Journal of Molecular Structure, Год журнала: 2023, Номер 1302, С. 137396 - 137396
Опубликована: Дек. 29, 2023
Язык: Английский
BMC Microbiology, Год журнала: 2024, Номер 24(1)
Опубликована: Июль 9, 2024
Abstract Various eco-friendly techniques are being researched for synthesizing ZnO-NPs, known their bioactivity. This study aimed at biosynthesizing ZnO-NPs using Streptomyces baarnensis MH-133, characterizing physicochemical properties, investigating antibacterial activity, and enhancement of efficacy by combining them with a water-insoluble active compound (Ka) in nanoemulsion form. Ka is pure 9-Ethyl-1,4,6,9,10-pentahydroxy-7,8,9,10-tetrahydrotetracene-5,12-dione obtained previously from our strain MH-133. Biosynthesized employing MH-133 filtrate zinc sulfate (ZnSO 4 .7H 2 O) as precursor were purified characterized investigation. High-resolution-transmission electron microscopy (HR-TEM) verified the effective biosynthesis (size < 12 nm), whereas dynamic light scattering (DLS) analysis showed an average size 17.5 nm. X-ray diffraction (XRD) exhibited characteristic patterns that confirmed crystalline structure. efficiently inhibited both Gram-positive Gram-negative bacteria (MICs: 31.25–125 µg/ml). The was combined to improve effectiveness reduce dose checkerboard microdilution. Niteen treatments combinations matrix Klebsiella pneumonia . Eleven had fractional inhibitory concentration index (FICi) between 1.03 2, meaning indifferent, another five resulted additive FICi (0.625–1) only one combination 0.5, indicating synergy. In case methicillin-resistant S. aureus (MRSA), Ka-ZnO-NPs yielded 23 varying degrees interaction. results eleven indifferent interaction, eight interactions, two synergies 0.5 0.375. synergy action transformed into form solubility bioavailability. HR-TEM revealed spherical oil particles granulated core smaller than 200 nm no signs aggregation. Effective dispersion DLS which indicated droplets have 53.1 polydispersity (PI) 0.523. killing kinetic assay assessed viability Staphylococcus ( MRSA) K. post-treatment either non-formulated or Results show time-dependent manner, higher findings without formulation MIC values killed after 24 h but not MRSA. Our loaded mentioned value bactericidal effect 18 incubation against MRSA , respectively, compared free combinations. At half value, increased activity cause bacteriostatic on incubation. impact 6 before regrew increase log 10 colony forming unit (CFU)/ml over initial level. Similarly, cytotoxicity decreased kidney epithelial cells African green monkey (VERO) cell line. IC 50 treatment 8.17/1.69 (µg/µg)/ml, nano-emulsion, it 22.94 + 4.77 (µg/µg)/mL. conclusion, efficient may be promising solution fighting ESKAPE pathogenic according low toxicity.
Язык: Английский
Процитировано
15
Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)
Опубликована: Янв. 22, 2024
Abstract Nanosilver oxide exhibits strong antibacterial and photocatalytic properties has shown great application potential in food packaging, biochemical fields, other fields involving diseases pest control. In this study, Ag 2 O nanoparticles were synthesized using Bacillus thuringiensis (Bt-Ag NPs). The physicochemical characteristics of the Bt-Ag NPs analyzed by UV‒vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), inductively coupled plasma emission spectrometry (ICP), high-resolution transmission microscopy (HR-TEM), zeta potential. phis-chemical characterization revealed that are spherical shape with small particle size (18.24 nm), high crystallinity, well dispersity, stability. biopesticidal antifungal effects tested against Tribolium castaneum , Aspergillus flavus Penicillium chrysogenum . survival, growth, reproduction pests molds significantly inhibited a dose-dependent manner. showed higher pesticidal activities T. than Bt commercial NPs. LC 50 values Bt, NPs, 0.139%, 0.072%, 0.06% on day 14, respectively. also A. P. while it resulted inhibition zone did. addition, much more sensitive to NP treatments, compared Our results B. could act as pesticides agents stored-product fields. Key points • be (Bt) degree shape, excellent insecticidal activity
Язык: Английский
Процитировано
7
Agronomy, Год журнала: 2025, Номер 15(1), С. 211 - 211
Опубликована: Янв. 16, 2025
This research study investigated the production and properties of zinc oxide (ZnO) nanoparticles derived from corn husks their priming effects on wheat plant proliferation antioxidant mechanisms compared to nutri-priming technique under regular irrigation drought-stressed conditions. Transmission scanning electron microscopy (TEM SEM), energy-dispersive X-ray spectroscopy (EDAX), diffraction confirmed nanoparticles’ hexagonal morphology typical dimensions 51 nm. The size stability these were assessed through distribution zeta potential analysis, indicating reasonable stability. Fourier-transform infrared (FTIR) detected newly formed functional groups. emphasized role reactive oxygen species (ROS) phenolic compounds in responses nanoparticle treatment, particularly detoxifying harmful radicals. also examined activity enzymes, including peroxidase (POX), catalase (CAT), glutathione reductase (GR), alleviating stress caused by oxidation while subjected various treatments, micronutrient seed with DR GREEN fertilizer. Some biochemical compounds, such as total phenolics (TPCs), flavonoids (TFCs), hydrolysable sugars, estimated well show effect different treatments plants. findings suggested that ZnO can enhance enzyme certain conditions posing phytotoxic risks, underscoring complexity plant–nanoparticle interactions for improving crop resilience targeted applications.
