Cowpea genetic diversity, population structure and genome-wide association studies in Malawi: insights for breeding programs

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 20, 2025

Introduction This study focuses on cowpea, a vital crop for smallholder farmers in sub-Saharan Africa, particularly Malawi. The research aimed to understand the genetic diversity and population structure of cowpea perform genome-wide association studies (GWAS) identify marker-trait associations (MTAs) yield related traits. These insights are intended support varietal development address agricultural challenges Methods A total 306 genotypes were characterized using single nucleotide polymorphism (SNP) markers morphological assessed effects genotype, location, their interactions Fixed Random Model Circulating Probability Unification (FarmCPU) algorithm was used significant MTAs. Results traits showed interaction effects. Genotypes MWcp24, MWcp47, MWcp2232, TVu-3524 yielded highest values. Grain positively correlated with peduncle length, seeds per pod, pods plant. Three distinct clusters identified based Genetic analysis revealed an average minor allele frequency 0.31, observed heterozygosity 0.06, gene 0.33. inbreeding coefficient 0.82, indicating high level inbreeding. Most variation (73.1%) found among within populations. Nine groups ancestral populations identified, which did not entirely overlap geographic origins. Sixteen MTAs linked six Discussion validation these MTAs, along diversity, offers valuable opportunities improvement through marker-assisted selection, addresses faced by Malawian farmers. identification thirty lines as key founder breeding programs Malawi, Mozambique, Tanzania is outcome. efforts aim develop more productive region, enhancing food security sustainability.

Язык: Английский

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Insights on the utilisation of tissue culture to aid new breeding techniques for cowpea (Vigna unguiculata L.) improvement

Frontiers in Horticulture, Год журнала: 2025, Номер 4

Опубликована: Фев. 7, 2025

Cowpea ( Vigna unguiculata L.) is an underutilised vegetable legume indigenous to and predominantly cultivated consumed in Africa. However, its reach agricultural production consumption has expanded globally. This resilient crop known for ability withstand various environmental stressors, making it suitable marginal systems commonly used by small-scale farmers. Although cowpea exhibits tolerance drought, notably sensitive salinity stress biotic agents. The degree of drought varies among different cultivars, which requires further research develop more varieties.The changing climate patterns associated uncertainties highlight the urgent need breed productive cultivars. Conventional plant breeding techniques have produced new varieties cowpeas, yet limited genetic diversity within cowpeas poses challenges future conventional efforts. New (NBTs), including gene editing tools, single base pair alterations, DNA methylation methods, offer promising alternatives accelerate improvement. such approaches are also faced with success organogenesis (OG) somatic embryogenesis (SE) tissue culture. review examines advances use culture enhance productivity resilience against abiotic stresses.

Язык: Английский

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Genetic Diversity Analysis and Core Germplasm Construction of Rubus chingii Hu

Plants, Год журнала: 2024, Номер 13(5), С. 618 - 618

Опубликована: Фев. 23, 2024

Hu is the only species that used for both edible and medicinal purposes among 194 of genus

Язык: Английский

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Genetic Dissection of Diverse Seed Coat Patterns in Cowpea through a Comprehensive GWAS Approach

Plants, Год журнала: 2024, Номер 13(9), С. 1275 - 1275

Опубликована: Май 5, 2024

This study investigates the genetic determinants of seed coat color and pattern variations in cowpea (Vigna unguiculata), employing a genome-wide association approach. Analyzing mapping panel 296 varieties with 110,000 single nucleotide polymorphisms (SNPs), we focused on eight unique patterns: (1) Red (2) Cream seed; (3) White (4) Brown/Tan coat; (5) Pink, (6) Black, (7) Browneye (8) Red/Brown Holstein. Across six GWAS models (GLM, SRM, MLM, MLMM, FarmCPU from GAPIT3, TASSEL5), 13 significant SNP markers were identified led to discovery 23 candidate genes. Among these, four specific genes may play direct role determining pigment. These findings lay foundational basis for future breeding programs aimed at creating aligned consumer preferences market requirements.

