Biological Guardians: Unveiling Microbial Solutions to Combat Cannabis sativa Fungal Pathogens

Stresses, Год журнала: 2025, Номер 5(1), С. 16 - 16

Опубликована: Фев. 17, 2025

Cannabis (Cannabis sativa L.) is one of the earliest cultivated crops and valued for its medicinal compounds, food, fibre, bioactive secondary metabolites. The rapid expansion cannabis industry has surpassed development production system knowledge. scientific community currently focuses on optimising agronomic environmental factors to enhance yield quality. However, cultivators face significant challenges from severe pathogens, with limited effective control options. principal diseases include root rot, wilt, bud powdery mildew, stunt disease, microorganisms that reduce post-harvest Sustainable management strategies involve utilising clean planting stocks, modifying conditions, implementing sanitation, applying fungal bacterial biological agents, drawing decades research other crops. Plant–microbe interactions can promote growth regulate metabolite production. This review examines recent literature pathogen in indoor using biocontrol agents. Specific morphological, biochemical, characteristics hinder implementation cannabis. Subsequent investigations should focus elucidating plant–microbe essential effectiveness methodologies cultivation systems.

Язык: Английский

---

Building a community-driven bioinformatics platform to facilitate Cannabis sativa multi-omics research

Gigabyte, Год журнала: 2024, Номер 2024

Опубликована: Окт. 18, 2024

Global changes in cannabis legislation after decades of stringent regulation and heightened demand for its industrial medicinal applications have spurred recent genetic genomics research. An international research community emerged identified the need a web portal to host cannabis-specific datasets that seamlessly integrates multiple data sources serves omics-type analyses, fostering information sharing. The Tripal platform was used public genome assemblies, gene annotations, quantitative trait loci maps, protein expression data, metabolic profiles their sample attributes. Single nucleotide polymorphisms were called using resequencing on three genomes. Additional applications, such as SNP-Seek MapManJS, embedded into Tripal. A multi-omics integration web-service Application Programming Interface (API), developed top existing modules, returns generic tables samples, properties values. Use cases demonstrate API’s utility various omics enabling researchers perform analyses efficiently. Availability implementation can be accessed at www.icgrc.info.

Язык: Английский

---

Integrated Management of Pathogens and Microbes in Cannabis sativa L. (Cannabis) under Greenhouse Conditions

Plants, Год журнала: 2024, Номер 13(6), С. 786 - 786

Опубликована: Март 10, 2024

The increased cultivation of high THC-containing Cannabis sativa L. (cannabis), particularly in greenhouses, has resulted a greater incidence diseases and molds that can negatively affect the growth quality crop. Among them, most important are root rots (Fusarium Pythium spp.), bud rot (Botrytis cinerea), powdery mildew (Golovinomyces ambrosiae), cannabis stunt disease (caused by hop latent viroid), range microbes reduce post-harvest quality. An integrated management approach to impact these diseases/microbes requires combining different approaches target reproduction, spread, survival associated pathogens, many which occur on same plant simultaneously. These will be discussed context developing an plan manage pathogens greenhouse-grown at stages development. include maintenance stock plants, propagation through cuttings, vegetative flowering. genotypes with tolerance or resistance various is very approach, as well pathogen-free plants. When combined cultural (sanitation, irrigation, monitoring for diseases) environmental (greenhouse climate modification), significant reduction pathogen development spread achieved. use preventive applications microbial biological control agents reduced-risk biorational products also all production jurisdictions where they registered use. promising strategies plants during greenhouse reviewed. Future areas research identified.

Язык: Английский

---

Omics approaches to understanding plant microbiome gene function

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 85 - 100

Опубликована: Янв. 1, 2025

Язык: Английский

---

Integrated Management of Pathogens and Microbes on <em>Cannabis sativa</em> L. (Cannabis) under Greenhouse Conditions

Опубликована: Фев. 6, 2024

The increased cultivation of high THC-containing Cannabis sativa L. (cannabis), particularly in greenhouses, has resulted a greater incidence diseases and molds that can negatively affect the growth quality crop. Among them, most important are root rots (Fusarium Pythium spp.), Botrytis bud rot (Botrytis cinerea), powdery mildew (Golovinomyces ambrosiae), cannabis stunt disease (caused by Hop latent viroid), range microbes reduce post-harvest quality. An integrated management approach to impact these diseases/microbes requires combining different approaches target reproduction, spread survival associated pathogens, many which occur on same plant simultaneously. These will be discussed context developing an plan manage pathogens greenhouse-grown at stages development. include maintenance stock plants, propagation through cuttings, vegetative flowering. genotypes with tolerance or resistance various is very approach, followed pathogen-free plants. When combined cultural (sanitation, irrigation, monitoring for diseases) environmental (greenhouse climate modification), significant reduction pathogen development achieved. use preventive applications microbial biological control agents reduced risk biorational products also all production jurisdictions where they registered use. promising strategies plants during greenhouse reviewed. Future areas research identified.

