Evaluation of a point-of-use device used for autoantibody analysis and its potential for following microcystin leucine-arginine exposure DOI Creative Commons
Hui Ma, Christine E. Loscher, Anne Parle‐McDermott

и другие.

Frontiers in Sensors, Год журнала: 2024, Номер 5

Опубликована: Фев. 9, 2024

Introduction: Globally, the need for measuring exposure to algal toxins has become urgent due ever-increasing reports of contamination in sea and freshwater, shellfish fish stocks aerosols. Methods: To address this issue, we evaluated potential determining autoantibodies a panel biomarkers known be elevated following hepatotoxin microcystin leucine-arginine. The presence autoantibodies, specific four selected stress-response, metabolomic chaperone biomarkers, namely, Heat shock protein 1, Triosephosphate isomerase, Peroxiredoxin 1 2 was employed screening 371 serum samples from microcystin-exposed individuals Tanzania. In addition, capacity LightDeck fluorescence-based detector, point-of-use device, monitor these autoantibody responses comparison enzyme-linked immunosorbent assay evaluated. Results: By using determination novel an altered response observed exposure, with levels generally upregulated. leucine-arginine water, as well food sources Tanzania, may potentially have significant health effects on population. Discussion: This biomarker detection various exposure-associated cancers (e.g., hepatocellular cancer colorectal cancer). utilisation device proved successful rapid analysis panel.

Язык: Английский

The cytotoxicity of microcystin-LR: ultrastructural and functional damage of cells DOI

Kangfeng Ge,

Xingde Du, Haohao Liu

и другие.

Archives of Toxicology, Год журнала: 2024, Номер 98(3), С. 663 - 687

Опубликована: Янв. 22, 2024

Язык: Английский

Процитировано

11
Astaxanthin Alleviates Hepatic Lipid Metabolic Dysregulation Induced by Microcystin-LR DOI Creative Commons

Qinmei Tan,

Hanyu Chu,

Jia Wang

и другие.

Toxins, Год журнала: 2024, Номер 16(9), С. 401 - 401

Опубликована: Сен. 18, 2024

Microcystin-LR (MC-LR), frequently generated by cyanobacteria, has been demonstrated to raise the likelihood of liver disease. Few previous studies have explored potential antagonist against MC-LR. Astaxanthin (ASX) shown possess various beneficial effects in regulating lipid metabolism liver. However, whether ASX could alleviate MC-LR-induced hepatic metabolic dysregulation is as yet unclear. In this work, important roles and mechanisms countering damage were for first time. The findings revealed that not only prevented weight loss but also enhanced health after MC-LR exposure. Moreover, effectively decreased triglyceride, total cholesterol, aspartate transaminase, alanine aminotransferase contents mice elevated Histological observation showed significantly alleviated accumulation inflammation induced Mechanically, diminish expression genes responsible generation (

Язык: Английский

Процитировано

11
Hydrogeochemical Characterization and Source Evaluation of Groundwater Contamination in Parts of the Uddanam CKDu Area, Southern India: Integrating Statistical, Geochemical Modeling, Pollution, and Health Indices DOI

Vinod Kumar Yarlanki,

Gope Naik Vadithya,

Balaji Etikala

и другие.

International Journal of Environmental Research, Год журнала: 2025, Номер 19(3)

Опубликована: Янв. 11, 2025

Язык: Английский

Процитировано

2
Sphingopyxis sp. YF1 and metabolite astaxanthin alleviate MC-LR-induced kidney injury DOI
Yafang Li, Jia Wang, Xiaofang Liang

и другие.

Process Safety and Environmental Protection, Год журнала: 2025, Номер unknown, С. 106982 - 106982

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Synergistic reproductive toxicity of microcystin-LR and polystyrene micro/nano-plastics in male zebrafish DOI

Jiayao Duan,

Yuchun Xiao,

Wenxin Wang

и другие.

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 299, С. 118377 - 118377

Опубликована: Май 26, 2025

Язык: Английский

Процитировано

0
Research progress of microcystin-LR toxicity to the intestine, liver, and kidney and its mechanism DOI Creative Commons
Yaqi Wang,

Xiaodie Zhou,

Yue Yang

и другие.

Environment International, Год журнала: 2025, Номер 201, С. 109547 - 109547

Опубликована: Май 29, 2025

Язык: Английский

Процитировано

0
Increased Prorenin Expression in the Kidneys May Be Involved in the Abnormal Renal Function Caused by Prolonged Environmental Exposure to Microcystin-LR DOI Creative Commons

Yuuka Hitsuda,

Yoshihito Koto,

Hideaki Kawahara

и другие.

