Development of an extraction method of mucuna pruriens seeds for a chemically and physically stable extract DOI Creative Commons

Chayarit Vilairat

Опубликована: Янв. 1, 2022

Levodopa (L-DOPA) is a drug for the treatment of Parkinson's disease (PD). Currently, it derives from chemical synthesis and plants, especially Mucuna Pruriens (MP) seeds.� The results clinical studies found using L-DOPA-containing MP �for PD could reduce side effects more than synthetic one but patients must take powder in high amounts per single dose which may cause inconvenience. Therefore, extract can help reduction dose. Unfortunately, water seeds be easily degraded physically chemically; physical appearance e.g., darkening color, melting, or solid lump. Moreover, L-DOPA content was commonly observed. necessary to develop an extraction procedure solve these problems. This study aimed modify traditional acidification approach obtain higher stable by comparing efficiency set previously studied acid solutions (hydrochloric acid, citric ascorbic acid) Phyllanthus emblica (PE), has antioxidant also useful treating as well. From comparative two strains seeds, that amount levodopa not statistically different. While sonication-assisted method improve efficiency. When different used extraction, PE yielded with similar hydrochloric control solution, helps maintain quality when stored under accelerated condition 12 months. In addition, qualitative analysis chemicals PEW extracted HPTLC HPLC techniques revealed did interfere PEW. All showed beneficial process safe natural solvent. obtained starting material herbal products.

Язык: Английский

Green Synthesis of Eosin-Y Coated Silver Nanoparticles for Sensitive and Selective Fluorometric Detection of L-Dopa DOI

Karuppiah Nagaraj,

Pilavadi Thangamuniyandi,

Gunasekaran Velmurugan

и другие.

Journal of Fluorescence, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Язык: Английский

Процитировано

1
Electroanalytical Overview: The Determination of Levodopa (L-DOPA) DOI Creative Commons
Robert D. Crapnell, Craig E. Banks

ACS Measurement Science Au, Год журнала: 2023, Номер 3(2), С. 84 - 97

Опубликована: Фев. 3, 2023

L-DOPA (levodopa) is a therapeutic agent which the most effective medication for treating Parkinson's disease, but it needs dose optimization, and therefore its analytical determination required. Laboratory instruments can be routinely used to measure are not always available in clinical settings traditional research laboratories, they also have slow result delivery times high costs. The use of electroanalytical sensing overcomes these problems providing highly sensitivity, low-cost, readily portable solution. Consequently, we overview reported throughout literature summarizing endeavors toward L-DOPA, offer insights into future opportunities.

Язык: Английский

Процитировано

15
Synergistic dopa-reinforced fluid hydrosol as highly efficient coal dust suppressant DOI
Xia Tong, Zhilin Xi,

Lianquan Suo

и другие.

Chemical Engineering Journal, Год журнала: 2023, Номер 479, С. 147641 - 147641

Опубликована: Ноя. 23, 2023

Язык: Английский

Процитировано

14
Dual Enzyme-mimicking Bimetallic MOF for Selective SERS Detection of L-DOPA in Human Serum Based on Cascade Catalytic Reaction DOI

Shuojiang Liu,

Ying Chen,

Xing-Liang Cheng

и другие.

Talanta, Год журнала: 2025, Номер 294, С. 128178 - 128178

Опубликована: Апрель 17, 2025

Язык: Английский

Процитировано

0
Sublingual Crushed Levodopa for Parkinson’s Disease Management of a Lewy Body Dementia Patient With Dysphagia: A Case Report DOI Open Access

Kristin Regehr,

Jessica Yu, Fiona Wu

и другие.

Cureus, Год журнала: 2025, Номер unknown

Опубликована: Май 29, 2025

Язык: Английский

Процитировано

0
An Amperometric Biosensor Based on a Bilayer of Electrodeposited Graphene Oxide and Co-Crosslinked Tyrosinase for L-Dopa Detection in Untreated Human Plasma DOI Creative Commons

Giuseppa Cembalo,

Rosanna Ciriello,

Carmen Tesoro

и другие.

Molecules, Год журнала: 2023, Номер 28(13), С. 5239 - 5239

Опубликована: Июль 6, 2023

L-Dopa, a bioactive compound naturally occurring in some Leguminosae plants, is the most effective symptomatic drug treatment for Parkinson’s disease. During disease progression, fluctuations L-DOPA plasma levels occur, causing motor complications. Sensing devices capable of rapidly monitoring would allow adjusting L-Dopa dosing, improving therapeutic outcomes. A novel amperometric biosensor detection described, based on tyrosinase co-crosslinked onto graphene oxide layer produced through electrodeposition. Careful optimization enzyme immobilization procedure permitted to improve long-term stability while substantially shortening and simplifying fabrication. The effectiveness protocol combined with enhanced performances electrodeposited allowed achieve high sensitivity, wide linear range, limit 0.84 μM, suitable within its window. Interference from endogenous compounds, tested at concentrations typically found drug-treated patients, was not significant. Ascorbic acid exhibited inhibitory behavior therefore rejected enzymatic by casting an outer Nafion membrane. proposed device applied human plasma, showing good recoveries.

