Saffron In Vitro Propagation: An Innovative Method by Temporary Immersion System (TIS), Integrated with Machine Learning Analysis

Horticulturae, Год журнала: 2024, Номер 10(5), С. 454 - 454

Опубликована: Апрель 30, 2024

The propagation of Crocus sativus L. relies exclusively on corm multiplication. As underground storage organs, corms are susceptible to a wide range pathogens, environmental stresses, and diseases, making traditional methods often ineffective with the loss valuable material. In vitro offers an alternative for saffron culture under controlled conditions. particular, innovative application Temporary Immersion System (TIS) represents technological advancement enhancing biomass production reduction in operational costs. current study utilized Plantform™ bioreactor propagate from ‘Abruzzo’ region (Italy), integrating machine learning models assess its performance. evaluation explants after 30, 60, 90 days showed marked improvement growth microcorm compared conventional semisolid medium, supported by analysis. Indeed, Random Forest algorithm revealed predictive accuracy R2 value 0.81 number, showcasing capability forecast outcomes effectively. These results confirm that applying TIS could lead economically viable, large within environment, irrespective seasonality. This first endeavor use technology enhance conjunction learning, suggesting approach cultivating high-value crops like saffron.

Язык: Английский

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The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers

Agronomy, Год журнала: 2024, Номер 14(8), С. 1782 - 1782

Опубликована: Авг. 14, 2024

Potatoes (Solanum tuberosum L.) constitute one of the most economically important annual crops. In terms tissue culture, potato microtubers (MTs) have a number advantages over conventional plants. These include their small size, which greatly facilitates storage, transport, and germplasm exchange compared to in vitro One effective solution for production mass propagation healthy MTs is use temporary immersion systems (TISs). this study, SETISTM system containing kinetin/gibberellic acid (GA)/indole-3-butyric (IBA) hormones, we investigated effects different nutrient media on morphological characteristics explants MTs. We determined optimal cycling duration (3 h) with an frequency 2 min. The results revealed that medium culturing single-node bioreactor was M7 kinetin (2 mg/L), GA (0.5 IBA mg/L). obtaining M1 (hormone-free) high concentration sucrose (9%) at 18 °C under dark growing conditions. Thus, universal medium, employed bioreactor, selected both domestic foreign varieties.

Язык: Английский

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Improving Rooting of Cattleya gaskelliana (N.E. Br.) B.S. Williams (Orchidaceae Juss.) under In Vitro Conditions

Biology Bulletin, Год журнала: 2025, Номер 52(1)

Опубликована: Фев. 1, 2025

Язык: Английский

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In vitro asymbiotic seed germination and micropropagation of Dendrobium heyneanum Lindl. – an endemic orchid of Western Ghats, India

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2024, Номер 157(2)

Опубликована: Апрель 18, 2024

Язык: Английский

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Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose

Brazilian Journal of Biology, Год журнала: 2023, Номер 83

Опубликована: Янв. 1, 2023

This study examines the in vitro growth and ex establishment of Brassavola tuberculata relation to micropropagation system sucrose concentration employed culture. A completely randomized experimental design was utilized, employing a 2 x 5 factorial arrangement. The period began with seedlings cultivated for 180 days, which were subsequently transferred Murashige Skoog culture media containing concentrations 0, 15, 30, 45, or 60 g L-1. cultures subjected two systems: conventional gas exchange. After 90 days cultivation, plants evaluated, transplanted into substrate, placed screened nursery cultivation. 300 survival initial characteristics assessed. allowing exchange up 30 L-1 enhanced shoot root propagated plants. No noticeable anatomical differences observed after among different systems used. In establishment, irrespective concentration, facilitating positively influenced all evaluated characteristics.

Язык: Английский

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Orchid Micropropagation Using Temporary Immersion Systems: A Review

Methods in molecular biology, Год журнала: 2024, Номер unknown, С. 227 - 244

Опубликована: Янв. 1, 2024

Язык: Английский

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In vitro propagation strategies of Puya chilensis as an alternative for obtaining new cysteine proteases

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2024, Номер 158(2)

Опубликована: Июль 29, 2024

Язык: Английский

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Bioactive compounds in Laelia speciosa (Orchidaceae) seedlings grown in temporary immersion bioreactor

Mexican Journal of Biotechnology, Год журнала: 2024, Номер 9(1), С. 19 - 32

Опубликована: Янв. 5, 2024

Laelia speciosa is an epiphytic orchid endemic to Mexico, enjoying special protected status due illegal sales and clandestine field extraction. Until now, in vitro cultivation of this plant has not been explored using temporary immersion bioreactor (TIB). L. seedlings were grown a TIB, the presence bioactive compounds released into liquid culture medium was determined. Bioactive such as antioxidants, total polyphenols, flavonoids identified. The maximum concentration polyphenols 2.29 ± 0.10 mg GAE/g, antioxidant capacity reached 33.7 %. Colorimetric tests revealed flavanones flavonols. Gas chromatography-mass spectrometry identified components 1,3-dihydroxyacetone dimer 5-(hydroxymethyl)-2-furancarbaldehyde. Fourier transform infrared spectroscopy confirmed medium. This work provides alternative method for obtaining while conserving seedlings.

Язык: Английский

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Conclusions and Perspectives on Plant Micropropagation in Temporary Immersion Systems

Methods in molecular biology, Год журнала: 2024, Номер unknown, С. 247 - 249

Опубликована: Янв. 1, 2024

Язык: Английский

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In vitro propagation of the orchid Phalaenopsis circus via organogenesis and somatic embryogenesis using protocorm and thin cell layer explants

Italian Botanist, Год журнала: 2024, Номер 18, С. 29 - 50

Опубликована: Сен. 27, 2024

Orchids of the genus Phalaenopsis have high economic value in floriculture industry and market potential for breeding programs. In vitro propagation makes it possible to clonally mass proliferate conserve this valuable plant. current research, efficient protocols, some reported first time, circus through organogenesis somatic embryogenesis (SE) are presented. We used protocorms obtained from seeds thin cell layers (TCLs) prepared leaves as explants. Explants were cultured on Murashige Skoog (MS) basal medium enriched with various concentrations combinations plant growth regulators. Protocorms media fortified 2,4-dichlorophenoxyacetic acid (2,4-D) combination N-phenyl-N´-1,2,3-thiadiazol-5-yl-urea (TDZ), α-naphthalene-acetic (NAA) N 6 -furfuryl adenine or kinetin (Kin) organogenesis, well 2,4-D NAA SE. These produced either protocorm-like bodies (PLBs) embryos. Results showed that highest number PLBs (75.0) was 1.0 mg l –1 2,4-D. Maximum embryos (12.3/explant) containing 0.5 together 2.0 NAA. The use transversal TCLs 2–3 explants supplemented IBA combined TDZ plantlets. Plantlets transferred pots acclimatized ambient greenhouse conditions 100% survival rate.

Язык: Английский

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