Toxics, Год журнала: 2024, Номер 12(8), С. 547 - 547

Опубликована: Июль 27, 2024

Toxic algae in eutrophic lakes produce cyanotoxic microcystins. Prior research on the effect of microcystin-LR kidney utilized intraperitoneal injections, which did not reflect natural exposure. Oral has focused renal function and histopathology without examining molecular mechanisms. The present study aimed to evaluate mechanism kidneys via oral administration WKAH/HkmSlc rats over 7 weeks, alongside stimulation proximal tubular cells. Although there were no differences concentrations plasma albumin, blood urea nitrogen, creatinine, are parameters function, between control microcystin-LR-administrated rats,

Язык: Английский

Процитировано

3
Co-exposure of microcystin-LR and nitrite induced kidney injury through TLR4/NLRP3/GSDMD-mediated pyroptosis DOI Creative Commons

Yao Guo,

Xingde Du,

Fufang Wang

и другие.

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 281, С. 116629 - 116629

Опубликована: Июнь 24, 2024

The degradation of cyanobacterial blooms releases hazardous contaminants such as microcystin-LR (MC-LR) and nitrite, which may collectively exert toxicity on various bodily systems. To evaluate their individual combined in the kidney, mice were subjected to different concentrations MC-LR and/or nitrite over a 6-month period this study. results revealed that exposure exacerbated renal pathological alterations dysfunction compared either compound alone. Specifically, protein mRNA expression kidney injury biomarkers, molecule 1 (KIM-1) neutrophil gelatinase-associated lipocalin (NGAL), notably increased group. Concurrently, co-exposure remarkedly upregulated levels proinflammatory cytokines TNF-α, IL-6 IL-1β, while decreasing anti-inflammatory cytokine IL-10. Notably, exhibited synergistic effects upregulation IL-1β levels. Moreover, with not only elevated but also pyroptosis biomarkers Gasdermin D (GSDMD), Cleaved-GSDMD. Mechanistic investigations promoted both vivo vitro, possibly through activation TLR4/NLRP3/GSDMD pathway. Pretreatment TLR4 inhibitor NLRP3 effectively suppressed induced by these two toxins HEK293T cells. These findings provide compelling evidence synergistically induces activating Overall, study significantly enhances our comprehension how environmental interact induce harm kidneys, offering promising avenues for identifying therapeutic targets alleviate toxic health.

Язык: Английский

Процитировано

2
MC-LR induced apoptosis in human embryonic kidney (HEK293) cells through activation of TNF-R1/RIPK1 pathway DOI
Yaqi Wang, Yue Yang, Mei Yuan

и другие.

Journal of Toxicology and Environmental Health, Год журнала: 2024, Номер unknown, С. 1 - 10

Опубликована: Дек. 20, 2024

In recent years, the outbreak of cyanobacterial blooms has become increasingly frequent. Microcystin-LR (MC-LR), a metabolite cyanobacteria, poses significant threat to ecosystem and human health. Several studies have demonstrated that MC-LR might induce renal cell apoptosis, as consequence tissue damage. However, molecular mechanisms underlying MC-LR-initiated injury remain be determined. This investigation aimed determine role apoptosis in MC-LR-induced kidney damage its potential using embryonic (HEK293) line. The results TUNEL immunofluorescence assays indicated induced increased HEK293 cells. Compared control, mRNA expression levels RIPK1, caspase-8, TNF-α were elevated following incubation with MC-LR, while level Bcl-2/Bax was decreased. protein TNF-R1, caspase-8 MC-LR-treated Data through activation TNF-R1/RIPK1 pathway, providing new insights into understanding toxic attributed MC-LR.

Язык: Английский

Процитировано

2
Microcystins Exposure and the Risk of Metabolic Syndrome: A Cross-Sectional Study in Central China DOI Creative Commons

Shuidong Feng,

Yalin Zeng,

Fengmei Song

и другие.

Toxins, Год журнала: 2024, Номер 16(12), С. 542 - 542

Опубликована: Дек. 14, 2024

A growing body of evidence indicates that microcystins (MCs) exposure may cause metabolic diseases. However, studies exploring the effects MCs on risk syndrome (MetS) in humans are currently lacking, and underlying mechanisms remain unclear. Here, we conducted a cross-sectional study central China to explore effect serum MetS, assessed mediation inflammation biomarker, white blood cell (WBC) level, this relationship. The relationships among WBC level MetS were using binary logistic linear regression. Mediation analysis was used possible those associations by employing R software (version 4.3.1). Compared lowest quartile MCs, highest had an increased (odds ratio [OR] = 2.10, 95% confidence interval [CI]: 1.19, 3.70), with dose–response relationship (p for trend < 0.05). WBCs mediated 11.14% association between triglyceride (TG) levels, but did not mediate MetS. This firstly reveals is independent factor manner, suggests could partially TG levels.

Язык: Английский

Процитировано

1