Reviving hope: Phage therapy application for antimicrobial resistance in farm animal production over the past decade

Animal Feed Science and Technology, Год журнала: 2025, Номер unknown, С. 116333 - 116333

Опубликована: Апрель 1, 2025

Язык: Английский

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Antimicrobial resistance in global catfish aquaculture: Trends, control strategies, and public health implications

Aquaculture, Год журнала: 2025, Номер unknown, С. 742552 - 742552

Опубликована: Апрель 1, 2025

Язык: Английский

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Regulating antimicrobial resistance in the environment: analysis of Thailand legal framework and areas for reinforcement

Science in One Health, Год журнала: 2025, Номер unknown, С. 100110 - 100110

Опубликована: Апрель 1, 2025

Язык: Английский

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Emerging contaminants and their behavior in aquifer systems

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 17 - 30

Опубликована: Янв. 1, 2025

Язык: Английский

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Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis—A Targeted, Blood Culture-Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5463 - 5463

Опубликована: Май 17, 2024

Sepsis is a life-threatening syndrome triggered by infection and accompanied high mortality, with antimicrobial resistances (AMRs) further escalating clinical challenges. The rapid reliable detection of causative pathogens AMRs are key factors for fast appropriate treatment, in order to improve outcomes septic patients. However, current sepsis diagnostics based on blood culture limited low sensitivity specificity while molecular approaches fail enter routine. Therefore, we developed suppression PCR-based selective enrichment sequencing approach (SUPSETS), providing method combining multiplex PCR Nanopore identify most common sepsis-causative using plasma cell-free DNA. Applying only 1 mL plasma, targeted eight across three kingdoms ten proof-of-concept study. SUPSETS was successfully tested an experimental research study the first samples revealed comparable results metagenomics clearly outperforming culture. Several clinically relevant could be additionally detected. Furthermore, provided pathogen AMR-specific reads within minutes starting sequencing, thereby potentially decreasing time-to-results 11–13 h suggesting diagnostic potential sepsis.

Язык: Английский

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River waters in Greece: A reservoir for clinically relevant extended-spectrum-β-lactamases-producing Escherichia coli

The Science of The Total Environment, Год журнала: 2024, Номер 941, С. 173554 - 173554

Опубликована: Май 31, 2024

Язык: Английский

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Polyphenol Contents, Antibacterial and Antioxidant Effects of Four Palestinian Honey Samples, and their Anticancer Effects on Human Breast Cancer Cells

Journal of Pure and Applied Microbiology, Год журнала: 2024, Номер 18(2), С. 1372 - 1385

Опубликована: Июнь 1, 2024

The phenolic compounds of four Palestinian honey samples (PH1-PH4) and their antibacterial effects as well cytotoxic, cytostatic, antimigration in human breast cancer cell line (MDA) were evaluated here. HPLC analysis PH2 (Cornflower), PH3 (Milk thistle), PH4 (Ziziphus) revealed 15 compounds, namely, caffeic acid, carvacrol, chrysin, ellagic galangin, gallic kaempferol, p-coumaric pinobanksin, pinocembrin, protocatechuic quercetin, rutin, salicylic silydamin. minimum inhibitory concentration (MIC) method applied to Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli). A strong positive correlation was detected between antimicrobial activity coli) IC50 values for DPPH neutralization varied from 0.19 w/w% 10 w/w%. potential anticancer properties the on MDA cells. Samples demonstrated cytostatic activity, reducing viability by about 43% at non-toxic 4 mg/mL. strongly correlated with presence acid (p < 0.01). Moreover, migration rate significantly reduced (by up 85%) compared untreated cells 0.05). observed carvacrol Pinocembrin Our findings validate honey’s suggest its benefits may stem effects.

Язык: Английский

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Quaternary Treatment of Urban Wastewater for Its Reuse

Processes, Год журнала: 2024, Номер 12(9), С. 1905 - 1905

Опубликована: Сен. 5, 2024

In today’s ongoing rapid urban expansion, deforestation and climate changes can be observed mainly as unbalanced rain occurrence during the year, long seasons without any at all unordinary high temperatures. These adverse affect underground water levels availability of surface water. addition, quite a significant proportion drinking is used for non-drinking purposes. With several EU countries increasingly suffering from droughts, reusing quaternary treated wastewater help address scarcity. At European level, Regulation 2020/741 Parliament Council 25 May 2020 on minimum requirements reuse was adopted. This regulation foresees use recycled agricultural irrigation. article provides an overview various processes, such filtration, coagulation, adsorption, ozonation, advanced oxidation processes disinfection, treatment in order to remove micropollutants achieve reuse. According literature, most effective method with acceptable financial costs combination membrane filtration (UF or NF) UV disinfection. are relatively well known commercially available. also helps researchers identify key themes concepts, evaluate strengths weaknesses previous studies determine areas where further research needed.

Язык: Английский

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Plastic Polymers and Antibiotic Resistance in an Antarctic Environment (Ross Sea): Are We Revealing the Tip of an Iceberg?

Microorganisms, Год журнала: 2024, Номер 12(10), С. 2083 - 2083

Опубликована: Окт. 17, 2024

Microbial colonization of plastic polymers in Antarctic environments is an under-investigated issue. While several studies are documenting the spread pollution Ross Sea, whether formation a plastisphere (namely complex microbial assemblage colonizing plastics) may favor antibiotic-resistant bacteria (ARB) this marine environment unknown yet. A experiment was performed ecosystem, aiming at exploring potential role as reservoir antibiotic resistance. To end, biofilm-producing activity and susceptibility profiles bacterial strains isolated from biofilms submerged polyvinylchloride polyethylene panels were screened. The carried out two different sites namely Road Bay Tethys Bay. Most isolates able to produce biofilm; multidrug resistances detected members associated PVC PE (also named plastisphere), well surrounding water. lowest percentage ARB found PE-associated not-impacted (control) Punta Stocchino station, whereas highest one PVC-associated station. However, no selective enrichment relation study or either type material observed, suggesting that resistance antibiotics generalized widespread phenomenon. Resistance against all three classes assayed (i.e., cell wall antibiotics, nucleic acids, protein synthesis inhibitors) observed. high showing remote like ones, suffering increasing anthropic pressure, points emerging threat with pathogenic risk needs further deepening studies.

Язык: Английский

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Oil spill pollution and diversity analyses of resistant bacteria isolated from soil across the Arabian Sea and Bay of Bengal coastlines

Environmental Monitoring and Assessment, Год журнала: 2024, Номер 196(12)

Опубликована: Ноя. 27, 2024

Язык: Английский

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