Язык: Английский

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Seed Phenotyping and Genetic Diversity Assessment of Cowpea (V. unguiculata) Germplasm Collection

Agronomy, Год журнала: 2023, Номер 13(1), С. 274 - 274

Опубликована: Янв. 16, 2023

Cowpea is a nutrient-rich staple legume and climate-resilient crop for vulnerable agroecosystems. However, the still remains underutilized, mainly due to its narrow genetic base, production often ravaged by aphid infestation outbreaks. Thus, diversity assessment detection of defense-related alleles are fundamental germplasm management utilization in breeding strategies support food safety climate change times. A collection 87 cowpea landraces sourced from Greece was subjected seed phenotyping, SSR genotyping screening presence aphid-resistance-conferring alleles. Significant species’ local revealed. The were grouped metapopulations based on their broader geographical origin. High amounts variation statistically significant differences detected among regarding morphological traits, color eye according MANOVA (Wilk’s λ = 0.2, p < 0.01) correlations revealed these features Pearson’s test (p 0.05). levels polymorphism metapopulations, ranging 59% (VuPop3) 82% (VuPop4). AMOVA that 93% molecular distributed each metapopulation. Further population structure analysis presumed existence two inferred populations, where A, 79% have cream/cream-brown coat, whereas B, 94% brown-ochre black-seeded. Molecular conferring resistance correspondence 12 resistant genotype TVu-2876. study highlights importance diversity, as source important agronomic efforts expand cultivation foster security agriculture sustainability diversification change.

Язык: Английский

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Genetic diversity evaluation of core collection gene bank using simple sequence repeat marker of Acorus calamus L.: An important aromatic species

Industrial Crops and Products, Год журнала: 2023, Номер 204, С. 117292 - 117292

Опубликована: Авг. 21, 2023

Язык: Английский

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Physiological characterization and bioactive compounds of promising accessions of cowpea (Vigna unguiculata L. Walp) in the Peruvian Amazon

Frontiers in Agronomy, Год журнала: 2024, Номер 6

Опубликована: Май 7, 2024

Cowpea production in the Peruvian Amazon is increasing due to its ability adapt diverse environments, contribution soil conservation, and versatility of uses. This crop particularly valuable human nutrition high nutritional quality nutraceutical properties bioactive compounds. The aim study was evaluate physiology cowpea plants accessions PER1005854, PER1005851, PER12645, under influence rice husk mulch, screw tree litter, oil palm fiber, as well determine compounds present grains. aims promote sustainable production, expose aspects grains, foster their consumption both locally nationally. experiment conducted from November 2022 March 2023, Yurimaguas, Loreto, Peru. application organic mulches demonstrates a significantly positive impact on growth during vegetative phase. effect possibly attributed increased water availability, mulch reduces evaporation. In particular, PER12645 PER1005851 exhibit desirable physiological characteristics for grain with short cycles 71 days higher yields 1141 1125 kg/ha respectively. contrast, accession PER1005854 distinguished by biomass reaching value 14497 kg/ha, relevant trait foliage conservation. compounds, such proteins, lipids, carbohydrates, are similar those found other legumes. However, antioxidant activity dark tegument cowpeas proved be superior, highlighted (black tegument) 26.3 μmolTG/g, (dark red 19.5 μmolTG/g. characteristic important consumption, it related combat oxidative stress body.

Язык: Английский

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Harnessing Crop Diversity through Genetics, Genomics and Phenomics Approaches

Plants, Год журнала: 2023, Номер 12(8), С. 1685 - 1685

Опубликована: Апрель 17, 2023

Developing resilient cultivars ensuring adequate productions will be the agriculture industry's primary challenge in coming decades to ensure food security, especially with climate change and a growing global population [...].

Язык: Английский

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Innovations in Assessment Approaches of Plant Genetic Diversity

Sustainable development and biodiversity, Год журнала: 2024, Номер unknown, С. 289 - 337

Опубликована: Янв. 1, 2024

Язык: Английский

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Genetic diversity and population structure of cowpea mutant collection using SSR and ISSR molecular markers

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Дек. 30, 2024

Cowpea is a seed legume, important for food and nutritional security in Africa's arid semi-arid zones. Despite its importance, cowpea experiencing loss of genetic diversity due to climate change. Therefore, this study aimed evaluate the variability 33 mutant collections using 20 SSR 13 ISSR markers. This analysis shows an average number alleles 5.15 6.7 ISSR. The highest distance based on Nei's index among subgroups was 0.740 value polymorphism information content varied from 0.02 0.23 0.05 0.36 Our results revealed high GD (0.126), Fst (0.513), Shannon (0.246). population structure showed 3 4 clusters markers, respectively. Genetic variation, as assessed by molecular variance, mostly indicates higher within than populations. hierarchical clustering combined markers grouped accessions into four groups, supporting STRUCTURE results. Additionally, combination provided better level diversity, structure, more effective determining relationship between collection. enhances our understanding organization collection which can be used breeding programs era

Язык: Английский

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