. M. Hassan, Muhammad Umair Amin

и другие.

Materials Advances, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The pursuit of effective drug delivery systems is critical in advancing cancer therapies, particularly the realms chemotherapy, radiotherapy and immunotherapy.

Язык: Английский

Процитировано

0
Lathyrol inhibits the proliferation of renca cells by affecting the expression of TGF-β/smad pathway and then affecting the cell cycle DOI Creative Commons

Shengyou Song,

Yalin Song

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Abstract Background Renal cell carcinoma (RCC) is a prevalent malignant tumor with high morbidity and mortality. The TGF-β/smad signaling pathway plays significant role in the development progression of RCC. Methods This study explored impact lathyrol on proliferation mice RCC renca cells by inhibiting arresting cycle. Bioinformatics analysis, culture experiments, animal experiments were conducted to detect effects activity, mRNA, protein expressions xenograft tumors, as well cycle proteins regulatory proteins. Results Lathyrol treatment showed positive correlation inhibitory effect proliferation. IC values 786-O comparable. In vitro, decreased mRNA TGF-β1, TGF-βR1, smad2, smad3, smad4, smad9, while increasing smad4. vivo, suppressed smad9 xenografts, cyclinD1, cyclinB1, cyclinA1, cyclinE1, CDK6, CDK4, CDK1, P16, P21, P27. Conclusion can repress expression key pathway, impede signal transduction, arrest cells, subsequently inhibit cells. Future studies are needed further explore mechanism treatment.

Язык: Английский

Процитировано

0
Sustainable Nanomedicine: Enhancement of Asplatin’s Cytotoxicity In Vitro and In Vivo Using Green-Synthesized Zinc Oxide Nanoparticles Formed via Microwave-Assisted and Gambogic Acid-Mediated Processes DOI Creative Commons
Hatem A. F. M. Hassan, Nada K. Sedky, Mohamed S. Nafie

и другие.

Molecules, Год журнала: 2024, Номер 29(22), С. 5327 - 5327

Опубликована: Ноя. 12, 2024

Chemoresistance encountered using conventional chemotherapy demands novel treatment approaches. Asplatin (Asp), a platinum (IV) prodrug designed to release cisplatin and aspirin in reductive environment, has demonstrated high cytotoxicity at reduced drug resistance. Herein, we investigated the ability of green-synthesized nanocarriers enhance Asp’s efficacy. Zinc oxide nanoparticles (ZnO-NPs) were synthesized green microwave-assisted method with reducing capping agent gambogic acid (GA). These then loaded Asp, yielding Asp@ZnO-NPs. Transmission electron microscopy was utilized study morphological features ZnO-NPs. Cell viability studies conducted on MDA-MB-231 breast cancer cells Asp@ZnO-NPs significantly decrease half-maximal inhibitory concentration (IC50) (5 ± 1 µg/mL). This further flow cytometric analysis that revealed capacity increase late apoptotic fractions. Furthermore, vivo carried out solid Ehrlich carcinoma-bearing mice showed significant tumor volume reduction (156.3 7.6 mm3), compared Asp alone (202.3 8.4 mm3) untreated controls (342.6 10.3 mm3). The histopathological increased necrosis Asp@ZnO-NPs-treated group. Asp@ZnO-NPs, an eco-friendly approach, enhanced anticancer activity, offering sustainable solution for potent formulations.

Язык: Английский

Процитировано

1
Preparation and Characterization of Tiamulin-Loaded Niosomes for Oral Bioavailability Enhancement in Mycoplasma-Infected Broilers DOI Creative Commons

Shimaa G. Abonashey,

Amr Gamal, Hatem A. F. M. Hassan

и другие.

Micro, Год журнала: 2024, Номер 4(4), С. 734 - 750

Опубликована: Ноя. 27, 2024

Mycoplasma infections pose significant challenges in the poultry industry, necessitating effective therapeutic interventions. Tiamulin, a veterinary antibiotic, has demonstrated efficacy against species. However, emergence of resistant species could dramatically reduce potential, contributing to economic losses. Optimizing tiamulin’s pharmacokinetic profile via nanocarrier incorporation enhance its potential and administration frequency, ultimately reducing strain emergence. Niosomes, type self-assembled non-ionic surfactant-based nanocarrier, have emerged as promising drug delivery system, offering improved stability, sustained release, enhanced bioavailability. In this study, niosomal nanocarriers encapsulating tiamulin were prepared, characterized assessed Mycoplasma-inoculated broilers following oral administration. Differential scanning colorimetry (DSC) confirmed alterations crystalline state components integration into structures formed during formulation procedure. Transmission electron microscopy (TEM) showed spherical nanostructure niosomes. The formulated exhibited zeta average hydrodynamic diameter −10.65 ± 1.37 mV 339.67 30.88 nm, respectively. Assessment parameters gallisepticum-infected revealed ability increase bioavailability systemic exposure, marked by significantly higher area under curve (AUC) (p < 0.01) prolonged elimination half-life (T1/2) 0.05). Enhanced residence time are crucial factors maintaining concentrations at reduced doses frequencies. This approach provides viable strategy decrease risk subtherapeutic levels, thereby mitigating development antibiotic resistance. findings presented herein offer sustainable for efficient use antibiotics medicine.

Язык: Английский

Процитировано

0