Evaluating Safe Infrared Neural Stimulation Parameters: Calcium Dynamics and Excitotoxicity Thresholds in Dorsal Root Ganglia Neurons DOI Creative Commons
Parinaz Abdollahiyan, Kunyang Sui, Guanghui Li

и другие.

Journal of Neuroscience Methods, Год журнала: 2025, Номер unknown, С. 110484 - 110484

Опубликована: Май 1, 2025

As a promising neural stimulation technique, infrared (INS) has recently gained significant attention due to its ability stimulate neuronal activities without needing exogenous agents. NIR light is absorbed by water of the tissue producing local thermal effects. Therefore, INS suitable candidate for localized and targeted stimulation. However, despite wide variety research studies on applications, limited have focused identifying optimizing parameters avoid potential excitotoxicity. This study evaluates dorsal root ganglia (DRG) neurons' response under with varying intensities illumination time. Here, DRG neurons are cultured labeled CamkII-GCaMP6s virus. The were exposed laser pulses (2.01µm wavelength, different powers 2.5mW, 5mW, 7.5mW, 10mW) durations 300seconds 400seconds. was delivered through silica optical fiber aligned stabilized within free-space setup. Simultaneous INS, activity evaluated calcium imaging fluorescence microscope. method allowed real-time monitoring dynamics conditions, preparing an overview safe thresholds INS. It found that saturation happened in exposure (7.5mW 300seconds, representing In contrast, same time, lower (2.5mW 5mW) did not show signs or damage. Moreover, some networks, peripheral illuminated area revealed indirect activation, indicating inter-neuronal communication Compared previous explored use neurons, our work introduces systematic approach evaluate intensity-dependent while addressing critical issue injury. While earlier demonstrated modulate reduce electrical artifacts electrophysiological recordings, concerns regarding excitotoxicity damage remain insufficiently investigated. We examined range determine optimize photothermal impact. Furthermore, utilizing CamKII-GCaMP6s virus-modified we enhance sensitivity detecting influx, providing more precise evaluation responses here, provide knowledge identifies required parameters, particularly intensity time efficient concluded higher can cause injury, prolonged exposure. observed activation suggests connections, offering further insights into effects networks. These findings contribute valuable information towards neuromodulation methods clinical settings.

Язык: Английский

An Analytical Slicing of Polymeric Tubes via 3D-Printed Dual Cutter DOI

Hae Woon Choi,

Talal O. Alshammari, Jong-Hyun Kim

и другие.

3D Printing and Additive Manufacturing, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Язык: Английский

Процитировано

0
Evaluating Safe Infrared Neural Stimulation Parameters: Calcium Dynamics and Excitotoxicity Thresholds in Dorsal Root Ganglia Neurons DOI Creative Commons
Parinaz Abdollahiyan, Kunyang Sui, Guanghui Li

и другие.

Journal of Neuroscience Methods, Год журнала: 2025, Номер unknown, С. 110484 - 110484

Опубликована: Май 1, 2025

As a promising neural stimulation technique, infrared (INS) has recently gained significant attention due to its ability stimulate neuronal activities without needing exogenous agents. NIR light is absorbed by water of the tissue producing local thermal effects. Therefore, INS suitable candidate for localized and targeted stimulation. However, despite wide variety research studies on applications, limited have focused identifying optimizing parameters avoid potential excitotoxicity. This study evaluates dorsal root ganglia (DRG) neurons' response under with varying intensities illumination time. Here, DRG neurons are cultured labeled CamkII-GCaMP6s virus. The were exposed laser pulses (2.01µm wavelength, different powers 2.5mW, 5mW, 7.5mW, 10mW) durations 300seconds 400seconds. was delivered through silica optical fiber aligned stabilized within free-space setup. Simultaneous INS, activity evaluated calcium imaging fluorescence microscope. method allowed real-time monitoring dynamics conditions, preparing an overview safe thresholds INS. It found that saturation happened in exposure (7.5mW 300seconds, representing In contrast, same time, lower (2.5mW 5mW) did not show signs or damage. Moreover, some networks, peripheral illuminated area revealed indirect activation, indicating inter-neuronal communication Compared previous explored use neurons, our work introduces systematic approach evaluate intensity-dependent while addressing critical issue injury. While earlier demonstrated modulate reduce electrical artifacts electrophysiological recordings, concerns regarding excitotoxicity damage remain insufficiently investigated. We examined range determine optimize photothermal impact. Furthermore, utilizing CamKII-GCaMP6s virus-modified we enhance sensitivity detecting influx, providing more precise evaluation responses here, provide knowledge identifies required parameters, particularly intensity time efficient concluded higher can cause injury, prolonged exposure. observed activation suggests connections, offering further insights into effects networks. These findings contribute valuable information towards neuromodulation methods clinical settings.

Язык: Английский

Процитировано

0