Optimization of Polylactide-Co-Glycolide-Rifampicin Nanoparticle Synthesis, In Vitro Study of Mucoadhesion and Drug Release DOI Open Access
Nazgul A. Yessentayeva, Aldana R. Galiyeva, Arailym T. Daribay

и другие.

Polymers, Год журнала: 2024, Номер 16(17), С. 2466 - 2466

Опубликована: Авг. 30, 2024

Despite the large number of works on synthesis polylactide-co-glycolide (PLGA) nanoparticles (NP) loaded with antituberculosis drugs, data influence various factors final characteristics complexes are quite contradictory. In present study, a comprehensive analysis effect multiple factors, including molecular weight PLGA, size and stability nanoparticles, as well loading efficiency release drug rifampicin (RIF), was carried out. Emulsification out using different surfactants (polyvinyl alcohol, Tween 80 Pluronic F127), aqueous-to-organic phase ratios, solvents (dichloromethane, dimethyl sulfoxide, ethyl acetate). this research, PLGA nanoemulsion formation process accompanied by ultrasonic dispersion, at frequencies durations homogenization. The use central composite design method made it possible to select optimal conditions for preparation PLGA-RIF NPs (particle 223 ± 2 nm, 67 1%, yield 47 2%). from studied first time flow cell vertical diffusion Franz cells pH levels, simulating gastrointestinal tract. For purpose inhalation administration immobilized in NPs, their mucoadhesion mucin studied, high degree adhesion polymeric mucosa shown (more than 40% within 4 h). example strain H37Rv vitro, sensitivity Mycobacterium tuberculosis proven complete inhibition growth.

Язык: Английский

Central Composite Design for Formulation and Optimization of Rifampicin Loaded Polylactide-Co-Glycolide Nanoparticles DOI Open Access
Nazgul A. Yessentayeva, Aldana R. Galiyeva,

D. T. Sadyrbekov

и другие.

Опубликована: Июнь 24, 2024

The aim of this study was to synthesize and optimize polylactide-co-glycolide (PLGA) nanoparticles loaded with rifampicin (RIF), a given size high loading rate, using the central composite design (CCD) method. CCD used investigate influence independent factors such as PLGA:RIF ratio, type PLGA, concentration surfactants, power duration homogenization, organic solvent its ratio aqueous phase on dependent physicochemical characteristics nanoparticles. optimized were investigated scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) differential calorimetry (DSC). Comparative evaluation drug release kinetics carried out different pH setups: flow cuvette (USP 4 apparatus) Franz diffusion cuvette. In addition, mucoadhesive properties mycobacterial activity PLGA-RIF NPs studied.

Язык: Английский

Процитировано

0
Optimization of Polylactide-Co-Glycolide-Rifampicin Nanoparticle Synthesis, In Vitro Study of Mucoadhesion and Drug Release DOI Open Access
Nazgul A. Yessentayeva, Aldana R. Galiyeva, Arailym T. Daribay

и другие.

Polymers, Год журнала: 2024, Номер 16(17), С. 2466 - 2466

Опубликована: Авг. 30, 2024

Despite the large number of works on synthesis polylactide-co-glycolide (PLGA) nanoparticles (NP) loaded with antituberculosis drugs, data influence various factors final characteristics complexes are quite contradictory. In present study, a comprehensive analysis effect multiple factors, including molecular weight PLGA, size and stability nanoparticles, as well loading efficiency release drug rifampicin (RIF), was carried out. Emulsification out using different surfactants (polyvinyl alcohol, Tween 80 Pluronic F127), aqueous-to-organic phase ratios, solvents (dichloromethane, dimethyl sulfoxide, ethyl acetate). this research, PLGA nanoemulsion formation process accompanied by ultrasonic dispersion, at frequencies durations homogenization. The use central composite design method made it possible to select optimal conditions for preparation PLGA-RIF NPs (particle 223 ± 2 nm, 67 1%, yield 47 2%). from studied first time flow cell vertical diffusion Franz cells pH levels, simulating gastrointestinal tract. For purpose inhalation administration immobilized in NPs, their mucoadhesion mucin studied, high degree adhesion polymeric mucosa shown (more than 40% within 4 h). example strain H37Rv vitro, sensitivity Mycobacterium tuberculosis proven complete inhibition growth.

Язык: Английский

Процитировано

0