The Combined Effect of Two Alternaria Mycotoxins (Alternariol and Alternariol Monomethyl Ether) on Porcine Epithelial Intestinal Cells DOI Creative Commons
Daniela Eliza Marin, Iulian Alexandru Grosu, Gina Cecilia Pistol

и другие.

Agriculture, Год журнала: 2024, Номер 14(9), С. 1478 - 1478

Опубликована: Авг. 30, 2024

Alternariol (AOH) and alternariol monomethyl ether (AME) are secondary metabolites produced by fungi belonging to the genus Alternaria, which generally contaminate fruits but also cereal crops vegetables. The objective of this study was investigate if co-exposure swine epithelial intestinal cell line (IPEC-1) a mixture mycotoxins would cause an increase in toxicity as compared with exposure single toxin. effects individual toxins well those their combination (1:1 ratio), range 1–250 μM, were assessed vitro for viability proliferating IPEC-1 cells then on parameters related oxidative stress. Our results indicate that both AOH AME significantly decreased viability, cytotoxicity induced + higher than toxins. main interaction type ranged from slight synergy AOH-AME affecting 25% (CI = 0.88), evolved into synergistic effect level IL50 0.41) strong at IL90 0.10). In addition, we investigated two low concentrations (2.5 μM 5 μM) administered individually or stress cells. Both can induce reactive oxygen species—ROS (+) cells%—and damage porcine At least additive observed when exposed AOH-AME, consisting percentage ROS oxidation lipids, proteins, DNA toxin effect. A breakdown antioxidant defense after toxins, decrease activity enzymes superoxide dismutase (SOD) catalase (CAT), no synergic resulted conclusion, our data interfere proliferation Moreover, had dose-dependent viability. Also, stronger However, signaling pathways responsible AOH, AME, combinations need further investigations order provide important risk assessments case contamination feed Alternaria

Язык: Английский

Absolute oral bioavailability, quantitative toxicokinetics and metabolite profiling of alternariol and alternariol monomethyl ether in pigs DOI Creative Commons

Danica den Hollander,

Siegrid De Baere,

Celestien Holvoet

и другие.

Archives of Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 26, 2025

Abstract The mycotoxins alternariol (AOH) and monomethyl ether (AME), produced by Alternaria spp . , are common contaminants of food feed a potential threat to animal human health. To date, the most prominent data gaps for their comprehensive risk assessment concern information on in vivo absorption, distribution, metabolism excretion (ADME) toxicokinetic behavior. aim this study was determine absolute oral bioavailability, quantitative characteristics biotransformation AOH AME pigs, using crossover trials with intravenous administration single dose both at 2 mg/kg b.w. Plasma profiles phase I II metabolites were studied vena jugularis UPLC-MS/MS LC-HRMS methods. Furthermore, plasma from portae analyzed presystemic biotransformation. Urine collected urinary metabolite profiles. Results reveal low bioavailability (15%) (9%), caused absorption and/or extensive first-pass liver mainly II, lesser extent metabolites. Quantitative modeling IV showed high total body clearance (12.9 16.8 L/(h*kg b.w.), respectively), volume distribution (4.97 5.15 L/kg b.w., respectively) short elimination half-life 0.16 0.21 h, respectively. These findings may contribute development candidate biomarkers exposure biomonitoring studies since pigs considered suitable model extrapolate humans.

Язык: Английский

Процитировано

0
The Combined Effect of Two Alternaria Mycotoxins (Alternariol and Alternariol Monomethyl Ether) on Porcine Epithelial Intestinal Cells DOI Creative Commons
Daniela Eliza Marin, Iulian Alexandru Grosu, Gina Cecilia Pistol

и другие.

Agriculture, Год журнала: 2024, Номер 14(9), С. 1478 - 1478

Опубликована: Авг. 30, 2024

Alternariol (AOH) and alternariol monomethyl ether (AME) are secondary metabolites produced by fungi belonging to the genus Alternaria, which generally contaminate fruits but also cereal crops vegetables. The objective of this study was investigate if co-exposure swine epithelial intestinal cell line (IPEC-1) a mixture mycotoxins would cause an increase in toxicity as compared with exposure single toxin. effects individual toxins well those their combination (1:1 ratio), range 1–250 μM, were assessed vitro for viability proliferating IPEC-1 cells then on parameters related oxidative stress. Our results indicate that both AOH AME significantly decreased viability, cytotoxicity induced + higher than toxins. main interaction type ranged from slight synergy AOH-AME affecting 25% (CI = 0.88), evolved into synergistic effect level IL50 0.41) strong at IL90 0.10). In addition, we investigated two low concentrations (2.5 μM 5 μM) administered individually or stress cells. Both can induce reactive oxygen species—ROS (+) cells%—and damage porcine At least additive observed when exposed AOH-AME, consisting percentage ROS oxidation lipids, proteins, DNA toxin effect. A breakdown antioxidant defense after toxins, decrease activity enzymes superoxide dismutase (SOD) catalase (CAT), no synergic resulted conclusion, our data interfere proliferation Moreover, had dose-dependent viability. Also, stronger However, signaling pathways responsible AOH, AME, combinations need further investigations order provide important risk assessments case contamination feed Alternaria

Язык: Английский

Процитировано

0