Cloning, In Silico Characterization, and Physiochemical Analysis of the Zea mays PR-1 Gene: Implications for Developing Fungi-Resistant Transgenic Plants
Abstract
Pathogenesis-related
protein
1
(PR-1)
is
a
critical
component
of
plant
defense
mechanisms,
exhibiting
significant
antifungal
activity.
This
study
focused
on
the
PR-1
from
Zea
mays
and
five
other
species
wheat,
barley,
rice,
oats,
rye
to
explore
their
physicochemical,
structural,
functional
characteristics.
The
gene
was
successfully
isolated
Z.
amplified
using
Polymerase
Chain
Reaction
(PCR).
Following
cloning
into
pET15b
vector,
expressed
in
BL21-DE3
(E.
coli )
cells.
Induction
with
1mM
Isopropyl
β-D-1-thiogalactopyranoside
(IPTG)
for
4
hours
at
37°C
led
successful
expression,
as
confirmed
by
12%
SDS-PAGE.
exhibited
resistance
against
broad
spectrum
oomycete
pathogens,
demonstrating
its
potential
developing
fungi-resistant
transgenic
plants.
revealed
that
proteins
different
shared
high
sequence
similarity,
identities
ranging
65–90%.
However,
physicochemical
properties
varied
based
origin.
cereals
(maize,
rice)
were
found
be
more
hydrophobic
compared
those
grasses
(oats
rye).
Structurally,
all
adopted
similar
three-dimensional
conformation,
featuring
central
beta-barrel
surrounded
alpha-helices.
Functional
assays
six
activity
diverse
array
fungal
pathogens.
mays ,
along
species,
shows
agricultural
biotechnology.
By
incorporating
these
genes
crop
plants,
it
possible
enhance
thereby
advancing
development
resilient
sustainable
systems.
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Сен. 27, 2024
Язык: Английский