New Insights Into Tissue Culture Plant-Regeneration Mechanisms

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Июнь 30, 2022

Plant regeneration occurs when plants repair or replace damaged structures based on the totipotency and pluripotency of their cells. Tissue culture is one most widely used regenerative technologies. Recently, a series breakthroughs were made in study plant regeneration. This review summarizes two pathways tissue culture: somatic embryogenesis de novo organogenesis. Furthermore, we environmental factors influencing from explant sources, basal medium, growth regulators, light/dark treatment. Additionally, analyse molecular mechanisms underlying pathways. knowledge will promote an understanding fundamental principles precursor cells lay solid foundation for applying micropropagation genetic modification.

Язык: Английский

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Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration

Developmental Cell, Год журнала: 2022, Номер 57(4), С. 526 - 542.e7

Опубликована: Янв. 20, 2022

Highlights•The dynamic chromatin state landscape during shoot regeneration is revealed•Collapse of pluripotency in the absence cytokinin signaling•Generation a catalog regulatory sequences regeneration•A cohort transcription factors involved outlinedSummaryShoot mediated by sequential action two phytohormones, auxin and cytokinin. However, landscapes underlying this response have not yet been studied. In study, we jointly profiled accessibility, histone modifications, transcriptomes to demonstrate that high auxin/cytokinin ratio environment primes Arabidopsis increasing accessibility gene loci associated with fate determination. Cytokinin signaling only triggers commitment progenitor at later stages but also allows maintain identity genes priming stage. Our analysis transcriptional dynamics further identifies cis-elements dedicated cell transitions uncovers important roles BES1, MYC, IDD, PIF regeneration. results, thus, provide comprehensive resource for studying reprogramming plants potential targets improving future efficiency.Graphical abstract

Язык: Английский

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Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

Science China Life Sciences, Год журнала: 2024, Номер 67(7), С. 1338 - 1367

Опубликована: Май 31, 2024

Язык: Английский

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The Past, Present and Future of Cannabis sativa Tissue Culture

Plants, Год журнала: 2021, Номер 10(1), С. 185 - 185

Опубликована: Янв. 19, 2021

The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies the species. Micropropagation affords researchers producers methods to rapidly propagate insect-/disease-/virus-free clonal plants store germplasm forms basis other biotechnologies. Despite this need, research area is limited due long history prohibitions restrictions. Existing literature multiple limitations: publications use hemp as proxy drug-type when it well established that there significant genotype specificity; studies using cultivars are predominantly optimized single cultivar; most protocols have not been replicated by independent groups, some attempts demonstrate lack reproducibility across genotypes. Due culture decline problems, multiplication phase micropropagation (Stage 2) fully developed reports. This review will provide brief background on botany comprehensive critical summary tissue culture. Special attention be paid current challenges faced researchers, limitations existing studies, developments future directions technologies.

Язык: Английский

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An Improved Agrobacterium-Mediated Transformation and Genome-Editing Method for Maize Inbred B104 Using a Ternary Vector System and Immature Embryos

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Май 4, 2022

For maize genome-editing and bioengineering, genetic transformation of inbred genotypes is most desired due to the uniformity background in their progenies. However, lines are recalcitrant tissue culture transformation. A public, transformable B104 has been widely used for genome editing recent years. This primarily its high degree similarity shared with B73, an reference parent many breeding populations. Conventional protocol requires 16–22 weeks produce rooted transgenic plants average 4% frequency (number T0 per 100 infected embryos). In this Method paper, we describe advanced that only 7–10 generate 6.4% frequency. Over 66% carried CRISPR/Cas9-induced indel mutations on target gene, demonstrating can be applications. Following detailed stepwise procedure described here, quick simplified method using Agrobacterium ternary vector system consisting a T-DNA binary compatible helper plasmid readily transferable interested researchers.

Язык: Английский

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Developmental processes in the Rosaceae through the lens of DNA and RNA methylation

Planta, Год журнала: 2025, Номер 261(3)

Опубликована: Фев. 8, 2025

This review discusses the DNA and RNA methylation pathways their biological roles in Rosaceae developmental processes relevant for breeding production. The is a plant family of great importance human nutrition health. Many traits are influenced by epigenetic methylation, functions which now being unravelled several important species this family. Methylation at 5th position cytosine (5mC) well-established mark that affects cellular such as gene expression genome stability involved wide range functions. Further to this, recent technological advances have uncovered other naturally occurring chemical modifications additional layers regulatory information plants. In we give comprehensive summary 5-methylcytosine mechanisms components identified We detail discuss role 5mC dynamics processes, including phase transition, bud development, dormancy, architecture, regeneration, fruit ripening senescence. then understanding newly nucleic acid modifications, N6-adenosine (6mA) (m6A) mechanisms. summarise adenosine emerging modification development findings Rosaceous species. Integrating aspects with genetics physiology crucial