Язык: Английский

Процитировано

8
Plier Ligands for Trapping Neurotransmitters into Complexes for Sensitive Analysis by SERS Spectroscopy DOI Creative Commons
Olga E. Eremina, Olesya O. Kapitanova, Алексей В. Медведько

и другие.

Biosensors, Год журнала: 2023, Номер 13(1), С. 124 - 124

Опубликована: Янв. 11, 2023

Catecholamines-dopamine, noradrenaline and adrenaline are important biomarkers of neurotransmitter metabolism, indicating neuroendocrine tumors neurodegenerative diseases. Surface-enhanced Raman spectroscopy (SERS) is a promising analytical technique with unprecedented multiplexing capabilities. However, not all analytes exhibit strong SERS signals on stable robust nanostructured substrates. In this work, we propose novel indicator system based the formation mixed ligand complexes bispidine-based bis-azole ligands which can serve as pliers to trap Cu(II) ions stabilize its catecholamines. Four synthesized different functional groups: carboxyl, amino, benzyl, methoxybenzyl, were applied for forming shift maximum absorbance catecholamines from ultraviolet region 570-600 nm. A new band in visible range resonates local surface plasmon resonance (LSPR) metal nanoparticles most used laser wavelengths. This match allowed use Molecular Immobilization Resonant Amplification by Complex-Loaded Enhancers (MIRRACLE) methodology measure intense silver-based SERS-active substrate. The plier-like fixed stabilized catecholamine sensor surface, facilitated determination dopamine 3.2 × 10-12-1 10-8 M concentration range.

Язык: Английский

Процитировано

7
A Critical Overview of Enzyme-Based Electrochemical Biosensors for L-Dopa Detection in Biological Samples DOI Creative Commons

Carmen Tesoro,

Giuseppa Cembalo,

Antonio Guerrieri

и другие.

Chemosensors, Год журнала: 2023, Номер 11(10), С. 523 - 523

Опубликована: Окт. 5, 2023

L-Dopa is an intermediate amino acid in the biosynthesis of endogenous catecholamines, such as dopamine. It currently considered to be optimal dopaminergic treatment for Parkinson’s disease, a neurodegenerative disorder affecting around 1% population. In advanced stage complications dyskinesia and psychosis are caused by fluctuations plasma drug levels. Real-time monitoring levels would advantageous properly adjusting dosing, thus improving therapeutic efficacy. Electrochemical methods have advantages easy-to-use instrumentation, fast response time, high sensitivity, suitable miniaturization, enabling fabrication implantable or wearable devices. This review reports on research papers past 20 years (2003–2023) dealing with enzyme-based biosensors electrochemical detection biological samples. Specifically, amperometric voltammetric biosensors, whose output signal measurable current, discussed. The approach adopted includes initial study steps required assemble devices, i.e., electrode modification enzyme immobilization. Then, all issues related their analytical performance terms selectivity, capability analyze real samples critically paper aims provide assessment recent developments while highlighting limitations poor selectivity long-term stability, laborious time-consuming protocol that needs addressed from perspective integrated clinical management disease.

Язык: Английский

Процитировано

6
Traditional analytical methods in food industry: Current challenges and issues in food analysis DOI

Insha Kaloo,

Saadiya Naqash,

Darakshan Majid

и другие.

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 1 - 22

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

2
Distribution of Vicine, Convicine and Levodopa in Faba Bean Plant Tissues Determined by Ultrahigh Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry DOI Creative Commons

Devini De Silva,

Yong Liu, Mark Smith

и другие.

Journal of Chromatography Open, Год журнала: 2024, Номер 5, С. 100127 - 100127

Опубликована: Март 12, 2024

Faba bean is an emerging pulse crop for the Canada Prairies and becoming increasingly important source of plant sourced protein. It also has a high content levodopa (up to 0.1% dry seed weight), nutraceutical compound treating Parkinson's Disease (PD). The presence pyrimidine glycosides, vicine convicine, however, restricts use faba due their potential cause favism in susceptible individuals. For rapid separation quantification levodopa, 5-min ultrahigh performance liquid chromatography-electrospray ionization mass spectrometry method (UHPLC-ESI MS) was developed. This provided wide linear range (4.8 nM 60 µM vicine, 6.0 24 convicine levodopa), low limit detection (1.2 3.0 nM). applied analysis diverse samples, from dehulled mature coat, fresh tissues such as flowers, leaves, stems, roots pods. results indicated that tissues, leaves had highest concentration level VC, which makes them alternative plant-based L-DOPA with food-based treatment Parkinsons Disease.

Язык: Английский

Процитировано

2