Transcriptome and Metabolome Profiling Provide Insights into Flavonoid Biosynthesis and the Mechanism of Color Formation in Zanthoxylum bungeanum Maxim. DOI Creative Commons
Lin Yang, Lu Tian, Jingwei Shi

и другие.

Plants, Год журнала: 2025, Номер 14(4), С. 558 - 558

Опубликована: Фев. 12, 2025

The color of Zanthoxylum bungeanum Maxim. (Z. bungeanum) is a key quality indicator and factor limiting the development its industry. However, underlying mechanisms governing formation remain largely unexplored. In this study, an integrative analysis transcriptome metabolome profiles was conducted across four developmental stages to elucidate mechanism in Z. bungeanum. A total 137 flavonoids were identified as fruits ripened, with high levels differentially accumulated metabolites (DAMs), including tricetin (-)-epigallocatechin, which strongly associated formation. This suggests their significant contribution pigmentation process. Nine expressed genes (DEGs) candidate involved development. Additionally, 15 transcription factors (TFs) (12 MYB 3 bHLH) exhibited expression patterns similar those structural flavonoid biosynthetic pathway, indicating role regulating synthesis. bioinformatics three synthesis genes-ZbCHI, ZbFLS, ZbANR-revealed that all proteins exhibit hydrophobic structures without transmembrane domains. Among them, ZbANR possesses signal peptide regions, whereas ZbCHI ZbFLS do not. Subcellular localization predictions suggest most likely localized chloroplast, cytoplasm, membrane. Functional analyses revealed transient Nicotiana benthamiana (N. benthamiana) increased content, overexpression producing distinct white phenotype plants. study enriches transcriptomic data provides comprehensive understanding metabolism molecular basis bungeanum, offering valuable theoretical foundation for future breeding programs.

Язык: Английский

Transcriptome and Metabolome Profiling Provide Insights into Flavonoid Biosynthesis and the Mechanism of Color Formation in Zanthoxylum bungeanum Maxim. DOI Creative Commons
Lin Yang, Lu Tian, Jingwei Shi

и другие.

Plants, Год журнала: 2025, Номер 14(4), С. 558 - 558

Опубликована: Фев. 12, 2025

The color of Zanthoxylum bungeanum Maxim. (Z. bungeanum) is a key quality indicator and factor limiting the development its industry. However, underlying mechanisms governing formation remain largely unexplored. In this study, an integrative analysis transcriptome metabolome profiles was conducted across four developmental stages to elucidate mechanism in Z. bungeanum. A total 137 flavonoids were identified as fruits ripened, with high levels differentially accumulated metabolites (DAMs), including tricetin (-)-epigallocatechin, which strongly associated formation. This suggests their significant contribution pigmentation process. Nine expressed genes (DEGs) candidate involved development. Additionally, 15 transcription factors (TFs) (12 MYB 3 bHLH) exhibited expression patterns similar those structural flavonoid biosynthetic pathway, indicating role regulating synthesis. bioinformatics three synthesis genes-ZbCHI, ZbFLS, ZbANR-revealed that all proteins exhibit hydrophobic structures without transmembrane domains. Among them, ZbANR possesses signal peptide regions, whereas ZbCHI ZbFLS do not. Subcellular localization predictions suggest most likely localized chloroplast, cytoplasm, membrane. Functional analyses revealed transient Nicotiana benthamiana (N. benthamiana) increased content, overexpression producing distinct white phenotype plants. study enriches transcriptomic data provides comprehensive understanding metabolism molecular basis bungeanum, offering valuable theoretical foundation for future breeding programs.

Язык: Английский

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