Язык: Английский

---

Industrial Hemp As a Multi-Purpose Crop: Last Achievements and Research in 2018−2023

Journal of Natural Fibers, Год журнала: 2024, Номер 21(1)

Опубликована: Июнь 27, 2024

Industrial hemp cultivation was restricted years ago in most countries, thus wiping out centuries of science and genetic resources. It is only since the 1990s that has been experiencing its renaissance, which nowadays driven by new strategies. The objective review to compile summarize latest research achievements. focus on from 2018 2023. VOSviewer software used define main areas trends. Most articles were discussed, among others determination average yield loss due water stress (8.7 Mg ha−1) low temperatures (0.4 ha−1); increase up 25% achieving rejecting smallest seeds; or definition optimum dose sewage sludge application (25 ha−1). Regarding composition properties: identification caflanone flavonoid (selective activity against certain neoplasms); 71 compounds (monoterpenes, sesquiterpenes CBs) EO; antioxidant capacity EO (63 mg TE g−1 438 g−1); comparison content monoicous, female male inflorescences (0.10%, 0.15% 0.07% respectively). current possibilities using, e.g. for phytoremediation bioenergy, presented. Also knowledge gaps future identified.

Язык: Английский

---

NLR- and mlo-Based Resistance Mechanisms against Powdery Mildew in Cannabis sativa

Plants, Год журнала: 2023, Номер 13(1), С. 105 - 105

Опубликована: Дек. 29, 2023

Powdery mildew (PM) is one of the most common Cannabis sativa diseases. In spite this, very few documented studies have characterized resistance genes involved in PM defense mechanisms, or sources natural genetic cannabis. The focus present work on two primary mechanisms for qualitative against PM. first based (R) by conserved nucleotide-binding site and/or leucine-rich repeat domains (NLRs). second involves susceptibility (S) genes, and particularly locus o (MLO) whose loss-of-function mutations seem to be a reliable way protect plants from infection. defenses are thus discussed, mainly detailing strategies these mechanisms. Emerging about this research topic also reported and, significant results, potential model cannabis plant–pathogen interactions proposed. Finally, innovative approaches, pyramiding multiple R as well engineering genome editing methods knocking out S obtain durable PM-resistant cultivars with broad-spectrum range.

Язык: Английский

---

The influence of drying and storage conditions on the volatilome and cannabinoid content of Cannabis sativa L. inflorescences

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер 416(16), С. 3797 - 3809

Опубликована: Май 3, 2024

Abstract The increasing interest in hemp and cannabis poses new questions about the influence of drying storage conditions on overall aroma cannabinoids profile these products. Cannabis inflorescences are subjected to shortly after harvest then different containers. These steps may cause a process rapid deterioration with consequent changes precious secondary metabolite content, negatively impacting product quality potency. In this context, work, investigation effects freeze vs tray three preservation compounds has been performed. A multi-trait approach, combining both solid-phase microextraction (SPME) two-dimensional gas chromatography coupled mass spectrometry (SPME-GC × GC–MS) high-performance liquid (HPLC), is presented for first time. This approach permitted obtain detailed characterisation whole matrix terms volatile cannabinoids. Moreover, multivariate statistical analyses were performed obtained data, helping show that useful preserve cannabinoid preventing decarboxylation acid cannabinoids, but leads loss which responsible aroma. Furthermore, among conditions, glass bottle seems more beneficial retention initial VOC compared open air dry closed high-density polyethylene box. However, condition causes formation neutral at expenses highly priced forms. work will contribute help define optimal produce valuable high-quality Graphical

Язык: Английский

---

Differential Expression Analysis of Cannabis sativa response to thermotherapy of Hops Latent Viroid (HLVd) infection and clearance of the viroid in Tissue Culture Micropropagation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 8, 2024

Abstract Increased farming and cultivation of Cannabis sativa is rapidly pushing ( L.) towards becoming a commercial agricultural commodity. Large-scale facilities maintain thousands clonal varieties recreational medicinal cannabis there strong market-driven motivation to pipeline clean healthy vigorously growing plants free pathogens. However, mass production high-capacity create an environment that susceptible highly transmissible pathogens infectious entities such as Hop Latent Viroid (HLVd). From nurseries facilities, it’s become increasingly important routine testing regimen ensure are HLVd-free environments. One method address the problem infected perform thermotherapy on C. explants under tissue culture conditions isolate multiply productive mature plants. We carried out novel using in 5 type III were HLVd positive document degree success treatment at RNA level. observed following we able decrease level tests select some unable clear viroid. Plants tested one-step RT-qPCR method, developed validated, part, along with this work, present results well exploratory transcriptome analysis internally variety, AnnaLee, which negative for treatment, explore possible genes interest viroid infection, clearance, mitigation. Author Summary Hops latent (HLVd) non-infected varieties. A procedure was conducted meristem maintained through micropropagation techniques. Total isolated from cultured plantlet stocks evaluated by real-time reverse transcriptase assay HLVd. Infection status post assessed, viroid-free subsequently tested. single thermotherapy-treated cultivar, Anna Lee selected transcriptomics, differentially regulated treated discussed.

Язык: Английский

---

Molecular Investigations to Improve Fusarium Head Blight Resistance in Wheat: An Update Focusing on Multi-Omics Approaches

Plants, Год журнала: 2024, Номер 13(16), С. 2179 - 2179

Опубликована: Авг. 6, 2024

Fusarium head blight (FHB) is mainly caused by

Язык: Английский

---