Copper Hexacyanoferrates Obtained via Flavocytochrome b2 Assistance: Characterization and Application

Biosensors, Год журнала: 2025, Номер 15(3), С. 157 - 157

Опубликована: Март 2, 2025

Artificial enzymes or nanozymes (NZs) are gaining significant attention in biotechnology due to their stability and cost-effectiveness. NZs can offer several advantages over natural enzymes, such as enhanced under harsh conditions, longer shelf life, reduced production costs. The booming interest is likely continue potential applications expand. In our previous studies, we reported the “green” synthesis of copper hexacyanoferrate (gCuHCF) using oxidoreductase flavocytochrome b2 (Fcb2). Organic–inorganic micro-nanoparticles were characterized detail, including structure, composition, catalytic activity, electron-mediator properties. An SEM analysis revealed that gCuHCF possesses a flower-like structure well-suited for concentrating stabilizing Fcb2. As an effective peroxidase (PO) mimic, has been successfully employed H2O2 detection amperometric sensors oxidase-based biosensors. current study, demonstrated uniqueness lies its multifunctionality, serving PO chemosensor ammonium ions, biosensor L-lactate, exhibiting perovskite-like This exceptional ability enhance fluorescence blue light irradiation being first time. Using PO-like NZ, novel developed, optical L-arginine electrochemical biosensors methanol glycerol determination. Thus, gCuHCF, synthesized via Fcb2, presents promising platform development biosensors, bioreactors, biofuel cells, solar other advanced devices. innovative approach utilizing biocatalysts nanoparticle highlights groundbreaking direction materials science biotechnology.

Язык: Английский

Detection of Ammonia Nitrogen in Neutral Aqueous Solutions Based on In Situ Modulation Using Ultramicro Interdigitated Array Electrode Chip

Chemosensors, Год журнала: 2025, Номер 13(4), С. 138 - 138

Опубликована: Апрель 9, 2025

In this study, an in situ electrochemical modulation method based on ultramicro interdigitated array electrode (UIAE) sensor chip was developed for the detection of ammonia nitrogen (NH3-N) neutral aqueous solutions. One comb UIAE used as working both modulating and sensing functions, while other counter electrode. Utilizing its enhanced mass transfer proximity effects, feasibility solution environment near to generate response NH3-N investigated using methods. The proposed enhances concentration hydroxide ions active chloride local chip. These reactive species play a key role improving sensor’s electrocatalytic oxidation capability toward nitrogen, facilitating sensitive environments. A linear relationship displayed, ranging from 0.15–2.0 mg/L (as nitrogen) with sensitivity 3.7936 µA·L·mg−1 (0.0664 µA µM−1 mm−2), which 2.45 times that strong alkaline conditions without modulation. Additionally, relative standard deviation measurement remained below 2.9% over five days repeated experiments, indicating excellent stability.

Язык: Английский

Moringa oleifera mediated MnO2 electrochemical sensor for ammonia detection in aqueous medium

Journal of Materials Science Materials in Electronics, Год журнала: 2024, Номер 35(34)

Опубликована: Дек. 1, 2024

Язык: Английский