The Open Biotechnology Journal,
Год журнала:
2023,
Номер
17(1)
Опубликована: Окт. 10, 2023
Background:
Epidemiological
studies
have
shown
that
elevated
levels
of
air
pollutants
are
associated
with
various
adverse
health
effects,
including
infertility.
Objective:
We
aimed
to
assess
the
protective
effects
aqueous
Urtica
dioica
leaf
extract
against
benzo[a]pyrene
-induced
oxidative
damage
in
mouse
testis.
Methods:
Mice
exposed
were
treated
or
without
for
five
weeks,
and
changes
body
testes
weights,
messenger
RNA
activities
antioxidant
enzymes,
plasma
testosterone
levels,
sperm
characteristics,
testicular
tissue
histology
determined.
Results:
Exposure
remarkably
reduced
testis
expression
activity
increased
lipid
peroxidation,
decreased
count
motility,
affected
morphology
viability,
damaged
seminiferous
tubules.
Treatment
attenuated
stress
by
increasing
genes.
Further,
antioxidative
enzyme
activity,
enhanced
improved
Conclusion:
Aqueous
protects
from
could
potentially
reverse
Molecules,
Год журнала:
2023,
Номер
28(13), С. 4925 - 4925
Опубликована: Июнь 22, 2023
Chinese
yam
(Dioscorea
opposita
Thunb.
cv.
Tiegun),
a
type
of
homologous
medicinal
plant,
mainly
grows
in
sandy
soil
(SCY)
and
loessial
(LCY).
However,
the
effects
on
metabolites
SCY
LCY
remain
unclear.
Herein,
this
study
aims
to
comprehensively
elucidate
LCY.
A
UPLC-MS/MS-based,
widely
targeted
metabolomics
approach
was
adapted
compare
chemical
composition
total
988
were
detected,
including
443
primary
metabolites,
510
secondary
35
other
compounds.
Notably,
177
differential
(classified
into
12
categories)
identified
between
LCY;
among
them,
85.9%
(152
metabolites)
upregulated
significantly
increased
contents
such
as
38
lipids
6
nucleotides
derivatives,
well
some
36
flavonoids,
28
phenolic
acids,
13
alkaloids,
tannins.
The
results
indicate
that
can
improve
nutritional
value
D.
opposita.
Journal of Experimental Pharmacology,
Год журнала:
2024,
Номер
Volume 16, С. 71 - 80
Опубликована: Фев. 1, 2024
Background:
Erianthemum
aethiopicum
Wiens
and
Polhill
(Loranthaceae)
is
a
parasitic
plant
native
to
north
eastern
Africa
Ethiopia.
In
Ethiopia,
it
traditionally
used
treat
breast
swelling,
mastitis,
morning
illnesses
vomiting.
Objective:
This
study
aimed
screen
the
main
phytochemical
constituents;
determine
total
amounts
of
phenolics,
flavonoids,
tannins;
evaluate
antimicrobial
(against
Escherichia
coli,
Staphylococcus
sciuri,
Candida
glaebosa
Cryptococcus
albidus
)
antioxidant
DPPH
radical
ferric
ion)
activities
E.
leaves
extracts.
Methods:
Powdered
were
macerated
using
n
-hexane,
chloroform,
ethyl
acetate,
ethanol,
methanol.
All
crude
extracts
qualitatively
screened
for
identification.
The
phenolic,
flavonoid,
condensed
tannin
contents
methanol
determined
by
UV-Vis
spectrophotometry.
evaluated
their
activity
against
aforementioned
microbes
agar
disc
diffusion
broth
micro-dilution
techniques.
Chloroform,
also
ion
reduction
power
(FRAP)
assays.
Results:
Methanol
(17.56
±
16%)
ethanol
(16.45
19%)
showed
better
extraction
efficiency.
Flavonoids,
polyphenols,
tannins,
terpenoids,
saponins,
sterols
detected
in
all
highest
content
phenolics
(22.63
0.69
mgGAE/gDCE),
flavonoids
(5.38
0.52
mgCE/gDCE)
tannins
(39.18
38
mg
CE/g
DCE),
as
milligram
gallic
acid
catechin
per
gram
dried
extract,
recorded
methanolic
extract.
extract
presented
best
anti
-DPPH
strength
(IC
50
,
4.31
μg/mL)
(absorbance
0.71)
though
found
weak
compared
ascorbic
0.49
μg/mL
absorbance
0.93,
respectively).
Conclusion:
displayed
antifungal
both
strains
(minimum
inhibitory
concentration
values
12.5–
25
mg/mL),
whereas
they
have
negligible
tested
bacterial
strains.
report
provides
preliminary
information
further
investigation
isolate
potential
compounds.
Keywords:
assay,
FRAP
analysis,
minimum
The Open Biotechnology Journal,
Год журнала:
2023,
Номер
17(1)
Опубликована: Окт. 10, 2023
Background:
Epidemiological
studies
have
shown
that
elevated
levels
of
air
pollutants
are
associated
with
various
adverse
health
effects,
including
infertility.
Objective:
We
aimed
to
assess
the
protective
effects
aqueous
Urtica
dioica
leaf
extract
against
benzo[a]pyrene
-induced
oxidative
damage
in
mouse
testis.
Methods:
Mice
exposed
were
treated
or
without
for
five
weeks,
and
changes
body
testes
weights,
messenger
RNA
activities
antioxidant
enzymes,
plasma
testosterone
levels,
sperm
characteristics,
testicular
tissue
histology
determined.
Results:
Exposure
remarkably
reduced
testis
expression
activity
increased
lipid
peroxidation,
decreased
count
motility,
affected
morphology
viability,
damaged
seminiferous
tubules.
Treatment
attenuated
stress
by
increasing
genes.
Further,
antioxidative
enzyme
activity,
enhanced
improved
Conclusion:
Aqueous
protects
from
could
potentially
reverse
