Опубликована: Янв. 1, 2024
Язык: Английский
Plant and Soil, Год журнала: 2024, Номер unknown
Опубликована: Окт. 21, 2024
Abstract Background Despite more than 50 years of research, no robust evidence suggests that inoculation cereals and other non-legumes with free-living and/or endophytic bacteria leads to fixation agronomically significant quantities dinitrogen gas (N 2 ) from the atmosphere. A plethora new products claims increase growth yields major crops through stimulating N -fixation by inoculating bacteria. Scope We review literature on in rhizosphere as endophytes non-legume plants. find unequivocal these fix agriculturally amounts atmosphere non-legumes. Research since 1930s has followed repeated, overlapping cycles have concluded plant-growth-promoting hormones were primary reason for crop response microbial inoculants. Conclusion contend regulations are required prevent sale inoculant unsubstantiated spurious claims. Such should require is provided independently verified, that: (i) bacterium can (i.e. it possesses all genes make nitrogenase), (ii) a clear mechanism protect nitrogenase poisoning free oxygen, (iii) present sufficient numbers throughout cycle plant, (iv) enhanced respiration be detected putative -fixing tissues, (v) growing an N-free medium prolific accumulation nitrogen, (vi) one method used confirm quantitatively inputs field.
Язык: Английский
Процитировано
8
Horticulturae, Год журнала: 2025, Номер 11(1), С. 80 - 80
Опубликована: Янв. 13, 2025
Biological nitrogen (N) fixation has been advocated in agricultural fields due to being considered a more sustainable way introduce N into agrosystems than industrial fertilizers. In this study, foliar spray inoculant containing the microorganism Methylobacterium symbioticum was applied. This is known for fixing phyllosphere, regardless of cultivated species. study conducted three rainfed olive orchards over years. two managed according European Union (EU) integrated production rules, experiment organized as factorial design with (applied at levels, yes and no) fertilization soil 0, 40, 80 kg ha−1 N). The third trial, EU organic farming completely randomized treatments: (yes) without (no) treatment involving seaweed extract, also application. microbiological did not consistently influence yield or concentration leaves across trials. Conversely, application significantly influenced yield. one trials, year (80 ha−1) resulted an ~eight times higher unfertilized control treatment. extract lead significant differences leaf mineral composition compared other treatments. These findings from on-farm research highlight importance accurately determining conditions under which commercial products can deliver effective results. It crucial acknowledge that these involve expenses only their acquisition but
Язык: Английский
Процитировано
1
Nitrogen, Год журнала: 2025, Номер 6(1), С. 2 - 2
Опубликована: Янв. 3, 2025
This study presents the results of two on-farm trials evaluating efficacy a nitrogen (N)-fixing inoculant (Methylobacterium symbioticum) applied as foliar spray to provide N hazelnut (Corylus avellana L.) and walnut (Juglans regia trees. In trial, factorial design was employed with soil application at three levels [0 (N0), 40 (N40), 80 (N80) kg ha−1] (Yes No). The trial arranged completely randomized treatments: N-fixing microorganism, seaweed extract, control. Soil significantly increased yield in 2021 (1.99, 2.49, 2.65 t ha−1 for N0, N40, N80, respectively) but not 2022 (average values ranging from 0.28 0.33 ha−1). did affect yield. no significant differences were observed among treatments either year. average yields ranged 1.72 2.38 0.66 0.84 2022. hazelnuts tended increase leaf concentration kernel concentration. one sampling dates walnuts, years. extract influence or None consistently affected other macronutrients micronutrients leaves. Therefore, while showed some potential improve nutritional status trees, it Overall, sufficiently compelling, indicating need further studies on these species before commercial product can be confidently recommended farmers.
Язык: Английский
Процитировано
0
Agronomy, Год журнала: 2025, Номер 15(2), С. 442 - 442
Опубликована: Фев. 11, 2025
Plant biostimulants are used to promote plant growth by increasing tolerance abiotic stressors and improving the efficiency of natural resource use. In present two-year research (2022–2023 2023–2024), effects biostimulant foliar applications on morphological parameters, fresh dry yields, antioxidant capacity, total phenolic content, chemical composition essential oil thyme (Thymus vulgaris L.) were studied. For this purpose, four commercial biostimulants, Biostimol Plus + Peptamin-V Plus®, Acadian MPE®, Megafol®, BlueN®, evaluated cultivated in field conditions. The experiment was laid out a randomized block design with five treatments three replications. During second growing season, plants treated composed bacteria Methylobacterium symbioticum SB23, showed highest weight (152.1 g plant−1), biomass yield (501.9 m−2), (172.2 m−2). BlueN® that also obtained both years (0.47 0.53%), for all amount thymol carvacrol increased year, especially Megafol® (63.75 3.16%). capacity enhanced year according ABTS assay, but particular, BPPVP (26.97 μmol/g 25.01 μmol/g), while content higher first (65.98 mg GAE/g Extract). other had less intense effects. conclusion, influenced some characteristics oil, greatest influencers BPPVP.
Язык: Английский
Процитировано
0
Agronomy, Год журнала: 2025, Номер 15(3), С. 521 - 521
Опубликована: Фев. 21, 2025
This study aims to evaluate the potential of a nitrogen-fixing endophyte, Methylobacterium symbioticum SB23, as sustainable biofertilizer in peach (Prunus persica) cultivation. We compared three treatments: control with soil application ammonium sulfate (250 kg ha−1) and two endophyte foliar applications at different doses: single high-dose (M.SYM500 500 split half-dose (M.SYM250 250 ha−1 applied twice). The first was made fruit stage’s appearance, second when fruits were fully developed. Key assessments included chlorophyll content shoot growth, which evaluated 52 100 days after (DAA), continued growth benefits observed through 193 DAA. Evaluations conducted characteristics, amino acid profiles, plant tissues leaves for nitrogen phosphorus 107 DAA (harvest). M.SYM500 treatment notably enhanced weight increased specific acids, such glutamic acid, methionine, phenylalanine, contributing improved quality resistance properties. No significant differences °Brix (total soluble solid) levels among treatments, indicating that photosynthetic gains likely directed towards biomass structural rather than sugar accumulation. demonstrates endophytes can be effective reducing reliance on synthetic fertilizers while sustaining or improving quality.