Язык: Английский

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Recent advances in understanding of the epigenetic regulation of plant regeneration

aBIOTECH, Год журнала: 2023, Номер 4(1), С. 31 - 46

Опубликована: Янв. 16, 2023

Ever since the concept of "plant cell totipotency" was first proposed in early twentieth century, plant regeneration has been a major focus study. Regeneration-mediated organogenesis and genetic transformation are important topics both basic research modern agriculture. Recent studies model

Язык: Английский

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Cytokinin Signaling and De Novo Shoot Organogenesis

Genes, Год журнала: 2021, Номер 12(2), С. 265 - 265

Опубликована: Фев. 12, 2021

The ability to restore or replace injured tissues can be undoubtedly named among the most spectacular achievements of plant organisms. One such regeneration pathways is organogenesis, formation individual organs from nonmeristematic tissue sections. process triggered in vitro by incubation on medium supplemented with phytohormones. Cytokinins are a class phytohormones demonstrating pleiotropic effects and powerful network molecular interactions. present study reviews existing knowledge possible sequence genetic events behind de novo shoot organogenesis initiated cytokinins. Overall, review aims collect reactions encompassed cytokinin primary responses, starting phytohormone perception dedicated receptors, transcriptional reprogramming cell fate last module multistep-phosphorelays. It also includes brief reminder other control mechanisms, as epigenetic reprogramming.

Язык: Английский

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Genetic Transformation of Recalcitrant Upland Switchgrass Using Morphogenic Genes

Frontiers in Plant Science, Год журнала: 2022, Номер 12

Опубликована: Фев. 8, 2022

Switchgrass (Panicum virgatum) is an excellent feedstock for biofuel production. While genetic transformation routinely done in lowland switchgrass, upland cultivars remain recalcitrant to transformation. Here we report the establishment of efficient and reproducible protocol two cultivars, 'Summer' 'Blackwell', by ectopic overexpression morphogenic genes, Baby boom (Bbm) Wuschel2 (Wus2). Two auxotrophic Agrobacterium strains, LBA4404Thy- EHA105Thy-, each harboring same construct containing ZmBbm, ZmWus2, a green fluorescence protein (GFP) gene, ZsGreen1, were used infect immature leaf segments derived from vitro grown seedlings. The strains also contain helper plasmid that carry additional copies virulence genes. GFP-expressing calli identified selected regeneration. highest efficiency 6% was obtained tetraploid cultivar Summer when infection, which twice octoploid Blackwell. consistently outperformed EHA105Thy- on frequency across cultivars. Fifteen randomly putative transgenic plants Blackwell, representing independent callus events, confirmed as presence transgene, ZmAls, absence AtuFtsZ, chromosomal gene specific strain LBA4404 using polymerase chain reaction. Transgene integration expression further detection GFP roots, resistance herbicide injury leaves plants. ZmBbm ZmWus2 genes successfully removed 40 33.3% respectively, via Cre-Lox recombination system upon heat treatment embryogenic calli. Our successful switchgrass provides method function analysis germplasm enhancement biotechnology.

Язык: Английский

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GWAS supported by computer vision identifies large numbers of candidate regulators of in planta regeneration in Populus trichocarpa

G3 Genes Genomes Genetics, Год журнала: 2024, Номер 14(4)

Опубликована: Фев. 7, 2024

Abstract Plant regeneration is an important dimension of plant propagation and a key step in the production transgenic plants. However, capacity varies widely among genotypes species, molecular basis which largely unknown. Association mapping methods such as genome-wide association studies (GWAS) have long demonstrated abilities to help uncover genetic trait variation plants; however, performance these depends on accuracy scale phenotyping. To enable large-scale GWAS planta callus shoot model tree Populus, we developed phenomics workflow involving semantic segmentation quantify regenerating tissues over time. We found that resulting statistics were highly non-normal distributions, thus employed transformations or permutations avoid violating assumptions linear models used GWAS. report 200 statistically supported quantitative loci (QTLs), with genes encompassing near top QTLs including regulators cell adhesion, stress signaling, hormone signaling pathways, well other diverse functions. Our results encourage hormonal during consider keystone roles stress-related (e.g. jasmonates salicylic acid), addition auxin cytokinin pathways commonly considered. The putative regulatory biological processes identified provide new insights into complexity regeneration, may serve reagents for improving transformation recalcitrant species.

Язык: Английский

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