Язык: Английский
Процитировано
1
Inorganic and Nano-Metal Chemistry, Год журнала: 2024, Номер unknown, С. 1 - 17
Опубликована: Май 27, 2024
The green synthesis of silver nanoparticles (AgNPs) using various natural resources is one the emerging fields nanotechnology. Researchers are investigating eco-friendly ways to synthesize after receiving a lot attention while ago for AgNPs. Synthesis nanomaterials and utilizing bottom-up top-down methods. This assessment consequently covers broad categories potential health concerns as well physical, chemical, processes. use dangerous chemicals or their high costs, respectively, have made many chemical physical methods less common today. producers would greatly benefit from this work's full understanding approaches analytical characterizing AgNPs antibacterial mechanisms action, applications antimicrobial, antifungal, antibacterial, antiviral, anticancer, other effects, covered all significant aspects
Язык: Английский
Процитировано
4
Chemical Research in Chinese Universities, Год журнала: 2025, Номер unknown
Опубликована: Март 18, 2025
Язык: Английский
Процитировано
0
Biotechnology Reports, Год журнала: 2025, Номер unknown, С. e00896 - e00896
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Ноя. 14, 2024
The anticancer, antimicrobial, and insecticidal activities of sarcotrocheliol (1) cholesterol (2) obtained from the soft coral Sarcophyton trocheliophorum (S. trocheliophorum) were intensively studied. According to this study, both compounds 1 2 showed potential cytotoxicity towards human colorectal carcinomaHCT-116 (IC50 10.4, 11.8 µg/mL) liver carcinoma HepG2 cell lines 8.8, 12.0 µg/mL), respectively. Compounds evaluated as inhibitors caspase-3, a member cysteine protease family, which is considered key enzyme in inducing apoptosis. Results that have induced apoptosis via up-regulation caspase-3. Sarcotrocheliol displayed antimicrobial activity against P. aeruginosa (15 mm), B. subtilis M. luteus (14 mm) C. albicans with MIC 1.5 µg/mL reported test microorganisms. On other hand, less (10 S. aureus (12 MICs 3.0, 1.5, 3.0 tested microorganisms, Larvicidal revealed remarkable toxicity third instar larvae mosquito, Culex pipiens even at concentration ppm. Adulticidal data are distinctly potent toxicants housefly, Musca domestica adult females. Overall, compound 2induced much more than 1, females sensitive larvae. Computationally, Density Functional Theory (DFT) analyses had higher dipole moment lower band gap energy when compared 1. So, chemically reactive stable molecular docking study PDB IDs: 3KJF, 5UHF 1ACJ, demonstrated their mode agents. exerted many interactions high binding proteins, recognizing drug candidates broad bioactivities.
Язык: Английский
Процитировано
3
Marine Drugs, Год журнала: 2023, Номер 21(8), С. 437 - 437
Опубликована: Авг. 2, 2023
The marine environment is a rich source of bioactive compounds. Therefore, the sea cucumber was isolated from Red Sea at Al-Ain Al-Sokhna coast and it identified as surf redfish (Actinopyga mauritiana). aqueous extract utilized an ecofriendly, novel sustainable approach to fabricate zinc oxide nanoparticles (ZnO-NPs). biosynthesized ZnO-NPs were physico-chemically characterized evaluated for their possible antibacterial insecticidal activities. Additionally, safety in non-target organism model (Nile tilapia fish) also investigated. spherical with average size 24.69 ± 11.61 nm had peak 350 shown by TEM UV-Vis, respectively. XRD analysis indicated crystalline phase 21.7 nm. FTIR pattern showed biological residues extract, highlighting potential role biosynthesis process. DLS negative zeta (−19.2 mV) which good preliminary indicator stability. larvicidal activity against mosquito Culex pipiens (LC50 = 15.412 ppm LC90 52.745 ppm) potent adulticidal effect housefly Musca domestica (LD50 21.132 LD90 84.930 ppm). Tested concentrations strong 3rd larval instar. Topical assays revealed dose-dependent M. after 24 h treatment ZnO-NPs. presented wide two fish-pathogen bacteria, Pseudomonas aeruginosa Aeromonas hydrophila. Histopathological hematological investigations organism, Nile fish exposed 75–600 provide impacts. Overall, data highlighted applications redfish-mediated effective safe way control mosquitoes, houseflies pathogenic bacteria.
Язык: Английский
Процитировано
3
Journal of Molecular Structure, Год журнала: 2023, Номер 1302, С. 137396 - 137396
Опубликована: Дек. 29, 2023
Язык: Английский
Процитировано
3