Язык: Английский
Процитировано
0
Insects, Год журнала: 2025, Номер 16(4), С. 412 - 412
Опубликована: Апрель 14, 2025
The root-associated microbiome significantly influences plant health and pest resistance, yet the temporal dynamics of its compositional functional change in response to Ectropis grisescens Warren (Lepidoptera: Geometridae) infestation remain largely unexplored. study took samples leaves, roots, rhizosphere soil at different times after plants were attacked by E. grisescens. These analyzed using transcriptomic high-throughput sequencing 16S rRNA techniques. goal was understand how plant’s defense mechanisms microbial community around roots changed attack. Additionally, bacterial feedback assays conducted evaluate effects selected strains on growth responses. By conducting collected samples, we found significant shifts communities seventh day, suggesting a lag adaptation. Transcriptomic analysis revealed that attack induced reprogramming tea root transcriptome, upregulating genes related defensive pathways such as phenylpropanoid flavonoid biosynthesis. Metagenomic data indicated changes microbiome, with enrichment linked metabolic nitrogen cycling. Network showed reorganization core members, favoring nitrogen-fixing bacteria like Burkholderia species. Bacterial confirmed strains, notably cepacia strain ABC4 (T1) nine-strain consortium (T5), enhanced responses, including elevated levels flavonoids, polyphenols, caffeine, jasmonic acid, increased peroxidase (POD) superoxide dismutase (SOD) activities. This emphasizes potential utilizing for sustainable management cultivation, thereby enhancing resilience crops while maintaining ecosystem balance.
Язык: Английский
Процитировано
0
Sustainability, Год журнала: 2025, Номер 17(8), С. 3505 - 3505
Опубликована: Апрель 14, 2025
In recent years, a significant increase in the market availability of products with phytostimulant effect on plants has occurred. However, these are not always low-cost, and their effects crops reproducible. this study, an alternative use lavender, already known for its antimicrobial activity, is proposed: aqueous extract from self-produced lavender (Lavandula angustifolia Mill., var. Hidcote) flowering tops was tested activity lettuce (Lactuca sativa L., Bionda d’estate) cultivated under organic farming management. Lettuce were planted open field private farm (in Lazio region, Italy): treated weekly two months extracts while control sprayed water. Results showed that treatment enhanced fresh edible production dry biomass (12.08% 15.09%, respectively) plants, as well leaf area index (28.01%) photosynthetic efficiency (increased SPAD). At same time, mineral content observed: compared to control, 30.46% observed N, 31.10%, 35.52%, 36.19%, 47.51%, 48.11%, 91.44% K, Ca, Mg, P, Mn, Fe, respectively. All factors contribute enhancing commercial nutritional quality lettuce, strengthening self-defense extending shelf life. study exhibits could be useful product obtaining higher yield better farming.
Язык: Английский
Процитировано
0
BioTech, Год журнала: 2024, Номер 13(3), С. 32 - 32
Опубликована: Авг. 21, 2024
Seven auxin-producing endophytic bacterial strains (Azospirillum spp., Methylobacterium symbioticum, Bacillus spp.), and two different combinations of these were used to verify their influence on tomato during germination development in hydroponic conditions where, as a novelty for Canestrino di Lucca cultivar, bacteria inoculated. To emphasize the presence auxins roots stems seedlings, both situ staining qualitative assessment quantitative analysis carried out. Moreover, hypogeal epigeal growth plantlets measured, correlation analyses conducted examine relationship between amount indolacetic acid (IAA) produced by root stem parameters. Plantlets treated with microbial inoculants showed significant increase survival rate compared control treatment. The best results IAA producers from Azospirillum baldaniorum Sp245 A. brasilense Cd, which also induced growth. On other hand, amyloliquefaciens B. licheniformis rates These findings highlight potential using co-cultivation system that enables inoculating plantlets, at an early stage (5 days old).
Язык: Английский
Процитировано
3
Seeds, Год журнала: 2024, Номер 3(3), С. 479 - 492
Опубликована: Сен. 5, 2024
The use of microbial seed priming may be a promising tool to improve the first stages germination several herbaceous species. In tomatoes (Solanum lycopersicum L.), enhanced and vigor, biotic abiotic stress control, with reduction in chemicals, have been reported. this study, seeds from two Italian tomato varieties (Canestrino di Lucca Pisanello) were primed seven different strains plant growth-promoting rhizobacteria (PGPB) belonging Azospirillum baldaniorum, A. brasilense, Methylobacterium symbioticum, Bacillus amyloliquefaciens, B. licheniformis, subtilis. They selected for their ability produce auxin. test was carried out on treated percentage calculated. obtained seedlings transplanted kept greenhouse conditions. After 60 d, fresh dry weight, root number, length plantlets recorded. A general significant improvement growth parameters observed plants. All proved indolacetic acid (IAA) producers using Salkowsky method. positive relationship between number length, amount IAA found. overall results suggest that could useful advancing organic farming, sustainable agriculture, environmental protection.
Язык: Английский
Процитировано
3
Plants, Год журнала: 2024, Номер 13(20), С. 2909 - 2909
Опубликована: Окт. 17, 2024
In this study, the effectiveness of an inoculant containing a nitrogen (N)-fixing microorganism (
Язык: Английский
Процитировано
3